On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase

Detalhes bibliográficos
Autor(a) principal: Balcão, Victor M.
Data de Publicação: 1998
Outros Autores: Malcata, F. Xavier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/6653
Resumo: The present communication describes the chemical modification of anhydrous butterfat by interesterification with oleic acid catalyzed by a lipase of Mucor javanicus. Two reactor configurations were tested, a batch-stirred tank reactor containing suspended lipase and a batch-stirred tank reactor in combination with a hollow-fiber membrane module containing adsorbed lipase. The goal of this research was to assess the advantage of using a (hydrophobic) porous support to immobilize the lipase in attempts to engineer butterfat with increased levels of unsaturated fatty acid residues (oleic acid) at the expense of medium-to-long chain saturated fatty acids (myristic and palmitic acids). Reactions were carried out at 40°C in the absence of solvent under controlled water activity, and were monitored by chromatographic assays for free fatty acids. The results obtained indicate that the rate of interesterification using the proposed reactor configuration is enhanced by a factor above 100 relative to that using suspended lipase, for the same protein mass basis. Although hydrolysis of butterfat occurred to some degree, the enzymatic process that uses the hollow-fiber reactor was technically superior to the stirred tank system.
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spelling On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipaseBatch-stirred tank reactorInteresterificationImmobilized enzymeButterfatMembrane bioreactorMucor javanicusThe present communication describes the chemical modification of anhydrous butterfat by interesterification with oleic acid catalyzed by a lipase of Mucor javanicus. Two reactor configurations were tested, a batch-stirred tank reactor containing suspended lipase and a batch-stirred tank reactor in combination with a hollow-fiber membrane module containing adsorbed lipase. The goal of this research was to assess the advantage of using a (hydrophobic) porous support to immobilize the lipase in attempts to engineer butterfat with increased levels of unsaturated fatty acid residues (oleic acid) at the expense of medium-to-long chain saturated fatty acids (myristic and palmitic acids). Reactions were carried out at 40°C in the absence of solvent under controlled water activity, and were monitored by chromatographic assays for free fatty acids. The results obtained indicate that the rate of interesterification using the proposed reactor configuration is enhanced by a factor above 100 relative to that using suspended lipase, for the same protein mass basis. Although hydrolysis of butterfat occurred to some degree, the enzymatic process that uses the hollow-fiber reactor was technically superior to the stirred tank system.John Wiley & Sons, Inc.Veritati - Repositório Institucional da Universidade Católica PortuguesaBalcão, Victor M.Malcata, F. Xavier2011-10-21T12:09:12Z19981998-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6653engBALCÃO, Victor M.; MALCATA, F. Xavier - On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase. Biotechnology and Bioengineering. ISSN 0006-3592. Vol. 60, n.º 1 (1998), p. 114-123info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:54Zoai:repositorio.ucp.pt:10400.14/6653Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:06:03.861769Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
title On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
spellingShingle On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
Balcão, Victor M.
Batch-stirred tank reactor
Interesterification
Immobilized enzyme
Butterfat
Membrane bioreactor
Mucor javanicus
title_short On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
title_full On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
title_fullStr On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
title_full_unstemmed On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
title_sort On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase
author Balcão, Victor M.
author_facet Balcão, Victor M.
Malcata, F. Xavier
author_role author
author2 Malcata, F. Xavier
author2_role author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Balcão, Victor M.
Malcata, F. Xavier
dc.subject.por.fl_str_mv Batch-stirred tank reactor
Interesterification
Immobilized enzyme
Butterfat
Membrane bioreactor
Mucor javanicus
topic Batch-stirred tank reactor
Interesterification
Immobilized enzyme
Butterfat
Membrane bioreactor
Mucor javanicus
description The present communication describes the chemical modification of anhydrous butterfat by interesterification with oleic acid catalyzed by a lipase of Mucor javanicus. Two reactor configurations were tested, a batch-stirred tank reactor containing suspended lipase and a batch-stirred tank reactor in combination with a hollow-fiber membrane module containing adsorbed lipase. The goal of this research was to assess the advantage of using a (hydrophobic) porous support to immobilize the lipase in attempts to engineer butterfat with increased levels of unsaturated fatty acid residues (oleic acid) at the expense of medium-to-long chain saturated fatty acids (myristic and palmitic acids). Reactions were carried out at 40°C in the absence of solvent under controlled water activity, and were monitored by chromatographic assays for free fatty acids. The results obtained indicate that the rate of interesterification using the proposed reactor configuration is enhanced by a factor above 100 relative to that using suspended lipase, for the same protein mass basis. Although hydrolysis of butterfat occurred to some degree, the enzymatic process that uses the hollow-fiber reactor was technically superior to the stirred tank system.
publishDate 1998
dc.date.none.fl_str_mv 1998
1998-01-01T00:00:00Z
2011-10-21T12:09:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/6653
url http://hdl.handle.net/10400.14/6653
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BALCÃO, Victor M.; MALCATA, F. Xavier - On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase. Biotechnology and Bioengineering. ISSN 0006-3592. Vol. 60, n.º 1 (1998), p. 114-123
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons, Inc.
publisher.none.fl_str_mv John Wiley & Sons, Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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