Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars

Detalhes bibliográficos
Autor(a) principal: Michelin, Michele
Data de Publicação: 2018
Outros Autores: Ruiz, Héctor A., Polizeli, Maria de Lourdes T. M., Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/46776
Resumo: This work presents an integrated and multi-step approach for the recovery and/or application of the lignocellulosic fractions from corncob in the production of high value added compounds as xylo-oligosaccharides, enzymes, fermentable sugars, and lignin in terms of biorefinery concept. For that, liquid hot water followed by enzymatic hydrolysis were used. Liquid hot water was performed using different residence times (1050 minutes) and holding temperature (180200 °C), corresponding to severities (log(R0)) of 3.364.64. The most severe conditions showed higher xylo-oligosaccharides extraction (maximum of 93%) into the hydrolysates and higher recovery of cellulose on pretreated solids (maximum of 65%). Subsequently, hydrolysates and solids were used in the production of xylanases and cellulases, respectively, as well as, pretreated solids were also subjected to enzymatic hydrolysis for the recovery of lignin and fermentable sugars from cellulose. Maximum glucose yield (100%) was achieved for solids pretreated at log(R0) of 4.42 and 5% solid loading.
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spelling Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugarsAutohydrolysisEnzymesSaccharificationAspergillus nigerTrichoderma reeseiScience & TechnologyThis work presents an integrated and multi-step approach for the recovery and/or application of the lignocellulosic fractions from corncob in the production of high value added compounds as xylo-oligosaccharides, enzymes, fermentable sugars, and lignin in terms of biorefinery concept. For that, liquid hot water followed by enzymatic hydrolysis were used. Liquid hot water was performed using different residence times (1050 minutes) and holding temperature (180200 °C), corresponding to severities (log(R0)) of 3.364.64. The most severe conditions showed higher xylo-oligosaccharides extraction (maximum of 93%) into the hydrolysates and higher recovery of cellulose on pretreated solids (maximum of 65%). Subsequently, hydrolysates and solids were used in the production of xylanases and cellulases, respectively, as well as, pretreated solids were also subjected to enzymatic hydrolysis for the recovery of lignin and fermentable sugars from cellulose. Maximum glucose yield (100%) was achieved for solids pretreated at log(R0) of 4.42 and 5% solid loading.Michele Michelin is a recipient of a FCT fellowship (SFRH/BPD/ 100786/2014). ThisstudywassupportedbythePortugueseFoundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-010145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Héctor Ruiz would like to thank the financial support to the Mexican Science and Technology Council (CONACYT, Mexico) for the Basic Science Project-2015-01 (Ref. 254808) and the Energy Sustainability Fund 2014-05 (CONACYT-SENER), Mexican Centre for Innovation in Bioenergy (Cemie-Bio), and Cluster of Bioalcohols (Ref. 249564). We thank Dr. Nelson Lima from MUM (Micoteca da Universidade do Minho, PT) that gently provided the Trichoderma reesei fungi.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoMichelin, MicheleRuiz, Héctor A.Polizeli, Maria de Lourdes T. M.Teixeira, J. A.20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46776engMichelin, Michele; Ruiz, Héctor A.; Polizeli, Maria de Lourdes T. M.; Teixeira, José A., Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars. Bioresource Technology, 247, 582-590, 20180960-852410.1016/j.biortech.2017.09.12828982088http://www.journals.elsevier.com/bioresource-technology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:49Zoai:repositorium.sdum.uminho.pt:1822/46776Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:23.361694Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
title Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
spellingShingle Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
Michelin, Michele
Autohydrolysis
Enzymes
Saccharification
Aspergillus niger
Trichoderma reesei
Science & Technology
title_short Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
title_full Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
title_fullStr Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
title_full_unstemmed Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
title_sort Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars
author Michelin, Michele
author_facet Michelin, Michele
Ruiz, Héctor A.
Polizeli, Maria de Lourdes T. M.
Teixeira, J. A.
author_role author
author2 Ruiz, Héctor A.
Polizeli, Maria de Lourdes T. M.
Teixeira, J. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Michelin, Michele
Ruiz, Héctor A.
Polizeli, Maria de Lourdes T. M.
Teixeira, J. A.
dc.subject.por.fl_str_mv Autohydrolysis
Enzymes
Saccharification
Aspergillus niger
Trichoderma reesei
Science & Technology
topic Autohydrolysis
Enzymes
Saccharification
Aspergillus niger
Trichoderma reesei
Science & Technology
description This work presents an integrated and multi-step approach for the recovery and/or application of the lignocellulosic fractions from corncob in the production of high value added compounds as xylo-oligosaccharides, enzymes, fermentable sugars, and lignin in terms of biorefinery concept. For that, liquid hot water followed by enzymatic hydrolysis were used. Liquid hot water was performed using different residence times (1050 minutes) and holding temperature (180200 °C), corresponding to severities (log(R0)) of 3.364.64. The most severe conditions showed higher xylo-oligosaccharides extraction (maximum of 93%) into the hydrolysates and higher recovery of cellulose on pretreated solids (maximum of 65%). Subsequently, hydrolysates and solids were used in the production of xylanases and cellulases, respectively, as well as, pretreated solids were also subjected to enzymatic hydrolysis for the recovery of lignin and fermentable sugars from cellulose. Maximum glucose yield (100%) was achieved for solids pretreated at log(R0) of 4.42 and 5% solid loading.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/46776
url http://hdl.handle.net/1822/46776
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Michelin, Michele; Ruiz, Héctor A.; Polizeli, Maria de Lourdes T. M.; Teixeira, José A., Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars. Bioresource Technology, 247, 582-590, 2018
0960-8524
10.1016/j.biortech.2017.09.128
28982088
http://www.journals.elsevier.com/bioresource-technology/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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