Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices

Detalhes bibliográficos
Autor(a) principal: Fernandes, Ellen Eduarda [UNESP]
Data de Publicação: 2023
Outros Autores: Oliveira, Daniela Fernandes Lobo Molica [UNESP], Jóias, Renata Pilli [UNESP], Diniz, Pamela Aparecida [UNESP], De Barros, Patrícia Pimentel, Jorge, Antônio Olavo Cardoso [UNESP], DE Oliveira, Wagner [UNESP], Rode, Sigmar De Mello [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4322/bds.2023.e3440
http://hdl.handle.net/11449/248810
Resumo: Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms.
id UNSP_538865352619b00017db3b23c2051259
oai_identifier_str oai:repositorio.unesp.br:11449/248810
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devicesAnálise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixosBiofilmsCandida albicansOrthodontic appliancesOrthodontic bracketsScanning electron microscopyObjective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms.Universidade Estadual PaulistaUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SPEscola Multicampi de Ciências Médicas Universidade Federal do Rio Grande do Norte (UFRN), RNUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SPUniversidade Estadual Paulista (UNESP)Universidade Federal do Rio Grande do Norte (UFRN)Fernandes, Ellen Eduarda [UNESP]Oliveira, Daniela Fernandes Lobo Molica [UNESP]Jóias, Renata Pilli [UNESP]Diniz, Pamela Aparecida [UNESP]De Barros, Patrícia PimentelJorge, Antônio Olavo Cardoso [UNESP]DE Oliveira, Wagner [UNESP]Rode, Sigmar De Mello [UNESP]2023-07-29T13:54:22Z2023-07-29T13:54:22Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4322/bds.2023.e3440Brazilian Dental Science, v. 26, n. 2, 2023.2178-6011http://hdl.handle.net/11449/24881010.4322/bds.2023.e34402-s2.0-85159065638Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Scienceinfo:eu-repo/semantics/openAccess2023-07-29T13:54:22Zoai:repositorio.unesp.br:11449/248810Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:22:54.617730Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
Análise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixos
title Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
spellingShingle Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
Fernandes, Ellen Eduarda [UNESP]
Biofilms
Candida albicans
Orthodontic appliances
Orthodontic brackets
Scanning electron microscopy
title_short Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_full Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_fullStr Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_full_unstemmed Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_sort Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
author Fernandes, Ellen Eduarda [UNESP]
author_facet Fernandes, Ellen Eduarda [UNESP]
Oliveira, Daniela Fernandes Lobo Molica [UNESP]
Jóias, Renata Pilli [UNESP]
Diniz, Pamela Aparecida [UNESP]
De Barros, Patrícia Pimentel
Jorge, Antônio Olavo Cardoso [UNESP]
DE Oliveira, Wagner [UNESP]
Rode, Sigmar De Mello [UNESP]
author_role author
author2 Oliveira, Daniela Fernandes Lobo Molica [UNESP]
Jóias, Renata Pilli [UNESP]
Diniz, Pamela Aparecida [UNESP]
De Barros, Patrícia Pimentel
Jorge, Antônio Olavo Cardoso [UNESP]
DE Oliveira, Wagner [UNESP]
Rode, Sigmar De Mello [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal do Rio Grande do Norte (UFRN)
dc.contributor.author.fl_str_mv Fernandes, Ellen Eduarda [UNESP]
Oliveira, Daniela Fernandes Lobo Molica [UNESP]
Jóias, Renata Pilli [UNESP]
Diniz, Pamela Aparecida [UNESP]
De Barros, Patrícia Pimentel
Jorge, Antônio Olavo Cardoso [UNESP]
DE Oliveira, Wagner [UNESP]
Rode, Sigmar De Mello [UNESP]
dc.subject.por.fl_str_mv Biofilms
Candida albicans
Orthodontic appliances
Orthodontic brackets
Scanning electron microscopy
topic Biofilms
Candida albicans
Orthodontic appliances
Orthodontic brackets
Scanning electron microscopy
description Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:54:22Z
2023-07-29T13:54:22Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4322/bds.2023.e3440
Brazilian Dental Science, v. 26, n. 2, 2023.
2178-6011
http://hdl.handle.net/11449/248810
10.4322/bds.2023.e3440
2-s2.0-85159065638
url http://dx.doi.org/10.4322/bds.2023.e3440
http://hdl.handle.net/11449/248810
identifier_str_mv Brazilian Dental Science, v. 26, n. 2, 2023.
2178-6011
10.4322/bds.2023.e3440
2-s2.0-85159065638
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Dental Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129421787267072