Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells

Detalhes bibliográficos
Autor(a) principal: Ramalho, Patrícia A.
Data de Publicação: 2005
Outros Autores: Paiva, Sandra, Paulo, Artur Cavaco, Casal, Margarida, Cardoso, M. Helena, Ramalho, Maria Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2326
Resumo: In Press. Aceite em 2005 para publicação na revista Applied and Environmental Microbiology.
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spelling Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cellsIn Press. Aceite em 2005 para publicação na revista Applied and Environmental Microbiology.Unspecific bacterial reduction of azo dyes is a widely studied process in correlation with the biological treatment of coloured waste waters but the enzyme system associated to this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolourising activities. The yeast-mediated process requires an alternative carbon and energy source and is independent of previous exposure to the dyes. When substrate dyes are polar their reduction is extracellular, strongly suggesting the involvement of an externally-directed plasma membrane redox system. The present work demonstrates that, in Saccharomyces cerevisiae, the ferric reductase system participates in the extracellular reduction of azo dyes. The Saccharomyces cerevisiae mutant strains Δfre1 and Δfre1Δfre2, but not Δfre2, showed a much reduced decolourising capability, suggesting that, under the conditions tested, Fre1p is a major component of the azo reductase activity.European BIOEFTEX Project.Universidade do MinhoRamalho, Patrícia A.Paiva, SandraPaulo, Artur CavacoCasal, MargaridaCardoso, M. HelenaRamalho, Maria Teresa20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2326enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:30:32Zoai:repositorium.sdum.uminho.pt:1822/2326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:25:46.392468Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
title Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
spellingShingle Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
Ramalho, Patrícia A.
title_short Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
title_full Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
title_fullStr Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
title_full_unstemmed Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
title_sort Role of the component Fre1p of the plasma membrane ferric reductase on the azo reductase activity of intact Saccharomyces cerevisiae cells
author Ramalho, Patrícia A.
author_facet Ramalho, Patrícia A.
Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Cardoso, M. Helena
Ramalho, Maria Teresa
author_role author
author2 Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Cardoso, M. Helena
Ramalho, Maria Teresa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ramalho, Patrícia A.
Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Cardoso, M. Helena
Ramalho, Maria Teresa
description In Press. Aceite em 2005 para publicação na revista Applied and Environmental Microbiology.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
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