Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1093/nar/gkv588 |
Resumo: | Fundacao para a Ciencia e a Tecnologia (FCT) [POCI/BIA-BCM/59140/2004 and UID/MULTI/04046/2013 to BioISI from FCT/MCTES/PIDDAC; SFRH/BD/14273/2003 to F.J.C.P. and SFRH/BD/63581/2009 to C.B.]. Funding for open access charge: Instituto Nacional de Saude Doutor Ricardo Jorge and Oxford Journals Publisher. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activityGeneticsFundacao para a Ciencia e a Tecnologia (FCT) [POCI/BIA-BCM/59140/2004 and UID/MULTI/04046/2013 to BioISI from FCT/MCTES/PIDDAC; SFRH/BD/14273/2003 to F.J.C.P. and SFRH/BD/63581/2009 to C.B.]. Funding for open access charge: Instituto Nacional de Saude Doutor Ricardo Jorge and Oxford Journals Publisher.Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). The level of sensitivity of a PTC-containing mRNA to NMD is multifactorial. We have previously shown that human β-globin mRNAs carrying PTCs in close proximity to the translation initiation AUG codon escape NMD. This was called the 'AUG-proximity effect'. The present analysis of nonsense codons in the human α-globin mRNA illustrates that the determinants of the AUG-proximity effect are in fact quite complex, reflecting the ability of the ribosome to re-initiate translation 3′ to the PTC and the specific sequence and secondary structure of the translated ORF. These data support a model in which the time taken to translate the short ORF, impacted by distance, sequence, and structure, not only modulates translation re-initiation, but also impacts on the exact boundary of AUG-proximity protection from NMD.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Investigação em Genética Molecular Humana (CIGMH)RUNPereira, Francisco J CTeixeira, Alexandre R. S.Kong, JianBarbosa, CristinaSilva, Ana LuísaMarques-Ramos, AnaLiebhaber, Stephen A.Romão, Luísa2017-10-17T22:02:35Z2015-05-232015-05-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttps://doi.org/10.1093/nar/gkv588eng0305-1048PURE: 3221531http://www.scopus.com/inward/record.url?scp=84939611842&partnerID=8YFLogxKhttps://doi.org/10.1093/nar/gkv588info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:12:35Zoai:run.unl.pt:10362/24286Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:28:01.530401Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| title |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| spellingShingle |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity Pereira, Francisco J C Genetics |
| title_short |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| title_full |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| title_fullStr |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| title_full_unstemmed |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| title_sort |
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity |
| author |
Pereira, Francisco J C |
| author_facet |
Pereira, Francisco J C Teixeira, Alexandre R. S. Kong, Jian Barbosa, Cristina Silva, Ana Luísa Marques-Ramos, Ana Liebhaber, Stephen A. Romão, Luísa |
| author_role |
author |
| author2 |
Teixeira, Alexandre R. S. Kong, Jian Barbosa, Cristina Silva, Ana Luísa Marques-Ramos, Ana Liebhaber, Stephen A. Romão, Luísa |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Investigação em Genética Molecular Humana (CIGMH) RUN |
| dc.contributor.author.fl_str_mv |
Pereira, Francisco J C Teixeira, Alexandre R. S. Kong, Jian Barbosa, Cristina Silva, Ana Luísa Marques-Ramos, Ana Liebhaber, Stephen A. Romão, Luísa |
| dc.subject.por.fl_str_mv |
Genetics |
| topic |
Genetics |
| description |
Fundacao para a Ciencia e a Tecnologia (FCT) [POCI/BIA-BCM/59140/2004 and UID/MULTI/04046/2013 to BioISI from FCT/MCTES/PIDDAC; SFRH/BD/14273/2003 to F.J.C.P. and SFRH/BD/63581/2009 to C.B.]. Funding for open access charge: Instituto Nacional de Saude Doutor Ricardo Jorge and Oxford Journals Publisher. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-05-23 2015-05-23T00:00:00Z 2017-10-17T22:02:35Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1093/nar/gkv588 |
| url |
https://doi.org/10.1093/nar/gkv588 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0305-1048 PURE: 3221531 http://www.scopus.com/inward/record.url?scp=84939611842&partnerID=8YFLogxK https://doi.org/10.1093/nar/gkv588 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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17 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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