Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450

Detalhes bibliográficos
Autor(a) principal: Gudiña, Eduardo J.
Data de Publicação: 2015
Outros Autores: Fernandes, Elisabete Ramos, Teixeira, J. A., Rodrigues, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/37911
Resumo: In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications.
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spelling Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450BiosurfactantLactic acid bactériaLactobacillus agilisGlycoproteinAntimicrobialAnti-adhesiveScience & TechnologyIn this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications.The authors acknowledge the financial support from the Portuguese Foundation for Science and Technology (FCT) and the European Community fund FEDER, through Program COMPETE, under the scope of the Projects FCOMP-01-0124-FEDER-007025 (PTDC/AMB/68393/2006), PEst-OE/EQB/LA0023/2013, PEST-C/FIS/UI607/2013, RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462), the strategic funding of UID/BIO/04469/2013 unit, and the Projects "BioEnv - Biotechnology and Bioengineering for a sustainable world" and "Matepro - Optimizing Materials and Processes". NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON. 2 - O Novo Norte), QREN, FEDER; and from AdI (Agencia de Inovacao, S.A.), through the project BIOCLEAN - "Development of biosurfactant-based products for surfaces cleaning and disinfection in the food industry" (QREN Ref. 30215).Royal Society of ChemistryUniversidade do MinhoGudiña, Eduardo J.Fernandes, Elisabete RamosTeixeira, J. A.Rodrigues, L. R.2015-10-162015-10-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37911engGudiña, Eduardo J.; Fernandes, E.; Teixeira, J. A.; Rodrigues, L. R., Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. RSC Advances, 5, 90960-90968, 20152046-20692046-206910.1039/c5ra11659ghttp://pubs.rsc.org/en/journals/journalissues/rainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:41Zoai:repositorium.sdum.uminho.pt:1822/37911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:11.830607Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
title Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
spellingShingle Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
Gudiña, Eduardo J.
Biosurfactant
Lactic acid bactéria
Lactobacillus agilis
Glycoprotein
Antimicrobial
Anti-adhesive
Science & Technology
title_short Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
title_full Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
title_fullStr Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
title_full_unstemmed Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
title_sort Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
author Gudiña, Eduardo J.
author_facet Gudiña, Eduardo J.
Fernandes, Elisabete Ramos
Teixeira, J. A.
Rodrigues, L. R.
author_role author
author2 Fernandes, Elisabete Ramos
Teixeira, J. A.
Rodrigues, L. R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gudiña, Eduardo J.
Fernandes, Elisabete Ramos
Teixeira, J. A.
Rodrigues, L. R.
dc.subject.por.fl_str_mv Biosurfactant
Lactic acid bactéria
Lactobacillus agilis
Glycoprotein
Antimicrobial
Anti-adhesive
Science & Technology
topic Biosurfactant
Lactic acid bactéria
Lactobacillus agilis
Glycoprotein
Antimicrobial
Anti-adhesive
Science & Technology
description In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-16
2015-10-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/37911
url http://hdl.handle.net/1822/37911
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gudiña, Eduardo J.; Fernandes, E.; Teixeira, J. A.; Rodrigues, L. R., Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. RSC Advances, 5, 90960-90968, 2015
2046-2069
2046-2069
10.1039/c5ra11659g
http://pubs.rsc.org/en/journals/journalissues/ra
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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