Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Bruna
Data de Publicação: 2020
Outros Autores: Bernardo, Ruben, Wang, Can, Schroeder, Markus, Pedro, Nuno A., Butler, Geraldine, Azeredo, Joana, Henriques, Mariana, Mira, Nuno Pereira, Silva, Sónia Carina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64979
Resumo: Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.ijmm.2020.151414.
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spelling Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysisVulvovaginal candidiasisvaginal environmentbiofilm regulatorsexpression profileScience & TechnologySupplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.ijmm.2020.151414.Vulvovaginal candidiasis (VVC) caused by Candida albicans is a common disease worldwide. A very important C. albicans virulence factor is its ability to form biofilms on epithelium and/or on intrauterine devices promoting VVC. It has been shown that VVC has a hormonal dependency and that progesterone affects virulence traits of C. albicans cells. To understand how the acidic environment (pH 4) and progesterone (either alone and in combination) modulate C. albicans response during formation of biofilm, a transcriptomic analysis was performed together with characterization of the biofilm properties. Compared to planktonic cells, acidic biofilm-cells exhibited major changes in their transcriptome, including modifications in the expression of 286 genes that were not previously associated with biofilm formation in C. albicans. The vast majority of the genes up-regulated in the acidic biofilm cells (including those uniquely identified in our study) are known targets of Sfl1, and consistently, Sfl1 deletion is herein shown to impair the formation of acidic biofilms (pH4). Under the acidic conditions used, the presence of progesterone reduced C. albicans biofilm biomass and structural cohesion. Transcriptomic analysis of biofilms developed in the presence of progesterone led to the identification of 65 down-regulated genes including, among others, the regulator Tec1 and several of its target genes, suggesting that the function of this transcription factor is inhibited by the presence of the hormone. Additionally, progesterone reduced the susceptibility of biofilm cells to fluconazole, consistent with an up-regulation of efflux pumps. Overall, the results of this study show that progesterone modulates C. albicans biofilm formation and genomic expression under acidic conditions, which may have implications for C. albicans pathogenicity in the vaginal environment.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Funding received by iBB–Institute for Bioengineering and Biosciences from FCT (UID/BIO/04565/2013), from Programa Operacional Regional de Lisboa 2020 Project No. 007317 is also acknowledged. Support from FCT to NAP through the doctoral program Applied and Environmental Microbiology (PD/BD/143026/2018) is also acknowledged. B. Gonçalves is recipient of a PhD grant supported by FCT (SFRH/BD/111645/2015). G. Butler was supported by Science Foundation Ireland (12/IA/1343).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoGonçalves, BrunaBernardo, RubenWang, CanSchroeder, MarkusPedro, Nuno A.Butler, GeraldineAzeredo, JoanaHenriques, MarianaMira, Nuno PereiraSilva, Sónia Carina2020-042020-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64979engGonçalves, Bruna; Bernardo, Ruben; Wang, Can; Schroeder, Markus; Pedro, Nuno A.; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno Pereira; Silva, Sónia Carina, Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis. International Journal of Medical Microbiology, 310(3), 151414, 20201438-42211618-060710.1016/j.ijmm.2020.15141432173268http://www.journals.elsevier.com/international-journal-of-medical-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:10Zoai:repositorium.sdum.uminho.pt:1822/64979Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:10.928409Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
title Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
spellingShingle Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
Gonçalves, Bruna
Vulvovaginal candidiasis
vaginal environment
biofilm regulators
expression profile
Science & Technology
title_short Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
title_full Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
title_fullStr Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
title_full_unstemmed Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
title_sort Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis
author Gonçalves, Bruna
author_facet Gonçalves, Bruna
Bernardo, Ruben
Wang, Can
Schroeder, Markus
Pedro, Nuno A.
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno Pereira
Silva, Sónia Carina
author_role author
author2 Bernardo, Ruben
Wang, Can
Schroeder, Markus
Pedro, Nuno A.
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno Pereira
Silva, Sónia Carina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, Bruna
Bernardo, Ruben
Wang, Can
Schroeder, Markus
Pedro, Nuno A.
Butler, Geraldine
Azeredo, Joana
Henriques, Mariana
Mira, Nuno Pereira
Silva, Sónia Carina
dc.subject.por.fl_str_mv Vulvovaginal candidiasis
vaginal environment
biofilm regulators
expression profile
Science & Technology
topic Vulvovaginal candidiasis
vaginal environment
biofilm regulators
expression profile
Science & Technology
description Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.ijmm.2020.151414.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
2020-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64979
url http://hdl.handle.net/1822/64979
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gonçalves, Bruna; Bernardo, Ruben; Wang, Can; Schroeder, Markus; Pedro, Nuno A.; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno Pereira; Silva, Sónia Carina, Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis. International Journal of Medical Microbiology, 310(3), 151414, 2020
1438-4221
1618-0607
10.1016/j.ijmm.2020.151414
32173268
http://www.journals.elsevier.com/international-journal-of-medical-microbiology/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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