Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/37911 |
Resumo: | In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications. |
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Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450BiosurfactantLactic acid bactériaLactobacillus agilisGlycoproteinAntimicrobialAnti-adhesiveScience & TechnologyIn this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications.The authors acknowledge the financial support from the Portuguese Foundation for Science and Technology (FCT) and the European Community fund FEDER, through Program COMPETE, under the scope of the Projects FCOMP-01-0124-FEDER-007025 (PTDC/AMB/68393/2006), PEst-OE/EQB/LA0023/2013, PEST-C/FIS/UI607/2013, RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462), the strategic funding of UID/BIO/04469/2013 unit, and the Projects "BioEnv - Biotechnology and Bioengineering for a sustainable world" and "Matepro - Optimizing Materials and Processes". NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON. 2 - O Novo Norte), QREN, FEDER; and from AdI (Agencia de Inovacao, S.A.), through the project BIOCLEAN - "Development of biosurfactant-based products for surfaces cleaning and disinfection in the food industry" (QREN Ref. 30215).Royal Society of ChemistryUniversidade do MinhoGudiña, Eduardo J.Fernandes, Elisabete RamosTeixeira, J. A.Rodrigues, L. R.2015-10-162015-10-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37911engGudiña, Eduardo J.; Fernandes, E.; Teixeira, J. A.; Rodrigues, L. R., Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. RSC Advances, 5, 90960-90968, 20152046-20692046-206910.1039/c5ra11659ghttp://pubs.rsc.org/en/journals/journalissues/rainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:41Zoai:repositorium.sdum.uminho.pt:1822/37911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:11.830607Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
title |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
spellingShingle |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 Gudiña, Eduardo J. Biosurfactant Lactic acid bactéria Lactobacillus agilis Glycoprotein Antimicrobial Anti-adhesive Science & Technology |
title_short |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
title_full |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
title_fullStr |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
title_full_unstemmed |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
title_sort |
Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450 |
author |
Gudiña, Eduardo J. |
author_facet |
Gudiña, Eduardo J. Fernandes, Elisabete Ramos Teixeira, J. A. Rodrigues, L. R. |
author_role |
author |
author2 |
Fernandes, Elisabete Ramos Teixeira, J. A. Rodrigues, L. R. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gudiña, Eduardo J. Fernandes, Elisabete Ramos Teixeira, J. A. Rodrigues, L. R. |
dc.subject.por.fl_str_mv |
Biosurfactant Lactic acid bactéria Lactobacillus agilis Glycoprotein Antimicrobial Anti-adhesive Science & Technology |
topic |
Biosurfactant Lactic acid bactéria Lactobacillus agilis Glycoprotein Antimicrobial Anti-adhesive Science & Technology |
description |
In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary chemical characterization by Fourier Transform Infrared Spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg/l. The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa. This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus) in several biomedical applications. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-16 2015-10-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/37911 |
url |
http://hdl.handle.net/1822/37911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gudiña, Eduardo J.; Fernandes, E.; Teixeira, J. A.; Rodrigues, L. R., Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. RSC Advances, 5, 90960-90968, 2015 2046-2069 2046-2069 10.1039/c5ra11659g http://pubs.rsc.org/en/journals/journalissues/ra |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132877022035968 |