LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jpba.2017.08.036 http://hdl.handle.net/11449/159880 |
Resumo: | The ribonucleotide reductase inhibitor and radiosensitizer triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone (3-AP), NSC 663249) is clinically being evaluated via the intravenous (IV) route for the treatment of cervical and vulvar cancer in combination with primary cisplatin chemoradiation. The need for a 2-h infusion and frequent administration of triapine is logistically challenging, prompting us to pursue oral (PO) administration. In support of the clinical trial investigating oral triapine in combination with chemoradiation, we developed and validated a novel LC-MS/MS assay for the quantification of triapine in 50 mu L human plasma. After protein precipitation, chromatographic separation of the supernatant was achieved with a Shodex ODP2 column and an isocratic acetonitrile-water mobile phase with 10% ammonium acetate. Detection with an ABI 4000 mass spectrometer utilized electrospray positive mode ionization. The assay was linear from 3 to 3,000 ng/mL and proved to be accurate (97.1-103.1%) and precise (<7.4% CV), and met the U.S. FDA guidance for bioanalytical method validation. This LC-MS/MS assay will be an essential tool to further define the pharmacokinetics and oral bioavailability of triapine. (C) 2017 Elsevier B.V. All rights reserved. |
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LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasmaTriapineTandem mass spectrometryAssayValidationThe ribonucleotide reductase inhibitor and radiosensitizer triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone (3-AP), NSC 663249) is clinically being evaluated via the intravenous (IV) route for the treatment of cervical and vulvar cancer in combination with primary cisplatin chemoradiation. The need for a 2-h infusion and frequent administration of triapine is logistically challenging, prompting us to pursue oral (PO) administration. In support of the clinical trial investigating oral triapine in combination with chemoradiation, we developed and validated a novel LC-MS/MS assay for the quantification of triapine in 50 mu L human plasma. After protein precipitation, chromatographic separation of the supernatant was achieved with a Shodex ODP2 column and an isocratic acetonitrile-water mobile phase with 10% ammonium acetate. Detection with an ABI 4000 mass spectrometer utilized electrospray positive mode ionization. The assay was linear from 3 to 3,000 ng/mL and proved to be accurate (97.1-103.1%) and precise (<7.4% CV), and met the U.S. FDA guidance for bioanalytical method validation. This LC-MS/MS assay will be an essential tool to further define the pharmacokinetics and oral bioavailability of triapine. (C) 2017 Elsevier B.V. All rights reserved.NCI-CTEPNCIInstitute of International EducationConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Pittsburgh, Canc Therapeut Program, Canc Inst, Pittsburgh, PA 15213 USASao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP, BrazilUniv Pittsburgh, Magee Womens Hosp, Div Gynecol Oncol, Dept Obstet Gynecol & Reprod Med, Pittsburgh, PA 15213 USAUniv Pittsburgh, Sch Med, Dept Med, Div Hematol Oncol, Pittsburgh, PA 15213 USANCI, Investigat Drug Branch, Canc Therapy Evaluat Program, Div Canc Treatment & Diag, Bethesda, MD 20892 USAUniv Pittsburgh, Sch Pharm, Dept Pharmaceut Sci, Pittsburgh, PA 15213 USASao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP, BrazilNCI-CTEP: UM1-CA186690NCI: R50 CA211241: P30-CA47904Elsevier B.V.Univ PittsburghUniversidade Estadual Paulista (Unesp)NCIMatsumoto, Julia [UNESP]Kiesel, Brian F.Parise, Robert A.Guo, JianxiaTaylor, SarahHuang, MarilynEiseman, Julie L.Ivy, S. PercyKunos, CharlesChu, EdwardBeumer, Jan H.2018-11-26T15:45:35Z2018-11-26T15:45:35Z2017-11-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article154-160application/pdfhttp://dx.doi.org/10.1016/j.jpba.2017.08.036Journal Of Pharmaceutical And Biomedical Analysis. Amsterdam: Elsevier Science Bv, v. 146, p. 154-160, 2017.0731-7085http://hdl.handle.net/11449/15988010.1016/j.jpba.2017.08.036WOS:000413283400020WOS000413283400020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Pharmaceutical And Biomedical Analysis0,919info:eu-repo/semantics/openAccess2023-12-24T06:18:36Zoai:repositorio.unesp.br:11449/159880Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:10:44.239722Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
title |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
spellingShingle |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma Matsumoto, Julia [UNESP] Triapine Tandem mass spectrometry Assay Validation |
title_short |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
title_full |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
title_fullStr |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
title_full_unstemmed |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
title_sort |
LC-MS/MS assay for the quantitation of the ribonucleotide reductase inhibitor triapine in human plasma |
author |
Matsumoto, Julia [UNESP] |
author_facet |
Matsumoto, Julia [UNESP] Kiesel, Brian F. Parise, Robert A. Guo, Jianxia Taylor, Sarah Huang, Marilyn Eiseman, Julie L. Ivy, S. Percy Kunos, Charles Chu, Edward Beumer, Jan H. |
author_role |
author |
author2 |
Kiesel, Brian F. Parise, Robert A. Guo, Jianxia Taylor, Sarah Huang, Marilyn Eiseman, Julie L. Ivy, S. Percy Kunos, Charles Chu, Edward Beumer, Jan H. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Pittsburgh Universidade Estadual Paulista (Unesp) NCI |
dc.contributor.author.fl_str_mv |
Matsumoto, Julia [UNESP] Kiesel, Brian F. Parise, Robert A. Guo, Jianxia Taylor, Sarah Huang, Marilyn Eiseman, Julie L. Ivy, S. Percy Kunos, Charles Chu, Edward Beumer, Jan H. |
dc.subject.por.fl_str_mv |
Triapine Tandem mass spectrometry Assay Validation |
topic |
Triapine Tandem mass spectrometry Assay Validation |
description |
The ribonucleotide reductase inhibitor and radiosensitizer triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone (3-AP), NSC 663249) is clinically being evaluated via the intravenous (IV) route for the treatment of cervical and vulvar cancer in combination with primary cisplatin chemoradiation. The need for a 2-h infusion and frequent administration of triapine is logistically challenging, prompting us to pursue oral (PO) administration. In support of the clinical trial investigating oral triapine in combination with chemoradiation, we developed and validated a novel LC-MS/MS assay for the quantification of triapine in 50 mu L human plasma. After protein precipitation, chromatographic separation of the supernatant was achieved with a Shodex ODP2 column and an isocratic acetonitrile-water mobile phase with 10% ammonium acetate. Detection with an ABI 4000 mass spectrometer utilized electrospray positive mode ionization. The assay was linear from 3 to 3,000 ng/mL and proved to be accurate (97.1-103.1%) and precise (<7.4% CV), and met the U.S. FDA guidance for bioanalytical method validation. This LC-MS/MS assay will be an essential tool to further define the pharmacokinetics and oral bioavailability of triapine. (C) 2017 Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-30 2018-11-26T15:45:35Z 2018-11-26T15:45:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jpba.2017.08.036 Journal Of Pharmaceutical And Biomedical Analysis. Amsterdam: Elsevier Science Bv, v. 146, p. 154-160, 2017. 0731-7085 http://hdl.handle.net/11449/159880 10.1016/j.jpba.2017.08.036 WOS:000413283400020 WOS000413283400020.pdf |
url |
http://dx.doi.org/10.1016/j.jpba.2017.08.036 http://hdl.handle.net/11449/159880 |
identifier_str_mv |
Journal Of Pharmaceutical And Biomedical Analysis. Amsterdam: Elsevier Science Bv, v. 146, p. 154-160, 2017. 0731-7085 10.1016/j.jpba.2017.08.036 WOS:000413283400020 WOS000413283400020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Pharmaceutical And Biomedical Analysis 0,919 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
154-160 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129294628552704 |