Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex
| Autor(a) principal: | |
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| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/127576 |
Resumo: | Funding Information: Funding: This work was supported by Fundação para Ciência e Tecnologia [PTDC/MED-TOX/30418/2017] and iNOVA4Health [UID/Multi/04462/2013]. M.J.C., F.L.-C., N.R.C., C.G.-D. and J.M. are supported by FCT grants [SFRH/BD/131331/2017, PD/BD/128337/2017, PD/BD/114257/2016, and PD/BD/105892/2014, PTDC/MED-TOX/30418/2017 respectively]. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortexAnimal modelsArterial hypertensionCYP1A1CystineEndothelial dysfunctionNon-radical oxidative speciesObstructive sleep apneaPrecision medicineThiolsXCTBiochemistryPhysiologyMolecular BiologyClinical BiochemistryCell BiologySDG 3 - Good Health and Well-beingFunding Information: Funding: This work was supported by Fundação para Ciência e Tecnologia [PTDC/MED-TOX/30418/2017] and iNOVA4Health [UID/Multi/04462/2013]. M.J.C., F.L.-C., N.R.C., C.G.-D. and J.M. are supported by FCT grants [SFRH/BD/131331/2017, PD/BD/128337/2017, PD/BD/114257/2016, and PD/BD/105892/2014, PTDC/MED-TOX/30418/2017 respectively]. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.We hypothesized that an interplay between aryl hydrocarbon receptor (AhR) and cysteine-related thiolome at the kidney cortex underlies the mechanisms of (mal)adaptation to chronic intermittent hypoxia (CIH), promoting arterial hypertension (HTN). Using a rat model of CIH-HTN, we investigated the impact of short-term (1 and 7 days), mid-term (14 and 21 days, pre-HTN), and long-term intermittent hypoxia (IH) (up to 60 days, established HTN) on Cyp1a1 protein level (a sensitive hallmark of AhR activation) and cysteine-related thiol pools. We found that acute and chronic IH had opposite effects on Cyp1a1 and the thiolome. While short-term IH decreased Cyp1a1 and increased protein-S-thiolation, long-term IH increased Cyp1a1 and free oxidized cysteine. In addition, an in vitro administration of cystine, but not cysteine, to human endothelial cells increased Cyp1a1 expression, supporting cystine as a putative AhR activator. This study supports Cyp1a1 as a biomarker of obstructive sleep apnea (OSA) severity and oxidized pools of cysteine as risk indicator of OSA-HTN. This work contributes to a better understanding of the mechanisms underlying the phenotype of OSA-HTN, mimicked by this model, which is in line with precision medicine challenges in OSA.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCorreia, Maria JoãoPimpão, António B.Lopes-Coelho, FilipaSequeira, Catarina O.Coelho, Nuno R.Gonçalves-Dias, ClaraBarouki, RobertCoumoul, XavierSerpa, JacintaMorello, JuditMonteiro, Emília C.Pereira, Sofia A.2021-11-11T23:38:43Z2021-092021-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/127576eng2076-3921PURE: 33949785https://doi.org/10.3390/antiox10091484info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:07:31Zoai:run.unl.pt:10362/127576Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:09.411608Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| title |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| spellingShingle |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex Correia, Maria João Animal models Arterial hypertension CYP1A1 Cystine Endothelial dysfunction Non-radical oxidative species Obstructive sleep apnea Precision medicine Thiols XCT Biochemistry Physiology Molecular Biology Clinical Biochemistry Cell Biology SDG 3 - Good Health and Well-being |
| title_short |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| title_full |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| title_fullStr |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| title_full_unstemmed |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| title_sort |
Aryl hydrocarbon receptor and cysteine redox dynamics underlie (Mal)adaptive mechanisms to chronic intermittent hypoxia in kidney cortex |
| author |
Correia, Maria João |
| author_facet |
Correia, Maria João Pimpão, António B. Lopes-Coelho, Filipa Sequeira, Catarina O. Coelho, Nuno R. Gonçalves-Dias, Clara Barouki, Robert Coumoul, Xavier Serpa, Jacinta Morello, Judit Monteiro, Emília C. Pereira, Sofia A. |
| author_role |
author |
| author2 |
Pimpão, António B. Lopes-Coelho, Filipa Sequeira, Catarina O. Coelho, Nuno R. Gonçalves-Dias, Clara Barouki, Robert Coumoul, Xavier Serpa, Jacinta Morello, Judit Monteiro, Emília C. Pereira, Sofia A. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Correia, Maria João Pimpão, António B. Lopes-Coelho, Filipa Sequeira, Catarina O. Coelho, Nuno R. Gonçalves-Dias, Clara Barouki, Robert Coumoul, Xavier Serpa, Jacinta Morello, Judit Monteiro, Emília C. Pereira, Sofia A. |
| dc.subject.por.fl_str_mv |
Animal models Arterial hypertension CYP1A1 Cystine Endothelial dysfunction Non-radical oxidative species Obstructive sleep apnea Precision medicine Thiols XCT Biochemistry Physiology Molecular Biology Clinical Biochemistry Cell Biology SDG 3 - Good Health and Well-being |
| topic |
Animal models Arterial hypertension CYP1A1 Cystine Endothelial dysfunction Non-radical oxidative species Obstructive sleep apnea Precision medicine Thiols XCT Biochemistry Physiology Molecular Biology Clinical Biochemistry Cell Biology SDG 3 - Good Health and Well-being |
| description |
Funding Information: Funding: This work was supported by Fundação para Ciência e Tecnologia [PTDC/MED-TOX/30418/2017] and iNOVA4Health [UID/Multi/04462/2013]. M.J.C., F.L.-C., N.R.C., C.G.-D. and J.M. are supported by FCT grants [SFRH/BD/131331/2017, PD/BD/128337/2017, PD/BD/114257/2016, and PD/BD/105892/2014, PTDC/MED-TOX/30418/2017 respectively]. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-11-11T23:38:43Z 2021-09 2021-09-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/127576 |
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http://hdl.handle.net/10362/127576 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2076-3921 PURE: 33949785 https://doi.org/10.3390/antiox10091484 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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