Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4322/bds.2023.e3440 http://hdl.handle.net/11449/248810 |
Resumo: | Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. |
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Repositório Institucional da UNESP |
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Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devicesAnálise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixosBiofilmsCandida albicansOrthodontic appliancesOrthodontic bracketsScanning electron microscopyObjective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms.Universidade Estadual PaulistaUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SPEscola Multicampi de Ciências Médicas Universidade Federal do Rio Grande do Norte (UFRN), RNUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SPUniversidade Estadual Paulista (UNESP)Universidade Federal do Rio Grande do Norte (UFRN)Fernandes, Ellen Eduarda [UNESP]Oliveira, Daniela Fernandes Lobo Molica [UNESP]Jóias, Renata Pilli [UNESP]Diniz, Pamela Aparecida [UNESP]De Barros, Patrícia PimentelJorge, Antônio Olavo Cardoso [UNESP]DE Oliveira, Wagner [UNESP]Rode, Sigmar De Mello [UNESP]2023-07-29T13:54:22Z2023-07-29T13:54:22Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4322/bds.2023.e3440Brazilian Dental Science, v. 26, n. 2, 2023.2178-6011http://hdl.handle.net/11449/24881010.4322/bds.2023.e34402-s2.0-85159065638Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Scienceinfo:eu-repo/semantics/openAccess2023-07-29T13:54:22Zoai:repositorio.unesp.br:11449/248810Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:22:54.617730Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices Análise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixos |
title |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
spellingShingle |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices Fernandes, Ellen Eduarda [UNESP] Biofilms Candida albicans Orthodontic appliances Orthodontic brackets Scanning electron microscopy |
title_short |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_full |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_fullStr |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_full_unstemmed |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_sort |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
author |
Fernandes, Ellen Eduarda [UNESP] |
author_facet |
Fernandes, Ellen Eduarda [UNESP] Oliveira, Daniela Fernandes Lobo Molica [UNESP] Jóias, Renata Pilli [UNESP] Diniz, Pamela Aparecida [UNESP] De Barros, Patrícia Pimentel Jorge, Antônio Olavo Cardoso [UNESP] DE Oliveira, Wagner [UNESP] Rode, Sigmar De Mello [UNESP] |
author_role |
author |
author2 |
Oliveira, Daniela Fernandes Lobo Molica [UNESP] Jóias, Renata Pilli [UNESP] Diniz, Pamela Aparecida [UNESP] De Barros, Patrícia Pimentel Jorge, Antônio Olavo Cardoso [UNESP] DE Oliveira, Wagner [UNESP] Rode, Sigmar De Mello [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal do Rio Grande do Norte (UFRN) |
dc.contributor.author.fl_str_mv |
Fernandes, Ellen Eduarda [UNESP] Oliveira, Daniela Fernandes Lobo Molica [UNESP] Jóias, Renata Pilli [UNESP] Diniz, Pamela Aparecida [UNESP] De Barros, Patrícia Pimentel Jorge, Antônio Olavo Cardoso [UNESP] DE Oliveira, Wagner [UNESP] Rode, Sigmar De Mello [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida albicans Orthodontic appliances Orthodontic brackets Scanning electron microscopy |
topic |
Biofilms Candida albicans Orthodontic appliances Orthodontic brackets Scanning electron microscopy |
description |
Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:54:22Z 2023-07-29T13:54:22Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4322/bds.2023.e3440 Brazilian Dental Science, v. 26, n. 2, 2023. 2178-6011 http://hdl.handle.net/11449/248810 10.4322/bds.2023.e3440 2-s2.0-85159065638 |
url |
http://dx.doi.org/10.4322/bds.2023.e3440 http://hdl.handle.net/11449/248810 |
identifier_str_mv |
Brazilian Dental Science, v. 26, n. 2, 2023. 2178-6011 10.4322/bds.2023.e3440 2-s2.0-85159065638 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129421787267072 |