West Nile virus capsid protein interacts with biologically relevant host lipid systems
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/37841 |
Resumo: | Copyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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West Nile virus capsid protein interacts with biologically relevant host lipid systemsWest Nile virusLipid dropletsLipoproteinsAtomic force microscopyForce spectroscopyDynamic light scatteringZeta potentialCopyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.West Nile and dengue viruses are closely related flaviviruses, originating mosquito-borne viral infections for which there are no effective and specific treatments. Their capsid proteins sequence and structure are particularly similar, forming highly superimposable α-helical homodimers. Measuring protein-ligand interactions at the single-molecule level yields detailed information of biological and biomedical relevance. In this work, such an approach was successfully applied on the characterization of the West Nile virus capsid protein interaction with host lipid systems, namely intracellular lipid droplets (an essential step for dengue virus replication) and blood plasma lipoproteins. Dynamic light scattering measurements show that West Nile virus capsid protein binds very low-density lipoproteins, but not low-density lipoproteins, and this interaction is dependent of potassium ions. Zeta potential experiments show that the interaction with lipid droplets is also dependent of potassium ions as well as surface proteins. The forces involved on the binding of the capsid protein with lipid droplets and lipoproteins were determined using atomic force microscopy-based force spectroscopy, proving that these interactions are K+-dependent rather than a general dependence of ionic strength. The capsid protein interaction with host lipid systems may be targeted in future therapeutic strategies against different flaviviruses. The biophysical and nanotechnology approaches employed in this study may be applied to characterize the interactions of other important proteins from different viruses, in order to understand their life cycles, as well as to find new strategies to inhibit them.This work was supported by Fundação para a Ciência e a Tecnologia–Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/SAUENB/117013/2010. AF and AM also acknowledge FCT-MCTES fellowships SFRH/BD/77609/2011 and PD/BD/113698/2015, respectively. IM acknowledges consecutive funding from the FCT-MCTES fellowship SFRH/BPD/74287/2010 and the Program Investigador FCT (Research Contract IF/00772/2013). This work was also supported by UID/BIM/50005/2019, project funded by FCT-MCTES through Fundos do Orçamento do Estado.Frontiers MediaRepositório da Universidade de LisboaMartins, Ana S.Carvalho, Filomena AlmeidaFaustino, André F.Martins, Ivo C.Santos, Nuno C.2019-04-03T15:27:30Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/37841engFront. Cell. Infect. Microbiol., 06 February 2019 | Volume 9 | Article 82235-298810.3389/fcimb.2019.00008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:35:14Zoai:repositorio.ul.pt:10451/37841Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:51:48.983591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| title |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| spellingShingle |
West Nile virus capsid protein interacts with biologically relevant host lipid systems Martins, Ana S. West Nile virus Lipid droplets Lipoproteins Atomic force microscopy Force spectroscopy Dynamic light scattering Zeta potential |
| title_short |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| title_full |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| title_fullStr |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| title_full_unstemmed |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| title_sort |
West Nile virus capsid protein interacts with biologically relevant host lipid systems |
| author |
Martins, Ana S. |
| author_facet |
Martins, Ana S. Carvalho, Filomena Almeida Faustino, André F. Martins, Ivo C. Santos, Nuno C. |
| author_role |
author |
| author2 |
Carvalho, Filomena Almeida Faustino, André F. Martins, Ivo C. Santos, Nuno C. |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Martins, Ana S. Carvalho, Filomena Almeida Faustino, André F. Martins, Ivo C. Santos, Nuno C. |
| dc.subject.por.fl_str_mv |
West Nile virus Lipid droplets Lipoproteins Atomic force microscopy Force spectroscopy Dynamic light scattering Zeta potential |
| topic |
West Nile virus Lipid droplets Lipoproteins Atomic force microscopy Force spectroscopy Dynamic light scattering Zeta potential |
| description |
Copyright © 2019 Martins, Carvalho, Faustino, Martins and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-04-03T15:27:30Z 2019 2019-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/37841 |
| url |
http://hdl.handle.net/10451/37841 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Front. Cell. Infect. Microbiol., 06 February 2019 | Volume 9 | Article 8 2235-2988 10.3389/fcimb.2019.00008 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Frontiers Media |
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Frontiers Media |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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