Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.

Detalhes bibliográficos
Autor(a) principal: Pereira, Francisco
Data de Publicação: 2015
Outros Autores: Kong, Jian, Siva, Ana Luísa, Liebhaber, Stephen A, Romão, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3346
Resumo: Acessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513866/
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spelling Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.Expressão GénicaGenómica Funcional e EstruturalAcessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513866/Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). The level of sensitivity of a PTC-containing mRNA to NMD is multifactorial. We have previously shown that human β-globin mRNAs carrying PTCs in close proximity to the translation initiation AUG codon escape NMD. This was called the 'AUG-proximity effect'. The present analysis of nonsense codons in the human α-globin mRNA illustrates that the determinants of the AUG-proximity effect are in fact quite complex, reflecting the ability of the ribosome to re-initiate translation 3' to the PTC and the specific sequence and secondary structure of the translated ORF. These data support a model in which the time taken to translate the short ORF, impacted by distance, sequence, and structure, not only modulates translation re-initiation, but also impacts on the exact boundary of AUG-proximity protection from NMD.Oxford University PressRepositório Científico do Instituto Nacional de SaúdePereira, FranciscoKong, JianSiva, Ana LuísaLiebhaber, Stephen ARomão, Luísa2016-02-16T17:10:07Z2015-07-272015-07-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3346engNucleic Acids Res. 2015 Jul 27;43(13):6528-44. doi: 10.1093/nar/gkv588. Epub 2015 Jun 11.0305-104810.1093/nar/gkv588info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:46Zoai:repositorio.insa.pt:10400.18/3346Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:17.483892Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
title Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
spellingShingle Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
Pereira, Francisco
Expressão Génica
Genómica Funcional e Estrutural
title_short Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
title_full Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
title_fullStr Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
title_full_unstemmed Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
title_sort Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
author Pereira, Francisco
author_facet Pereira, Francisco
Kong, Jian
Siva, Ana Luísa
Liebhaber, Stephen A
Romão, Luísa
author_role author
author2 Kong, Jian
Siva, Ana Luísa
Liebhaber, Stephen A
Romão, Luísa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Pereira, Francisco
Kong, Jian
Siva, Ana Luísa
Liebhaber, Stephen A
Romão, Luísa
dc.subject.por.fl_str_mv Expressão Génica
Genómica Funcional e Estrutural
topic Expressão Génica
Genómica Funcional e Estrutural
description Acessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513866/
publishDate 2015
dc.date.none.fl_str_mv 2015-07-27
2015-07-27T00:00:00Z
2016-02-16T17:10:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3346
url http://hdl.handle.net/10400.18/3346
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nucleic Acids Res. 2015 Jul 27;43(13):6528-44. doi: 10.1093/nar/gkv588. Epub 2015 Jun 11.
0305-1048
10.1093/nar/gkv588
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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