Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/10341 |
Resumo: | In this study, the crude biosurfactant produced by a Lactobacillus paracasei strain isolated in a Portuguese dairy industry was characterized. The minimum surface tension (41.8mN/m) and the critical micelle concentration (2.5 mg/ml) obtained were found to be similar to the values previously reported for biosurfactants isolated from other lactobacilli. The biosurfactant was found to be stable to pH changes over a range from 6 to 10, being more effective at pH 7, and showed no loss of surface activity after incubation at 60 ◦C for 120 h. Although the biosurfactant chemical composition has not been determined yet, a fraction was isolated through acidic precipitation, which exhibited higher surface activity as compared with the crude biosurfactant. Furthermore, this isolated biosurfactant showed antimicrobial and anti-adhesive activities against several pathogenic microorganisms. In addition, L. paracasei exhibited a strong autoaggregating phenotype, which was maintained after washing and resuspending the cells in PBS, meaning that this attribute must be related to cell surface components and not to excreted factors. The autoaggregation ability exhibited by this strain, together with the antimicrobial and anti-adhesive properties observed for this biosurfactant opens the possibility for its use as an effective probiotic strain. |
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Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracaseiBiosurfactantLactobacillus paracaseiCritical micelle concentrationAntimicrobialAnti-adhesiveAutoaggregationScience & TechnologyIn this study, the crude biosurfactant produced by a Lactobacillus paracasei strain isolated in a Portuguese dairy industry was characterized. The minimum surface tension (41.8mN/m) and the critical micelle concentration (2.5 mg/ml) obtained were found to be similar to the values previously reported for biosurfactants isolated from other lactobacilli. The biosurfactant was found to be stable to pH changes over a range from 6 to 10, being more effective at pH 7, and showed no loss of surface activity after incubation at 60 ◦C for 120 h. Although the biosurfactant chemical composition has not been determined yet, a fraction was isolated through acidic precipitation, which exhibited higher surface activity as compared with the crude biosurfactant. Furthermore, this isolated biosurfactant showed antimicrobial and anti-adhesive activities against several pathogenic microorganisms. In addition, L. paracasei exhibited a strong autoaggregating phenotype, which was maintained after washing and resuspending the cells in PBS, meaning that this attribute must be related to cell surface components and not to excreted factors. The autoaggregation ability exhibited by this strain, together with the antimicrobial and anti-adhesive properties observed for this biosurfactant opens the possibility for its use as an effective probiotic strain.Fundação para a Ciência e a Tecnologia (FCT)under the scope of the Project BIOSURFA: Biosurfactants application for microbial adhesion inhibition in medical devices.ElsevierUniversidade do MinhoGudiña, Eduardo J.Teixeira, J. A.Rodrigues, L. R.2010-032010-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10341eng"Colloids and Surfaces B : biointerfaces". ISSN 0927-7765. 76:1 (Mar. 2010) 298-304.0927-776510.1016/j.colsurfb.2009.11.00820004557info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:50Zoai:repositorium.sdum.uminho.pt:1822/10341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:03.312830Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
title |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
spellingShingle |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei Gudiña, Eduardo J. Biosurfactant Lactobacillus paracasei Critical micelle concentration Antimicrobial Anti-adhesive Autoaggregation Science & Technology |
title_short |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
title_full |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
title_fullStr |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
title_full_unstemmed |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
title_sort |
Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei |
author |
Gudiña, Eduardo J. |
author_facet |
Gudiña, Eduardo J. Teixeira, J. A. Rodrigues, L. R. |
author_role |
author |
author2 |
Teixeira, J. A. Rodrigues, L. R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gudiña, Eduardo J. Teixeira, J. A. Rodrigues, L. R. |
dc.subject.por.fl_str_mv |
Biosurfactant Lactobacillus paracasei Critical micelle concentration Antimicrobial Anti-adhesive Autoaggregation Science & Technology |
topic |
Biosurfactant Lactobacillus paracasei Critical micelle concentration Antimicrobial Anti-adhesive Autoaggregation Science & Technology |
description |
In this study, the crude biosurfactant produced by a Lactobacillus paracasei strain isolated in a Portuguese dairy industry was characterized. The minimum surface tension (41.8mN/m) and the critical micelle concentration (2.5 mg/ml) obtained were found to be similar to the values previously reported for biosurfactants isolated from other lactobacilli. The biosurfactant was found to be stable to pH changes over a range from 6 to 10, being more effective at pH 7, and showed no loss of surface activity after incubation at 60 ◦C for 120 h. Although the biosurfactant chemical composition has not been determined yet, a fraction was isolated through acidic precipitation, which exhibited higher surface activity as compared with the crude biosurfactant. Furthermore, this isolated biosurfactant showed antimicrobial and anti-adhesive activities against several pathogenic microorganisms. In addition, L. paracasei exhibited a strong autoaggregating phenotype, which was maintained after washing and resuspending the cells in PBS, meaning that this attribute must be related to cell surface components and not to excreted factors. The autoaggregation ability exhibited by this strain, together with the antimicrobial and anti-adhesive properties observed for this biosurfactant opens the possibility for its use as an effective probiotic strain. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03 2010-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/10341 |
url |
http://hdl.handle.net/1822/10341 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Colloids and Surfaces B : biointerfaces". ISSN 0927-7765. 76:1 (Mar. 2010) 298-304. 0927-7765 10.1016/j.colsurfb.2009.11.008 20004557 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133111043227648 |