What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/18135 |
Resumo: | Macroalgal surface constitutes a peculiar ecological niche and an advantageous substratum for microorganisms able to degrade the wide diversity of algal glycans. The degrading enzymatic activities of macroalgal epiphytes are of paramount interest for the industrial by-product sector and biomass resource applications. We characterized the polysaccharide hydrolytic profle of bacterial isolates obtained from three macroalgal species: the red macroalgae Asparagopsis taxiformis and Sphaerococcus coronopifolius (Rhodophyceae) and the brown Halopteris scoparia (Phaeophyceae), sampled in South Portugal. Bacterial enrichment cultures supplemented with chlorinated aliphatic compounds, typically released by marine algae, were established using as inoculum the decaying biomass of the three macroalgae, obtaining a collection of 634 bacterial strains. Although collected from the same site and exposed to the same seawater seeding microbiota, macroalgal cultivable bacterial communities in terms of functional and phylogenetic diversity showed host specifcity. Isolates were tested for the hydrolysis of starch, pectin, alginate and agar, exhibiting a diferent hydrolytic potential according to their host: A. taxiformis showed the highest percentage of active isolates (91%), followed by S. coronopifolius (54%) and H. scoparia (46%). Only 30% of the isolates were able to degrade starch, while the other polymers were degraded by 55–58% of the isolates. Interestingly, several isolates showed promiscuous capacities to hydrolyze more than one polysaccharide. The isolate functional fngerprint was statistically correlated to bacterial phylogeny, host species and enrichment medium. In conclusion, this work depicts macroalgae as holobionts with an associated microbiota of interest for blue biotechnologies, suggesting isolation strategies and bacterial targets for polysaccharidases’ discovery |
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What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteriaBlue biotechnologyEpibiotaSeaweedsBioprospectingCultivable microbiotaPolysaccharidasesMacroalgal surface constitutes a peculiar ecological niche and an advantageous substratum for microorganisms able to degrade the wide diversity of algal glycans. The degrading enzymatic activities of macroalgal epiphytes are of paramount interest for the industrial by-product sector and biomass resource applications. We characterized the polysaccharide hydrolytic profle of bacterial isolates obtained from three macroalgal species: the red macroalgae Asparagopsis taxiformis and Sphaerococcus coronopifolius (Rhodophyceae) and the brown Halopteris scoparia (Phaeophyceae), sampled in South Portugal. Bacterial enrichment cultures supplemented with chlorinated aliphatic compounds, typically released by marine algae, were established using as inoculum the decaying biomass of the three macroalgae, obtaining a collection of 634 bacterial strains. Although collected from the same site and exposed to the same seawater seeding microbiota, macroalgal cultivable bacterial communities in terms of functional and phylogenetic diversity showed host specifcity. Isolates were tested for the hydrolysis of starch, pectin, alginate and agar, exhibiting a diferent hydrolytic potential according to their host: A. taxiformis showed the highest percentage of active isolates (91%), followed by S. coronopifolius (54%) and H. scoparia (46%). Only 30% of the isolates were able to degrade starch, while the other polymers were degraded by 55–58% of the isolates. Interestingly, several isolates showed promiscuous capacities to hydrolyze more than one polysaccharide. The isolate functional fngerprint was statistically correlated to bacterial phylogeny, host species and enrichment medium. In conclusion, this work depicts macroalgae as holobionts with an associated microbiota of interest for blue biotechnologies, suggesting isolation strategies and bacterial targets for polysaccharidases’ discoverySpringerSapientiaBarbato, MartaVacchini, ViolettaEngelen, AschwinPatania, GiovanniMapelli, FrancescaBorin, SaraCrotti, Elena2022-08-01T10:46:43Z2022-07-272022-08-01T03:13:20Z2022-07-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18135engAMB Express. 2022 Jul 27;12(1):9810.1186/s13568-022-01440-82191-0855info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:30:22Zoai:sapientia.ualg.pt:10400.1/18135Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:07:55.889296Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
title |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
spellingShingle |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria Barbato, Marta Blue biotechnology Epibiota Seaweeds Bioprospecting Cultivable microbiota Polysaccharidases |
title_short |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
title_full |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
title_fullStr |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
title_full_unstemmed |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
title_sort |
What lies on macroalgal surface: diversity of polysaccharide degraders in culturable epiphytic bacteria |
author |
Barbato, Marta |
author_facet |
Barbato, Marta Vacchini, Violetta Engelen, Aschwin Patania, Giovanni Mapelli, Francesca Borin, Sara Crotti, Elena |
author_role |
author |
author2 |
Vacchini, Violetta Engelen, Aschwin Patania, Giovanni Mapelli, Francesca Borin, Sara Crotti, Elena |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Barbato, Marta Vacchini, Violetta Engelen, Aschwin Patania, Giovanni Mapelli, Francesca Borin, Sara Crotti, Elena |
dc.subject.por.fl_str_mv |
Blue biotechnology Epibiota Seaweeds Bioprospecting Cultivable microbiota Polysaccharidases |
topic |
Blue biotechnology Epibiota Seaweeds Bioprospecting Cultivable microbiota Polysaccharidases |
description |
Macroalgal surface constitutes a peculiar ecological niche and an advantageous substratum for microorganisms able to degrade the wide diversity of algal glycans. The degrading enzymatic activities of macroalgal epiphytes are of paramount interest for the industrial by-product sector and biomass resource applications. We characterized the polysaccharide hydrolytic profle of bacterial isolates obtained from three macroalgal species: the red macroalgae Asparagopsis taxiformis and Sphaerococcus coronopifolius (Rhodophyceae) and the brown Halopteris scoparia (Phaeophyceae), sampled in South Portugal. Bacterial enrichment cultures supplemented with chlorinated aliphatic compounds, typically released by marine algae, were established using as inoculum the decaying biomass of the three macroalgae, obtaining a collection of 634 bacterial strains. Although collected from the same site and exposed to the same seawater seeding microbiota, macroalgal cultivable bacterial communities in terms of functional and phylogenetic diversity showed host specifcity. Isolates were tested for the hydrolysis of starch, pectin, alginate and agar, exhibiting a diferent hydrolytic potential according to their host: A. taxiformis showed the highest percentage of active isolates (91%), followed by S. coronopifolius (54%) and H. scoparia (46%). Only 30% of the isolates were able to degrade starch, while the other polymers were degraded by 55–58% of the isolates. Interestingly, several isolates showed promiscuous capacities to hydrolyze more than one polysaccharide. The isolate functional fngerprint was statistically correlated to bacterial phylogeny, host species and enrichment medium. In conclusion, this work depicts macroalgae as holobionts with an associated microbiota of interest for blue biotechnologies, suggesting isolation strategies and bacterial targets for polysaccharidases’ discovery |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01T10:46:43Z 2022-07-27 2022-08-01T03:13:20Z 2022-07-27T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/18135 |
url |
http://hdl.handle.net/10400.1/18135 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
AMB Express. 2022 Jul 27;12(1):98 10.1186/s13568-022-01440-8 2191-0855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133324511281152 |