Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4650 |
Resumo: | Three‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays. |
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Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures modelsCancerIn vitro modelsLOT3D MCTSTumor microenvironmentThree‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays.WileyuBibliorumCosta, Elisabete C.Gaspar, Vítor Manuel AbreuCoutinho, PaulaCorreia, Ilídio Joaquim Sobreira2018-03-20T10:38:37Z2014-02-252014-02-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4650engCosta, E.C., Gaspar, V.M., Coutinho, P. e Correia, I.J. (2014) “Optimization of Liquid Overlay Technique to Formulate Heterogenic 3D Co-Cultures models”, Biotechnology and Bioengineering, Vol. 111 (8), pp. 1672-168510.1002/bit.25210metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:50Zoai:ubibliorum.ubi.pt:10400.6/4650Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:42.618644Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
title |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
spellingShingle |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models Costa, Elisabete C. Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
title_short |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
title_full |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
title_fullStr |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
title_full_unstemmed |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
title_sort |
Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models |
author |
Costa, Elisabete C. |
author_facet |
Costa, Elisabete C. Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Costa, Elisabete C. Gaspar, Vítor Manuel Abreu Coutinho, Paula Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
topic |
Cancer In vitro models LOT 3D MCTS Tumor microenvironment |
description |
Three‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-25 2014-02-25T00:00:00Z 2018-03-20T10:38:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4650 |
url |
http://hdl.handle.net/10400.6/4650 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa, E.C., Gaspar, V.M., Coutinho, P. e Correia, I.J. (2014) “Optimization of Liquid Overlay Technique to Formulate Heterogenic 3D Co-Cultures models”, Biotechnology and Bioengineering, Vol. 111 (8), pp. 1672-1685 10.1002/bit.25210 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136354451324928 |