Biofilms formed on humic substances : response to flow conditions and carbon concentrations
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/11209 |
Resumo: | Stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition. |
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7160 |
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Biofilms formed on humic substances : response to flow conditions and carbon concentrationsHumic substancesBiofilmFlow velocityCommunity compositionDGGEScience & TechnologyStream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition.Fundação para a Ciência e a Tecnologia (FCT) - bolsa de doutoramento SFRH/BD/18565/2004.Elsevier Ltd.Universidade do MinhoRodrigues, A. L.Pereira, M. A.Janknecht, P.Brito, A. G.Nogueira, R.2010-092010-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11209eng"Bioresource Technology." ISSN 0960-8524. 101:18 (Set. 2010) 6888–6894.0960-852410.1016/j.biortech.2010.03.12120413305http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:41:07Zoai:repositorium.sdum.uminho.pt:1822/11209Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:41:07Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
title |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
spellingShingle |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations Rodrigues, A. L. Humic substances Biofilm Flow velocity Community composition DGGE Science & Technology |
title_short |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
title_full |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
title_fullStr |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
title_full_unstemmed |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
title_sort |
Biofilms formed on humic substances : response to flow conditions and carbon concentrations |
author |
Rodrigues, A. L. |
author_facet |
Rodrigues, A. L. Pereira, M. A. Janknecht, P. Brito, A. G. Nogueira, R. |
author_role |
author |
author2 |
Pereira, M. A. Janknecht, P. Brito, A. G. Nogueira, R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodrigues, A. L. Pereira, M. A. Janknecht, P. Brito, A. G. Nogueira, R. |
dc.subject.por.fl_str_mv |
Humic substances Biofilm Flow velocity Community composition DGGE Science & Technology |
topic |
Humic substances Biofilm Flow velocity Community composition DGGE Science & Technology |
description |
Stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. Thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (HSs) to an increase in flow velocity (0.04–0.10 m s-1) and HSs concentration (9.7 ± 1.0 to 19.8 ± 0.4 mg L-1 C). The highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s-1 without HSs. The bacterial community composition of the biofilm with HSs was characterized by sequences with high similarities (≥97%) to the genus Dokdonella and to the genera Comamonas, Cupriavidus and, Ralstonia. Sequences retrieved from the biofilm without HSs presented high similarities (≥97%) to the genus Sphingomonas and the genus Nitrosospira. Experimental results suggested that the presence of HSs under different concentrations and flow velocities did not significantly enhance the cell density of biofilms but influenced its microbial composition. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09 2010-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/11209 |
url |
http://hdl.handle.net/1822/11209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Bioresource Technology." ISSN 0960-8524. 101:18 (Set. 2010) 6888–6894. 0960-8524 10.1016/j.biortech.2010.03.121 20413305 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Ltd. |
publisher.none.fl_str_mv |
Elsevier Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544704162529280 |