Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/5827 |
Resumo: | INTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases. |
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Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implicationsCross-sectional study of adenoidal biofilms in a paediatric population and its clinical implicationsAdenoidBiofilmsScanning Electron MicroscopyHaemophilusChildrenInfecções RespiratóriasAr e Saúde OcupacionalINTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases.National Institutes of Health [grant numbers 2015DDI1143, 2015Elsevier/ Polish Otorhinlaryngology - Head and Neck Surgery SocietyRepositório Científico do Instituto Nacional de SaúdeSubtil, JoaoBajanca-Lavado, Maria PaulaRodrigues, JoaoDuarte, AidaReis, LuciaNogueira, IsabelJordão, Luisa2019-02-15T14:05:59Z2018-10-012018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/5827engOtolaryngol Pol. 2019; 73(1):22-28. Epub 2018 Oct 01. doi:10.5604/01.3001.0012.52780030-665710.5604/01.3001.0012.5278info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:59Zoai:repositorio.insa.pt:10400.18/5827Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:21.359807Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications Cross-sectional study of adenoidal biofilms in a paediatric population and its clinical implications |
title |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
spellingShingle |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications Subtil, Joao Adenoid Biofilms Scanning Electron Microscopy Haemophilus Children Infecções Respiratórias Ar e Saúde Ocupacional |
title_short |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
title_full |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
title_fullStr |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
title_full_unstemmed |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
title_sort |
Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications |
author |
Subtil, Joao |
author_facet |
Subtil, Joao Bajanca-Lavado, Maria Paula Rodrigues, Joao Duarte, Aida Reis, Lucia Nogueira, Isabel Jordão, Luisa |
author_role |
author |
author2 |
Bajanca-Lavado, Maria Paula Rodrigues, Joao Duarte, Aida Reis, Lucia Nogueira, Isabel Jordão, Luisa |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Subtil, Joao Bajanca-Lavado, Maria Paula Rodrigues, Joao Duarte, Aida Reis, Lucia Nogueira, Isabel Jordão, Luisa |
dc.subject.por.fl_str_mv |
Adenoid Biofilms Scanning Electron Microscopy Haemophilus Children Infecções Respiratórias Ar e Saúde Ocupacional |
topic |
Adenoid Biofilms Scanning Electron Microscopy Haemophilus Children Infecções Respiratórias Ar e Saúde Ocupacional |
description |
INTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-01 2018-10-01T00:00:00Z 2019-02-15T14:05:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/5827 |
url |
http://hdl.handle.net/10400.18/5827 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Otolaryngol Pol. 2019; 73(1):22-28. Epub 2018 Oct 01. doi:10.5604/01.3001.0012.5278 0030-6657 10.5604/01.3001.0012.5278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/ Polish Otorhinlaryngology - Head and Neck Surgery Society |
publisher.none.fl_str_mv |
Elsevier/ Polish Otorhinlaryngology - Head and Neck Surgery Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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