Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation

Detalhes bibliográficos
Autor(a) principal: Silva, M. L. Alves da
Data de Publicação: 2010
Outros Autores: Crawford, Aileen, Mundy, Jenifer, Correlo, V. M., Sol, P., Bhattacharya, Mrinal, Hatton, Paul V., Reis, R. L., Neves, N. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20543
Resumo: Naturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs). The influence of these scaffolds’ porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was studied. The effect of stirred conditions on ECM formation was also assessed. Chitosan-poly(butylene succinate) (CPBS) scaffolds were produced by compression moulding and salt leaching, using a blend of 50% of each material. Different porosities and pore size structures were obtained. BACs were seeded onto CPBS scaffolds using spinner flasks. Constructs were then transferred to the incubator, where half were cultured under stirred conditions, and the other half under static conditions for 4 weeks. Constructs were characterized by scanning electron microscopy, histology procedures, immunolocalization of collagen type I and collagen type II, and dimethylmethylene blue assay for glycosaminoglycan (GAG) quantification. Both materials showed good affinity for cell attachment. Cells colonized the entire scaffolds and were able to produce ECM. Large pores with random geometry improved proteoglycans and collagen type II production. However, that structure has the opposite effect on GAG production. Stirred culture conditions indicate enhancement of GAG production in both types of scaffold.
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spelling Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formationCartilage tissue engineeringChitin/chitosanBovine chondrocyteScaffoldScience & TechnologyNaturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs). The influence of these scaffolds’ porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was studied. The effect of stirred conditions on ECM formation was also assessed. Chitosan-poly(butylene succinate) (CPBS) scaffolds were produced by compression moulding and salt leaching, using a blend of 50% of each material. Different porosities and pore size structures were obtained. BACs were seeded onto CPBS scaffolds using spinner flasks. Constructs were then transferred to the incubator, where half were cultured under stirred conditions, and the other half under static conditions for 4 weeks. Constructs were characterized by scanning electron microscopy, histology procedures, immunolocalization of collagen type I and collagen type II, and dimethylmethylene blue assay for glycosaminoglycan (GAG) quantification. Both materials showed good affinity for cell attachment. Cells colonized the entire scaffolds and were able to produce ECM. Large pores with random geometry improved proteoglycans and collagen type II production. However, that structure has the opposite effect on GAG production. Stirred culture conditions indicate enhancement of GAG production in both types of scaffold.M.L. Alves da Silva would like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for her grant (SFRH/BD/28708/2006), Marie Curie Actions-ALEA JACTA EST (MEST-CT-2004-008104), European NoE EXPERTISSUES (NMP3-CT-2004-500283), IP GENOSTEM (LSHB-CT-2003-503161) and CARTISCAFF (POCTI/SAUIBMA/58982)ElsevierUniversidade do MinhoSilva, M. L. Alves daCrawford, AileenMundy, JeniferCorrelo, V. M.Sol, P.Bhattacharya, MrinalHatton, Paul V.Reis, R. L.Neves, N. M.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20543eng1742-706110.1016/j.actbio.2009.09.00619788942http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:10Zoai:repositorium.sdum.uminho.pt:1822/20543Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:05.692868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
title Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
spellingShingle Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
Silva, M. L. Alves da
Cartilage tissue engineering
Chitin/chitosan
Bovine chondrocyte
Scaffold
Science & Technology
title_short Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
title_full Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
title_fullStr Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
title_full_unstemmed Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
title_sort Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation
author Silva, M. L. Alves da
author_facet Silva, M. L. Alves da
Crawford, Aileen
Mundy, Jenifer
Correlo, V. M.
Sol, P.
Bhattacharya, Mrinal
Hatton, Paul V.
Reis, R. L.
Neves, N. M.
author_role author
author2 Crawford, Aileen
Mundy, Jenifer
Correlo, V. M.
Sol, P.
Bhattacharya, Mrinal
Hatton, Paul V.
Reis, R. L.
Neves, N. M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, M. L. Alves da
Crawford, Aileen
Mundy, Jenifer
Correlo, V. M.
Sol, P.
Bhattacharya, Mrinal
Hatton, Paul V.
Reis, R. L.
Neves, N. M.
dc.subject.por.fl_str_mv Cartilage tissue engineering
Chitin/chitosan
Bovine chondrocyte
Scaffold
Science & Technology
topic Cartilage tissue engineering
Chitin/chitosan
Bovine chondrocyte
Scaffold
Science & Technology
description Naturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs). The influence of these scaffolds’ porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was studied. The effect of stirred conditions on ECM formation was also assessed. Chitosan-poly(butylene succinate) (CPBS) scaffolds were produced by compression moulding and salt leaching, using a blend of 50% of each material. Different porosities and pore size structures were obtained. BACs were seeded onto CPBS scaffolds using spinner flasks. Constructs were then transferred to the incubator, where half were cultured under stirred conditions, and the other half under static conditions for 4 weeks. Constructs were characterized by scanning electron microscopy, histology procedures, immunolocalization of collagen type I and collagen type II, and dimethylmethylene blue assay for glycosaminoglycan (GAG) quantification. Both materials showed good affinity for cell attachment. Cells colonized the entire scaffolds and were able to produce ECM. Large pores with random geometry improved proteoglycans and collagen type II production. However, that structure has the opposite effect on GAG production. Stirred culture conditions indicate enhancement of GAG production in both types of scaffold.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20543
url http://hdl.handle.net/1822/20543
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1742-7061
10.1016/j.actbio.2009.09.006
19788942
http://www.sciencedirect.com/
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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