Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration

Detalhes bibliográficos
Autor(a) principal: Bravo-Ferrada, B. M.
Data de Publicação: 2015
Outros Autores: Gonçalves, S., Semorile, L., Santos, N. C., Tymczyszyn, E. E., Hollmann, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23602
Resumo: © 2015 The Society for Applied Microbiology
id RCAP_90c4f4da643d4abd2aa04ed3b16ba7c2
oai_identifier_str oai:repositorio.ul.pt:10451/23602
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydrationAtomic force microscopyFreeze-dryingLactobacillus plantarumWineZeta potential© 2015 The Society for Applied MicrobiologyAims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.This work has been funded by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicaciones Biotecnológicas, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT – MINCyT, Argentina; PICTO-UNQ 2006 N-36474 and PICT SU 2012 N° 2804), Comisión Nacional de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina) and Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCTMEC, Portugal). Bravo-Ferrada, B.M. has a fellowship from the Consejo Nacional de Investigaciones Científicasy Técnicas (CONICET). AH and EET are members of the Carrera del Investigador Científico y Tecnológico (CONICET, Argentina). LS is member of the Research Career of Comisión de Investigaciones Científicas de la Provinciade Buenos Aires (CIC-BA).WileyRepositório da Universidade de LisboaBravo-Ferrada, B. M.Gonçalves, S.Semorile, L.Santos, N. C.Tymczyszyn, E. E.Hollmann, A.2016-05-04T15:32:41Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23602engJournal of Applied Microbiology 118, 1409-14171364-507210.1111/jam.12796metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:11:43Zoai:repositorio.ul.pt:10451/23602Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:55.278622Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
spellingShingle Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
Bravo-Ferrada, B. M.
Atomic force microscopy
Freeze-drying
Lactobacillus plantarum
Wine
Zeta potential
title_short Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_full Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_fullStr Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_full_unstemmed Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_sort Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
author Bravo-Ferrada, B. M.
author_facet Bravo-Ferrada, B. M.
Gonçalves, S.
Semorile, L.
Santos, N. C.
Tymczyszyn, E. E.
Hollmann, A.
author_role author
author2 Gonçalves, S.
Semorile, L.
Santos, N. C.
Tymczyszyn, E. E.
Hollmann, A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Bravo-Ferrada, B. M.
Gonçalves, S.
Semorile, L.
Santos, N. C.
Tymczyszyn, E. E.
Hollmann, A.
dc.subject.por.fl_str_mv Atomic force microscopy
Freeze-drying
Lactobacillus plantarum
Wine
Zeta potential
topic Atomic force microscopy
Freeze-drying
Lactobacillus plantarum
Wine
Zeta potential
description © 2015 The Society for Applied Microbiology
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-05-04T15:32:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23602
url http://hdl.handle.net/10451/23602
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Microbiology 118, 1409-1417
1364-5072
10.1111/jam.12796
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134320007315456

Registros relacionados