Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2019.02642 http://hdl.handle.net/11449/196367 |
Resumo: | We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal. |
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Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrataAstronium urundeuvaCandida spbiofilmcheckerboardresistancevulvovaginal candidiasisWe have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Institute of Dental and Craniofacial ResearchNational Institute of Allergy and Infectious DiseasesMargaret Batts Tobin Foundation, San Antonio, TX, United StatesSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilUniv Texas San Antonio, Dept Biol, San Antonio, TX 78249 USAUniv Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX 78249 USASao Paulo State Univ UNESP, Inst Chem, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, Sao Vicente, BrazilSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Chem, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, Sao Vicente, BrazilFAPESP: 2009/52237-9FAPESP: 2013/25432-0FAPESP: 2013/25121-5FAPESP: 2015/19011-8CAPES: 001National Institute of Dental and Craniofacial Research: R01DE023510National Institute of Allergy and Infectious Diseases: R01AI119554Frontiers Media SaUniversidade Estadual Paulista (Unesp)Univ Texas San AntonioBonifacio, Bruna Vidal [UNESP]Machado Vila, Taissa VieiraMasiero, Isadora Fantacini [UNESP]Silva, Patricia Bento da [UNESP]Silva, Isabel Cristiane da [UNESP]Lopes, Erica de Oliveira [UNESP]Santos Ramos, Matheus Aparecido dos [UNESP]Souza, Leonardo Perez de [UNESP]Vilegas, Wagner [UNESP]Pavan, Fernando Rogerio [UNESP]Chorilli, Marlus [UNESP]Lopez-Ribot, Jose LuisBauab, Tais Maria [UNESP]2020-12-10T19:42:22Z2020-12-10T19:42:22Z2019-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.3389/fmicb.2019.02642Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019.1664-302Xhttp://hdl.handle.net/11449/19636710.3389/fmicb.2019.02642WOS:000499895000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T07:27:32Zoai:repositorio.unesp.br:11449/196367Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:58:49.326690Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
title |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
spellingShingle |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata Bonifacio, Bruna Vidal [UNESP] Astronium urundeuva Candida sp biofilm checkerboard resistance vulvovaginal candidiasis |
title_short |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
title_full |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
title_fullStr |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
title_full_unstemmed |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
title_sort |
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata |
author |
Bonifacio, Bruna Vidal [UNESP] |
author_facet |
Bonifacio, Bruna Vidal [UNESP] Machado Vila, Taissa Vieira Masiero, Isadora Fantacini [UNESP] Silva, Patricia Bento da [UNESP] Silva, Isabel Cristiane da [UNESP] Lopes, Erica de Oliveira [UNESP] Santos Ramos, Matheus Aparecido dos [UNESP] Souza, Leonardo Perez de [UNESP] Vilegas, Wagner [UNESP] Pavan, Fernando Rogerio [UNESP] Chorilli, Marlus [UNESP] Lopez-Ribot, Jose Luis Bauab, Tais Maria [UNESP] |
author_role |
author |
author2 |
Machado Vila, Taissa Vieira Masiero, Isadora Fantacini [UNESP] Silva, Patricia Bento da [UNESP] Silva, Isabel Cristiane da [UNESP] Lopes, Erica de Oliveira [UNESP] Santos Ramos, Matheus Aparecido dos [UNESP] Souza, Leonardo Perez de [UNESP] Vilegas, Wagner [UNESP] Pavan, Fernando Rogerio [UNESP] Chorilli, Marlus [UNESP] Lopez-Ribot, Jose Luis Bauab, Tais Maria [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Texas San Antonio |
dc.contributor.author.fl_str_mv |
Bonifacio, Bruna Vidal [UNESP] Machado Vila, Taissa Vieira Masiero, Isadora Fantacini [UNESP] Silva, Patricia Bento da [UNESP] Silva, Isabel Cristiane da [UNESP] Lopes, Erica de Oliveira [UNESP] Santos Ramos, Matheus Aparecido dos [UNESP] Souza, Leonardo Perez de [UNESP] Vilegas, Wagner [UNESP] Pavan, Fernando Rogerio [UNESP] Chorilli, Marlus [UNESP] Lopez-Ribot, Jose Luis Bauab, Tais Maria [UNESP] |
dc.subject.por.fl_str_mv |
Astronium urundeuva Candida sp biofilm checkerboard resistance vulvovaginal candidiasis |
topic |
Astronium urundeuva Candida sp biofilm checkerboard resistance vulvovaginal candidiasis |
description |
We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-15 2020-12-10T19:42:22Z 2020-12-10T19:42:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2019.02642 Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019. 1664-302X http://hdl.handle.net/11449/196367 10.3389/fmicb.2019.02642 WOS:000499895000001 |
url |
http://dx.doi.org/10.3389/fmicb.2019.02642 http://hdl.handle.net/11449/196367 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 12 p., 2019. 1664-302X 10.3389/fmicb.2019.02642 WOS:000499895000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129145635340288 |