Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/15792 https://doi.org/10.1016/j.jep.2008.06.012 |
Resumo: | Ethnopharmacological relevance: Essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. Daucus carota L. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils. Aim of the study: In the present study the composition and the antifungal activity of the oils of Daucus carota L. subsp. halophilus (Brot.) A. Pujadas (Apiaceae), an endemic plant from Portugal, were evaluated. Moreover, their cytotoxicity in mouse skin dendritic cells at concentration showing significant antifungal activity was also evaluated. Material and methods: The oilswere investigated by GC and GC-MS and the antifungal activity (MIC and MLC) were evaluated against yeasts, dermatophyte and Aspergillus strains. Assessment of cell viability was made by the MTT assay. Results: The results showed large variations in the compositions during ontogenesis, particularly in the amounts of elemicin that increased significantly in the ripe umbels (5.9% vs. 31.0%). The results also demonstrated that the oil with high amounts of elemicin, which have stronger antifungal activity, showed no cytotoxic effect, at concentrations ranging from 0.16 to 0.64 l/ml, for as long as 24h. Conclusion: It is possible to find appropriate doses of Daucus carota oil showing both antifungal activity and very low detrimental effect on mammalian cells. |
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Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicityOleos EssenciaisActividade antifungicaCitotoxicidade in vitroEthnopharmacological relevance: Essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. Daucus carota L. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils. Aim of the study: In the present study the composition and the antifungal activity of the oils of Daucus carota L. subsp. halophilus (Brot.) A. Pujadas (Apiaceae), an endemic plant from Portugal, were evaluated. Moreover, their cytotoxicity in mouse skin dendritic cells at concentration showing significant antifungal activity was also evaluated. Material and methods: The oilswere investigated by GC and GC-MS and the antifungal activity (MIC and MLC) were evaluated against yeasts, dermatophyte and Aspergillus strains. Assessment of cell viability was made by the MTT assay. Results: The results showed large variations in the compositions during ontogenesis, particularly in the amounts of elemicin that increased significantly in the ripe umbels (5.9% vs. 31.0%). The results also demonstrated that the oil with high amounts of elemicin, which have stronger antifungal activity, showed no cytotoxic effect, at concentrations ranging from 0.16 to 0.64 l/ml, for as long as 24h. Conclusion: It is possible to find appropriate doses of Daucus carota oil showing both antifungal activity and very low detrimental effect on mammalian cells.Elsevier2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/15792http://hdl.handle.net/10316/15792https://doi.org/10.1016/j.jep.2008.06.012engTavares, Ana CristinaGonçalves, Maria JoséCavaleiro, CarlosCruz, Maria TeresaLopes, Maria CelesteCanhoto, JorgeRibeiro Salgueiro, Lígiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-21T08:08:53Zoai:estudogeral.uc.pt:10316/15792Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:58.058531Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
title |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
spellingShingle |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity Tavares, Ana Cristina Oleos Essenciais Actividade antifungica Citotoxicidade in vitro |
title_short |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
title_full |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
title_fullStr |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
title_full_unstemmed |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
title_sort |
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity |
author |
Tavares, Ana Cristina |
author_facet |
Tavares, Ana Cristina Gonçalves, Maria José Cavaleiro, Carlos Cruz, Maria Teresa Lopes, Maria Celeste Canhoto, Jorge Ribeiro Salgueiro, Lígia |
author_role |
author |
author2 |
Gonçalves, Maria José Cavaleiro, Carlos Cruz, Maria Teresa Lopes, Maria Celeste Canhoto, Jorge Ribeiro Salgueiro, Lígia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Tavares, Ana Cristina Gonçalves, Maria José Cavaleiro, Carlos Cruz, Maria Teresa Lopes, Maria Celeste Canhoto, Jorge Ribeiro Salgueiro, Lígia |
dc.subject.por.fl_str_mv |
Oleos Essenciais Actividade antifungica Citotoxicidade in vitro |
topic |
Oleos Essenciais Actividade antifungica Citotoxicidade in vitro |
description |
Ethnopharmacological relevance: Essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. Daucus carota L. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils. Aim of the study: In the present study the composition and the antifungal activity of the oils of Daucus carota L. subsp. halophilus (Brot.) A. Pujadas (Apiaceae), an endemic plant from Portugal, were evaluated. Moreover, their cytotoxicity in mouse skin dendritic cells at concentration showing significant antifungal activity was also evaluated. Material and methods: The oilswere investigated by GC and GC-MS and the antifungal activity (MIC and MLC) were evaluated against yeasts, dermatophyte and Aspergillus strains. Assessment of cell viability was made by the MTT assay. Results: The results showed large variations in the compositions during ontogenesis, particularly in the amounts of elemicin that increased significantly in the ripe umbels (5.9% vs. 31.0%). The results also demonstrated that the oil with high amounts of elemicin, which have stronger antifungal activity, showed no cytotoxic effect, at concentrations ranging from 0.16 to 0.64 l/ml, for as long as 24h. Conclusion: It is possible to find appropriate doses of Daucus carota oil showing both antifungal activity and very low detrimental effect on mammalian cells. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/15792 http://hdl.handle.net/10316/15792 https://doi.org/10.1016/j.jep.2008.06.012 |
url |
http://hdl.handle.net/10316/15792 https://doi.org/10.1016/j.jep.2008.06.012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133845418672128 |