Dissecting mitosis by RNAi in Drosophila tissue culture cells

Detalhes bibliográficos
Autor(a) principal: Maiato, H
Data de Publicação: 2003
Outros Autores: Sunkel, CE, Earnshaw, WC
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10216/35063
Resumo: interference (RNAi) in cultured cells of Drosophila melanogaster. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST/Orbit as an example for the usefulness of the technique.
id RCAP_83b3e2c00da8f05bda16392734ca3f27
oai_identifier_str oai:repositorio-aberto.up.pt:10216/35063
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Dissecting mitosis by RNAi in Drosophila tissue culture cellsRNAiMitosisMastOrbitMicrotubulesMAPKinetochoresSpindleDrosophilainterference (RNAi) in cultured cells of Drosophila melanogaster. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST/Orbit as an example for the usefulness of the technique.20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/35063eng1480-9222Maiato, HSunkel, CEEarnshaw, WCinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T16:14:13Zoai:repositorio-aberto.up.pt:10216/35063Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:39:35.520125Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dissecting mitosis by RNAi in Drosophila tissue culture cells
title Dissecting mitosis by RNAi in Drosophila tissue culture cells
spellingShingle Dissecting mitosis by RNAi in Drosophila tissue culture cells
Maiato, H
RNAi
Mitosis
Mast
Orbit
Microtubules
MAP
Kinetochores
Spindle
Drosophila
title_short Dissecting mitosis by RNAi in Drosophila tissue culture cells
title_full Dissecting mitosis by RNAi in Drosophila tissue culture cells
title_fullStr Dissecting mitosis by RNAi in Drosophila tissue culture cells
title_full_unstemmed Dissecting mitosis by RNAi in Drosophila tissue culture cells
title_sort Dissecting mitosis by RNAi in Drosophila tissue culture cells
author Maiato, H
author_facet Maiato, H
Sunkel, CE
Earnshaw, WC
author_role author
author2 Sunkel, CE
Earnshaw, WC
author2_role author
author
dc.contributor.author.fl_str_mv Maiato, H
Sunkel, CE
Earnshaw, WC
dc.subject.por.fl_str_mv RNAi
Mitosis
Mast
Orbit
Microtubules
MAP
Kinetochores
Spindle
Drosophila
topic RNAi
Mitosis
Mast
Orbit
Microtubules
MAP
Kinetochores
Spindle
Drosophila
description interference (RNAi) in cultured cells of Drosophila melanogaster. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST/Orbit as an example for the usefulness of the technique.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10216/35063
url http://hdl.handle.net/10216/35063
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1480-9222
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136302432518144

Registros relacionados