Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Yan, Leping
Data de Publicação: 2010
Outros Autores: Wang, Yingjun, Rei, Li, Wu, Gang, Caridade, S. G., Fan, Jiabing, Wang, Lingyun, Ji, Peihong, Oliveira, Joaquim M., Oliveira, J. T., Mano, J. F., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/13864
Resumo: This was the first study to use genipin to cross-link collagen and chitosan.
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spelling Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applicationsGenipinCollagenChitosanArticular cartilageTissue engineeringScience & TechnologyThis was the first study to use genipin to cross-link collagen and chitosan.In this study, genipin-cross-linked collagen/chitosan biodegradable porous scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount and genipin concentration on the scaffolds physicochemical properties was evaluated. The morphologies of the scaffolds were characterized by scanning electron microscope (SEM) and cross-linking degree was investigated by ninhydrin assay. Additionally, the mechanical properties of the scaffolds were assessed under dynamic compression. To study the swelling ratio and the biostability of the collagen/chitosan scaffold, in vitro tests were also carried out by immersion of the scaffolds in PBS solution or digestion in collagenase, respectively. The results showed that the morphologies of the scaffolds underwent a fiber-like to a sheet-like structural transition by increasing chitosan amount. Genipin cross-linking remarkably changed the morphologies and pore sizes of the scaffolds when chitosan amount was less than 25%. Either by increasing the chitosan ratio or performing cross-linking treatment, the swelling ratio of the scaffolds can be tailored. The ninhydrin assay demonstrated that the addition of chitosan could obviously increase the cross-linking efficiency. The degradation studies indicated that genipin cross-linking can effectively enhance the biostability of the scaffolds. The biocompatibility of the scaffolds was evaluated by culturing rabbit chondrocytes in vitro. This study demonstrated that a good viability of the chondrocytes seeded on the scaffold was achieved. The SEM analysis has revealed that the chondrocytes adhered well to the surface of the scaffolds and contacted each other. These results suggest that the genipin-cross-linked collagen/chitosan matrix may be a promising formulation for articular cartilage scaffolding.Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period. Grant Number: 2006BA116B04Guangdong Natural Science Foundation. Grant Number: 07300602Natural Science Foundation Team Project of Guangdong. Grant Number: 4205786State Key Program of National Natural Science of China. Grant Number: 50732003National Basic Research Program of China. Grant Number: 2005CB623902WileyUniversidade do MinhoYan, LepingWang, YingjunRei, LiWu, GangCaridade, S. G.Fan, JiabingWang, LingyunJi, PeihongOliveira, Joaquim M.Oliveira, J. T.Mano, J. F.Reis, R. L.2010-07-202010-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/13864eng1549-329610.1002/jbm.a.3286920648541http://onlinelibrary.wiley.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:46Zoai:repositorium.sdum.uminho.pt:1822/13864Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:29.133716Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
title Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
spellingShingle Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
Yan, Leping
Genipin
Collagen
Chitosan
Articular cartilage
Tissue engineering
Science & Technology
title_short Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
title_full Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
title_fullStr Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
title_full_unstemmed Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
title_sort Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications
author Yan, Leping
author_facet Yan, Leping
Wang, Yingjun
Rei, Li
Wu, Gang
Caridade, S. G.
Fan, Jiabing
Wang, Lingyun
Ji, Peihong
Oliveira, Joaquim M.
Oliveira, J. T.
Mano, J. F.
Reis, R. L.
author_role author
author2 Wang, Yingjun
Rei, Li
Wu, Gang
Caridade, S. G.
Fan, Jiabing
Wang, Lingyun
Ji, Peihong
Oliveira, Joaquim M.
Oliveira, J. T.
Mano, J. F.
Reis, R. L.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Yan, Leping
Wang, Yingjun
Rei, Li
Wu, Gang
Caridade, S. G.
Fan, Jiabing
Wang, Lingyun
Ji, Peihong
Oliveira, Joaquim M.
Oliveira, J. T.
Mano, J. F.
Reis, R. L.
dc.subject.por.fl_str_mv Genipin
Collagen
Chitosan
Articular cartilage
Tissue engineering
Science & Technology
topic Genipin
Collagen
Chitosan
Articular cartilage
Tissue engineering
Science & Technology
description This was the first study to use genipin to cross-link collagen and chitosan.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-20
2010-07-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/13864
url https://hdl.handle.net/1822/13864
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1549-3296
10.1002/jbm.a.32869
20648541
http://onlinelibrary.wiley.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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