Biofilms formed on humic substances : response to flow conditions and carbon concentrations

Detalhes bibliográficos
Autor(a) principal: Rodrigues, A. L.
Data de Publicação: 2010
Outros Autores: Pereira, M. A., Janknecht, P., Brito, A. G., Nogueira, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/11209
Resumo: Stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition.
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spelling Biofilms formed on humic substances : response to flow conditions and carbon concentrationsHumic substancesBiofilmFlow velocityCommunity compositionDGGEScience & TechnologyStream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition.Fundação para a Ciência e a Tecnologia (FCT) - bolsa de doutoramento SFRH/BD/18565/2004.Elsevier Ltd.Universidade do MinhoRodrigues, A. L.Pereira, M. A.Janknecht, P.Brito, A. G.Nogueira, R.2010-092010-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11209eng"Bioresource Technology." ISSN 0960-8524. 101:18 (Set. 2010) 6888–6894.0960-852410.1016/j.biortech.2010.03.12120413305http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:41:07Zoai:repositorium.sdum.uminho.pt:1822/11209Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:41:07Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilms formed on humic substances : response to flow conditions and carbon concentrations
title Biofilms formed on humic substances : response to flow conditions and carbon concentrations
spellingShingle Biofilms formed on humic substances : response to flow conditions and carbon concentrations
Rodrigues, A. L.
Humic substances
Biofilm
Flow velocity
Community composition
DGGE
Science & Technology
title_short Biofilms formed on humic substances : response to flow conditions and carbon concentrations
title_full Biofilms formed on humic substances : response to flow conditions and carbon concentrations
title_fullStr Biofilms formed on humic substances : response to flow conditions and carbon concentrations
title_full_unstemmed Biofilms formed on humic substances : response to flow conditions and carbon concentrations
title_sort Biofilms formed on humic substances : response to flow conditions and carbon concentrations
author Rodrigues, A. L.
author_facet Rodrigues, A. L.
Pereira, M. A.
Janknecht, P.
Brito, A. G.
Nogueira, R.
author_role author
author2 Pereira, M. A.
Janknecht, P.
Brito, A. G.
Nogueira, R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, A. L.
Pereira, M. A.
Janknecht, P.
Brito, A. G.
Nogueira, R.
dc.subject.por.fl_str_mv Humic substances
Biofilm
Flow velocity
Community composition
DGGE
Science & Technology
topic Humic substances
Biofilm
Flow velocity
Community composition
DGGE
Science & Technology
description Stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
2010-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/11209
url http://hdl.handle.net/1822/11209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Bioresource Technology." ISSN 0960-8524. 101:18 (Set. 2010) 6888–6894.
0960-8524
10.1016/j.biortech.2010.03.121
20413305
http://www.sciencedirect.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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