Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata

Detalhes bibliográficos
Autor(a) principal: Bonifacio, Bruna Vidal [UNESP]
Data de Publicação: 2019
Outros Autores: Machado Vila, Taissa Vieira, Masiero, Isadora Fantacini [UNESP], Silva, Patricia Bento da [UNESP], Silva, Isabel Cristiane da [UNESP], Lopes, Erica de Oliveira [UNESP], Santos Ramos, Matheus Aparecido dos [UNESP], Souza, Leonardo Perez de [UNESP], Vilegas, Wagner [UNESP], Pavan, Fernando Rogerio [UNESP], Chorilli, Marlus [UNESP], Lopez-Ribot, Jose Luis, Bauab, Tais Maria [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2019.02642
http://hdl.handle.net/11449/196367
Resumo: We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal.
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spelling Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrataAstronium urundeuvaCandida spbiofilmcheckerboardresistancevulvovaginal candidiasisWe have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Institute of Dental and Craniofacial ResearchNational Institute of Allergy and Infectious DiseasesMargaret Batts Tobin Foundation, San Antonio, TX, United StatesSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilUniv Texas San Antonio, Dept Biol, San Antonio, TX 78249 USAUniv Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX 78249 USASao Paulo State Univ UNESP, Inst Chem, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, Sao Vicente, BrazilSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Chem, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, Sao Vicente, BrazilFAPESP: 2009/52237-9FAPESP: 2013/25432-0FAPESP: 2013/25121-5FAPESP: 2015/19011-8CAPES: 001National Institute of Dental and Craniofacial Research: R01DE023510National Institute of Allergy and Infectious Diseases: R01AI119554Frontiers Media SaUniversidade Estadual Paulista (Unesp)Univ Texas San AntonioBonifacio, Bruna Vidal [UNESP]Machado Vila, Taissa VieiraMasiero, Isadora Fantacini [UNESP]Silva, Patricia Bento da [UNESP]Silva, Isabel Cristiane da [UNESP]Lopes, Erica de Oliveira [UNESP]Santos Ramos, Matheus Aparecido dos [UNESP]Souza, Leonardo Perez de [UNESP]Vilegas, Wagner [UNESP]Pavan, Fernando Rogerio [UNESP]Chorilli, Marlus [UNESP]Lopez-Ribot, Jose LuisBauab, Tais Maria [UNESP]2020-12-10T19:42:22Z2020-12-10T19:42:22Z2019-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.3389/fmicb.2019.02642Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019.1664-302Xhttp://hdl.handle.net/11449/19636710.3389/fmicb.2019.02642WOS:000499895000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T07:27:32Zoai:repositorio.unesp.br:11449/196367Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:58:49.326690Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
title Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
spellingShingle Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
Bonifacio, Bruna Vidal [UNESP]
Astronium urundeuva
Candida sp
biofilm
checkerboard
resistance
vulvovaginal candidiasis
title_short Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
title_full Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
title_fullStr Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
title_full_unstemmed Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
title_sort Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
author Bonifacio, Bruna Vidal [UNESP]
author_facet Bonifacio, Bruna Vidal [UNESP]
Machado Vila, Taissa Vieira
Masiero, Isadora Fantacini [UNESP]
Silva, Patricia Bento da [UNESP]
Silva, Isabel Cristiane da [UNESP]
Lopes, Erica de Oliveira [UNESP]
Santos Ramos, Matheus Aparecido dos [UNESP]
Souza, Leonardo Perez de [UNESP]
Vilegas, Wagner [UNESP]
Pavan, Fernando Rogerio [UNESP]
Chorilli, Marlus [UNESP]
Lopez-Ribot, Jose Luis
Bauab, Tais Maria [UNESP]
author_role author
author2 Machado Vila, Taissa Vieira
Masiero, Isadora Fantacini [UNESP]
Silva, Patricia Bento da [UNESP]
Silva, Isabel Cristiane da [UNESP]
Lopes, Erica de Oliveira [UNESP]
Santos Ramos, Matheus Aparecido dos [UNESP]
Souza, Leonardo Perez de [UNESP]
Vilegas, Wagner [UNESP]
Pavan, Fernando Rogerio [UNESP]
Chorilli, Marlus [UNESP]
Lopez-Ribot, Jose Luis
Bauab, Tais Maria [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Texas San Antonio
dc.contributor.author.fl_str_mv Bonifacio, Bruna Vidal [UNESP]
Machado Vila, Taissa Vieira
Masiero, Isadora Fantacini [UNESP]
Silva, Patricia Bento da [UNESP]
Silva, Isabel Cristiane da [UNESP]
Lopes, Erica de Oliveira [UNESP]
Santos Ramos, Matheus Aparecido dos [UNESP]
Souza, Leonardo Perez de [UNESP]
Vilegas, Wagner [UNESP]
Pavan, Fernando Rogerio [UNESP]
Chorilli, Marlus [UNESP]
Lopez-Ribot, Jose Luis
Bauab, Tais Maria [UNESP]
dc.subject.por.fl_str_mv Astronium urundeuva
Candida sp
biofilm
checkerboard
resistance
vulvovaginal candidiasis
topic Astronium urundeuva
Candida sp
biofilm
checkerboard
resistance
vulvovaginal candidiasis
description We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-15
2020-12-10T19:42:22Z
2020-12-10T19:42:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2019.02642
Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019.
1664-302X
http://hdl.handle.net/11449/196367
10.3389/fmicb.2019.02642
WOS:000499895000001
url http://dx.doi.org/10.3389/fmicb.2019.02642
http://hdl.handle.net/11449/196367
identifier_str_mv Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019.
1664-302X
10.3389/fmicb.2019.02642
WOS:000499895000001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers In Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Frontiers Media Sa
publisher.none.fl_str_mv Frontiers Media Sa
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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