Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/52327 |
Resumo: | For a long time Helicobacter pylori infections have been treated using the macrolide antibiotic, clarithromycin. Clarithromycin resistance is increasing worldwide and is the most common cause of H. pylori treatment failure. Here we review the mechanisms of antibiotic resistance to clarithromycin, detailing the individual and combinations of point mutations found in the 23S rRNA gene associated with resistance. Additionally, we consider the methods used to detect clarithromycin resistance, emphasizing the use of high-throughput next-generation sequencing methods, which were applied to 17 newly sequenced pairs of H. pylori strains isolated from the antrum and corpus of a recent colonized paediatric population. This set of isolates was composed of six pairs of resistant strains whose phenotype was associated with two point mutations found in the 23S rRNA gene: A2142C and A2143G. Other point mutations were found simultaneously in the same gene, but, according to our results, it is unlikely that they contribute to resistance. Further, among susceptible isolates, genomic variations compatible with mutations previously associated with clarithromycin resistance were detected. Exposure to clarithromycin may select low-frequency variants, resulting in a progressive increase in the resistance rate due to selection pressure. |
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Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approachesHelicobacter pyloriclarithromycinresistance23S ribosomal RNA subunitnext-generation sequencingpoint mutationsFor a long time Helicobacter pylori infections have been treated using the macrolide antibiotic, clarithromycin. Clarithromycin resistance is increasing worldwide and is the most common cause of H. pylori treatment failure. Here we review the mechanisms of antibiotic resistance to clarithromycin, detailing the individual and combinations of point mutations found in the 23S rRNA gene associated with resistance. Additionally, we consider the methods used to detect clarithromycin resistance, emphasizing the use of high-throughput next-generation sequencing methods, which were applied to 17 newly sequenced pairs of H. pylori strains isolated from the antrum and corpus of a recent colonized paediatric population. This set of isolates was composed of six pairs of resistant strains whose phenotype was associated with two point mutations found in the 23S rRNA gene: A2142C and A2143G. Other point mutations were found simultaneously in the same gene, but, according to our results, it is unlikely that they contribute to resistance. Further, among susceptible isolates, genomic variations compatible with mutations previously associated with clarithromycin resistance were detected. Exposure to clarithromycin may select low-frequency variants, resulting in a progressive increase in the resistance rate due to selection pressure.F. F. V. is the recipient of a project grant (PTDC/BTM-SAL/28978/2017) from the Fundação para a Ciência e a Tecnologia (FCT), which supported this work. J. V.’s research group was financed by New England Biolabs, Inc. (USA).Microbiology Society [Society Publisher]Repositório da Universidade de LisboaMarques, Andreia T.Vítor, Jorge M. B.Santos, AndreaOleastro, MónicaVale, Filipa2022-04-13T11:05:52Z2020-03-022022-02-24T14:59:58Z2020-03-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/52327engMarques AT, Vítor JMB, Santos A, Oleastro M, Vale FF. Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches. Microbial Genomics [Internet]. 2020;6(3). Disponível em: https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.0003442057-5858cv-prod-1216082https://doi.org/10.1099/mgen.0.000344info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:14Zoai:repositorio.ul.pt:10451/52327Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:47.151152Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
title |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
spellingShingle |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches Marques, Andreia T. Helicobacter pylori clarithromycin resistance 23S ribosomal RNA subunit next-generation sequencing point mutations |
title_short |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
title_full |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
title_fullStr |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
title_full_unstemmed |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
title_sort |
Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches |
author |
Marques, Andreia T. |
author_facet |
Marques, Andreia T. Vítor, Jorge M. B. Santos, Andrea Oleastro, Mónica Vale, Filipa |
author_role |
author |
author2 |
Vítor, Jorge M. B. Santos, Andrea Oleastro, Mónica Vale, Filipa |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Marques, Andreia T. Vítor, Jorge M. B. Santos, Andrea Oleastro, Mónica Vale, Filipa |
dc.subject.por.fl_str_mv |
Helicobacter pylori clarithromycin resistance 23S ribosomal RNA subunit next-generation sequencing point mutations |
topic |
Helicobacter pylori clarithromycin resistance 23S ribosomal RNA subunit next-generation sequencing point mutations |
description |
For a long time Helicobacter pylori infections have been treated using the macrolide antibiotic, clarithromycin. Clarithromycin resistance is increasing worldwide and is the most common cause of H. pylori treatment failure. Here we review the mechanisms of antibiotic resistance to clarithromycin, detailing the individual and combinations of point mutations found in the 23S rRNA gene associated with resistance. Additionally, we consider the methods used to detect clarithromycin resistance, emphasizing the use of high-throughput next-generation sequencing methods, which were applied to 17 newly sequenced pairs of H. pylori strains isolated from the antrum and corpus of a recent colonized paediatric population. This set of isolates was composed of six pairs of resistant strains whose phenotype was associated with two point mutations found in the 23S rRNA gene: A2142C and A2143G. Other point mutations were found simultaneously in the same gene, but, according to our results, it is unlikely that they contribute to resistance. Further, among susceptible isolates, genomic variations compatible with mutations previously associated with clarithromycin resistance were detected. Exposure to clarithromycin may select low-frequency variants, resulting in a progressive increase in the resistance rate due to selection pressure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-02 2020-03-02T00:00:00Z 2022-04-13T11:05:52Z 2022-02-24T14:59:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/52327 |
url |
http://hdl.handle.net/10451/52327 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marques AT, Vítor JMB, Santos A, Oleastro M, Vale FF. Trends in Helicobacter pylori resistance to clarithromycin: from phenotypic to genomic approaches. Microbial Genomics [Internet]. 2020;6(3). Disponível em: https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000344 2057-5858 cv-prod-1216082 https://doi.org/10.1099/mgen.0.000344 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Microbiology Society [Society Publisher] |
publisher.none.fl_str_mv |
Microbiology Society [Society Publisher] |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134578033557504 |