Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/100825 |
Resumo: | During a survey of yeast populations associated with grape and apple musts used for wine and cider fermentation, respectively, six pink-coloured ballistoconidia-forming yeasts belonging to the order Sporidiobolales (Basidiomycota) were isolated. Phylogenetic analysis inferred using sequences of the internal transcribed spacer (ITS), the D1/D2 domain of the large subunit rRNA gene, the small subunit (SSU) rRNA gene and DNA-directed RNA polymerase II subunit (RPB2) indicated that the six isolates were separated in two novel species. One of the new species, Sporobolomyces agrorum sp. nov., isolated from grape must, had Sporobolomyces roseus and Sporobolomyces metaroseus as its closest relatives, but showed four/two and 16 nucleotide substitutions in the D1/D2 and ITS regions, respectively, to these two species. The other novel species, Sporobolomyces sucorum sp. nov., was found in apple must and was closely related to Sporobolomyces pararoseus and Sporobolomyces patagonicus, but showed two/three and five substitutions in those two regions for its closest relatives. We detected additional representatives of this species, most of them isolated from grapes whose sequences were already available on public databases. A sexual stage could not be observed for the novel species. |
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Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italyApple juiceBasidiomycetous yeastGrape mustSporobolomyces agrorumSporobolomyces sucorumTaxonomyMicrobiologyEcology, Evolution, Behavior and SystematicsDuring a survey of yeast populations associated with grape and apple musts used for wine and cider fermentation, respectively, six pink-coloured ballistoconidia-forming yeasts belonging to the order Sporidiobolales (Basidiomycota) were isolated. Phylogenetic analysis inferred using sequences of the internal transcribed spacer (ITS), the D1/D2 domain of the large subunit rRNA gene, the small subunit (SSU) rRNA gene and DNA-directed RNA polymerase II subunit (RPB2) indicated that the six isolates were separated in two novel species. One of the new species, Sporobolomyces agrorum sp. nov., isolated from grape must, had Sporobolomyces roseus and Sporobolomyces metaroseus as its closest relatives, but showed four/two and 16 nucleotide substitutions in the D1/D2 and ITS regions, respectively, to these two species. The other novel species, Sporobolomyces sucorum sp. nov., was found in apple must and was closely related to Sporobolomyces pararoseus and Sporobolomyces patagonicus, but showed two/three and five substitutions in those two regions for its closest relatives. We detected additional representatives of this species, most of them isolated from grapes whose sequences were already available on public databases. A sexual stage could not be observed for the novel species.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNLorenzini, MarilindaZapparoli, GiacomoAzzolini, MichelaCarvalho, ClaudiaSampaio, Jose Paulo2020-07-13T22:48:09Z2019-11-012019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/100825eng1466-5026PURE: 18999416https://doi.org/10.1099/ijsem.0.003626info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:47:09Zoai:run.unl.pt:10362/100825Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:39:25.878674Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
title |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
spellingShingle |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy Lorenzini, Marilinda Apple juice Basidiomycetous yeast Grape must Sporobolomyces agrorum Sporobolomyces sucorum Taxonomy Microbiology Ecology, Evolution, Behavior and Systematics |
title_short |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
title_full |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
title_fullStr |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
title_full_unstemmed |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
title_sort |
Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy |
author |
Lorenzini, Marilinda |
author_facet |
Lorenzini, Marilinda Zapparoli, Giacomo Azzolini, Michela Carvalho, Claudia Sampaio, Jose Paulo |
author_role |
author |
author2 |
Zapparoli, Giacomo Azzolini, Michela Carvalho, Claudia Sampaio, Jose Paulo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit RUN |
dc.contributor.author.fl_str_mv |
Lorenzini, Marilinda Zapparoli, Giacomo Azzolini, Michela Carvalho, Claudia Sampaio, Jose Paulo |
dc.subject.por.fl_str_mv |
Apple juice Basidiomycetous yeast Grape must Sporobolomyces agrorum Sporobolomyces sucorum Taxonomy Microbiology Ecology, Evolution, Behavior and Systematics |
topic |
Apple juice Basidiomycetous yeast Grape must Sporobolomyces agrorum Sporobolomyces sucorum Taxonomy Microbiology Ecology, Evolution, Behavior and Systematics |
description |
During a survey of yeast populations associated with grape and apple musts used for wine and cider fermentation, respectively, six pink-coloured ballistoconidia-forming yeasts belonging to the order Sporidiobolales (Basidiomycota) were isolated. Phylogenetic analysis inferred using sequences of the internal transcribed spacer (ITS), the D1/D2 domain of the large subunit rRNA gene, the small subunit (SSU) rRNA gene and DNA-directed RNA polymerase II subunit (RPB2) indicated that the six isolates were separated in two novel species. One of the new species, Sporobolomyces agrorum sp. nov., isolated from grape must, had Sporobolomyces roseus and Sporobolomyces metaroseus as its closest relatives, but showed four/two and 16 nucleotide substitutions in the D1/D2 and ITS regions, respectively, to these two species. The other novel species, Sporobolomyces sucorum sp. nov., was found in apple must and was closely related to Sporobolomyces pararoseus and Sporobolomyces patagonicus, but showed two/three and five substitutions in those two regions for its closest relatives. We detected additional representatives of this species, most of them isolated from grapes whose sequences were already available on public databases. A sexual stage could not be observed for the novel species. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2019-11-01T00:00:00Z 2020-07-13T22:48:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/100825 |
url |
http://hdl.handle.net/10362/100825 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1466-5026 PURE: 18999416 https://doi.org/10.1099/ijsem.0.003626 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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