West Nile virus capsid protein interacts with biologically relevant host lipid systems

Bibliographic Details
Main Author: Martins, Ana S.
Publication Date: 2019
Other Authors: Carvalho, Filomena Almeida, Faustino, André F., Martins, Ivo C., Santos, Nuno C.
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: http://hdl.handle.net/10451/37841
Summary: Copyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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spelling West Nile virus capsid protein interacts with biologically relevant host lipid systemsWest Nile virusLipid dropletsLipoproteinsAtomic force microscopyForce spectroscopyDynamic light scatteringZeta potentialCopyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.West Nile and dengue viruses are closely related flaviviruses, originating mosquito-borne viral infections for which there are no effective and specific treatments. Their capsid proteins sequence and structure are particularly similar, forming highly superimposable α-helical homodimers. Measuring protein-ligand interactions at the single-molecule level yields detailed information of biological and biomedical relevance. In this work, such an approach was successfully applied on the characterization of the West Nile virus capsid protein interaction with host lipid systems, namely intracellular lipid droplets (an essential step for dengue virus replication) and blood plasma lipoproteins. Dynamic light scattering measurements show that West Nile virus capsid protein binds very low-density lipoproteins, but not low-density lipoproteins, and this interaction is dependent of potassium ions. Zeta potential experiments show that the interaction with lipid droplets is also dependent of potassium ions as well as surface proteins. The forces involved on the binding of the capsid protein with lipid droplets and lipoproteins were determined using atomic force microscopy-based force spectroscopy, proving that these interactions are K+-dependent rather than a general dependence of ionic strength. The capsid protein interaction with host lipid systems may be targeted in future therapeutic strategies against different flaviviruses. The biophysical and nanotechnology approaches employed in this study may be applied to characterize the interactions of other important proteins from different viruses, in order to understand their life cycles, as well as to find new strategies to inhibit them.This work was supported by Fundação para a Ciência e a Tecnologia–Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/SAUENB/117013/2010. AF and AM also acknowledge FCT-MCTES fellowships SFRH/BD/77609/2011 and PD/BD/113698/2015, respectively. IM acknowledges consecutive funding from the FCT-MCTES fellowship SFRH/BPD/74287/2010 and the Program Investigador FCT (Research Contract IF/00772/2013). This work was also supported by UID/BIM/50005/2019, project funded by FCT-MCTES through Fundos do Orçamento do Estado.Frontiers MediaRepositório da Universidade de LisboaMartins, Ana S.Carvalho, Filomena AlmeidaFaustino, André F.Martins, Ivo C.Santos, Nuno C.2019-04-03T15:27:30Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/37841engFront. Cell. Infect. Microbiol., 06 February 2019 | Volume 9 | Article 82235-298810.3389/fcimb.2019.00008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:35:14Zoai:repositorio.ul.pt:10451/37841Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:51:48.983591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv West Nile virus capsid protein interacts with biologically relevant host lipid systems
title West Nile virus capsid protein interacts with biologically relevant host lipid systems
spellingShingle West Nile virus capsid protein interacts with biologically relevant host lipid systems
Martins, Ana S.
West Nile virus
Lipid droplets
Lipoproteins
Atomic force microscopy
Force spectroscopy
Dynamic light scattering
Zeta potential
title_short West Nile virus capsid protein interacts with biologically relevant host lipid systems
title_full West Nile virus capsid protein interacts with biologically relevant host lipid systems
title_fullStr West Nile virus capsid protein interacts with biologically relevant host lipid systems
title_full_unstemmed West Nile virus capsid protein interacts with biologically relevant host lipid systems
title_sort West Nile virus capsid protein interacts with biologically relevant host lipid systems
author Martins, Ana S.
author_facet Martins, Ana S.
Carvalho, Filomena Almeida
Faustino, André F.
Martins, Ivo C.
Santos, Nuno C.
author_role author
author2 Carvalho, Filomena Almeida
Faustino, André F.
Martins, Ivo C.
Santos, Nuno C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Martins, Ana S.
Carvalho, Filomena Almeida
Faustino, André F.
Martins, Ivo C.
Santos, Nuno C.
dc.subject.por.fl_str_mv West Nile virus
Lipid droplets
Lipoproteins
Atomic force microscopy
Force spectroscopy
Dynamic light scattering
Zeta potential
topic West Nile virus
Lipid droplets
Lipoproteins
Atomic force microscopy
Force spectroscopy
Dynamic light scattering
Zeta potential
description Copyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-03T15:27:30Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/37841
url http://hdl.handle.net/10451/37841
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front. Cell. Infect. Microbiol., 06 February 2019 | Volume 9 | Article 8
2235-2988
10.3389/fcimb.2019.00008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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