Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum

• Autor(a) principal: Queiroz, Marisa Vieira de
• Data de Publicação: 2007
• Outros Autores: Lana, Taís Guimarães, Gonçalves, Daniel Bonoto, Araújo, Elza Fernandes de, Brito, Admilson Toscano Ribeiro de, Varavallo, Maurilio Antonio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1590/S1517-83822007000100011; https://locus.ufv.br//handle/123456789/27167
• topic: Protoplast fusion; Pectinases; Penicillium; Fusão de protoplastos

Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold) and pectin lyase production (1.2-fold).