ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas

Detalhes bibliográficos
Autor(a) principal: Freire, Samanta
Data de Publicação: 2023
Outros Autores: Grilo, Teresa, Rodrigues, Bruna, Oliveira, Rui, Esteves, Carla, Marques, António, Poirel, Laurent, Aires-de-Sousa, Marta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158536
Resumo: Funding Information: This work was partially financed by the Portuguese Red Cross, by the University of Fribourg, and by the Swiss National Science Foundation (projects FNS-31003A_163432 and FNS-407240_177381). Publisher Copyright: © 2023 by the authors.
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spelling ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production AreasbivalvescarbapenemasesESBLEscherichia coliKlebsiella pneumoniaePortugalMicrobiologyMicrobiology (medical)VirologySDG 3 - Good Health and Well-beingSDG 14 - Life Below WaterFunding Information: This work was partially financed by the Portuguese Red Cross, by the University of Fribourg, and by the Swiss National Science Foundation (projects FNS-31003A_163432 and FNS-407240_177381). Publisher Copyright: © 2023 by the authors.Bivalves are filter-feeding organisms and biomarkers of bacterial pollution. Our study aimed to analyze the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Escherichia coli among bivalves. A total of 522 bivalve samples were collected along Portuguese shellfish production areas. Homogenized samples were screened for E. coli contamination on corresponding selective plates, allowing for concomitant growth of Klebsiella pneumoniae. E. coli growth was observed in 39% of the samples. Subsequent selective screening identified nine samples (4.4%) contaminated with ESBL producers, corresponding to E. coli (n = 7) and K. pneumoniae (n = 2), while a single carbapenemase-producing K. pneumoniae (0.5%) was identified. ESBLs were all CTX-M-types commonly identified in human isolates, i.e., CTX-M-32 (n = 4), CTX-M-15 (n = 4), and CTX-M-14 (n = 1). The carbapenemase producer harbored the blaGES-5 gene located on a ColE plasmid. Clonality was evaluated by multilocus sequence typing, identifying E. coli backgrounds as ST10, ST23, ST540, ST617, ST746, SLV206, and SLV2325, commonly identified among environmental and human strains. The K. pneumoniae isolates belonged to ST834, ST15, and DLV644. The occurrence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves reveals how the marine environment constitutes a reservoir of critical bacterial pathogens, thus potentially representing a risk to human health.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNFreire, SamantaGrilo, TeresaRodrigues, BrunaOliveira, RuiEsteves, CarlaMarques, AntónioPoirel, LaurentAires-de-Sousa, Marta2023-09-30T22:20:54Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158536eng2076-2607PURE: 72597567https://doi.org/10.3390/microorganisms11020415info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:05Zoai:run.unl.pt:10362/158536Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.862009Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
title ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
spellingShingle ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
Freire, Samanta
bivalves
carbapenemases
ESBL
Escherichia coli
Klebsiella pneumoniae
Portugal
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
title_short ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
title_full ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
title_fullStr ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
title_full_unstemmed ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
title_sort ESBL- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae among Bivalves from Portuguese Shellfish Production Areas
author Freire, Samanta
author_facet Freire, Samanta
Grilo, Teresa
Rodrigues, Bruna
Oliveira, Rui
Esteves, Carla
Marques, António
Poirel, Laurent
Aires-de-Sousa, Marta
author_role author
author2 Grilo, Teresa
Rodrigues, Bruna
Oliveira, Rui
Esteves, Carla
Marques, António
Poirel, Laurent
Aires-de-Sousa, Marta
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Freire, Samanta
Grilo, Teresa
Rodrigues, Bruna
Oliveira, Rui
Esteves, Carla
Marques, António
Poirel, Laurent
Aires-de-Sousa, Marta
dc.subject.por.fl_str_mv bivalves
carbapenemases
ESBL
Escherichia coli
Klebsiella pneumoniae
Portugal
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
topic bivalves
carbapenemases
ESBL
Escherichia coli
Klebsiella pneumoniae
Portugal
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
description Funding Information: This work was partially financed by the Portuguese Red Cross, by the University of Fribourg, and by the Swiss National Science Foundation (projects FNS-31003A_163432 and FNS-407240_177381). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:20:54Z
2023-02
2023-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158536
url http://hdl.handle.net/10362/158536
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 72597567
https://doi.org/10.3390/microorganisms11020415
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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