Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/60723 |
Resumo: | This work targets the valorization of brewers spent grain (BSG) waste by ethanol production, providing strategies for increasing titers in the multiple process steps involved. High solid loadings and use of whole slurry from the pretreatment were evaluated, aiming to achieve high ethanol concentration and yield. As variability in BSG chemical composition presents a challenge for their valorization, six different BSGs were chemically characterized, which allowed the selection of two with high polysaccharide content. High solid loadings (up to 25%) were employed for the pretreatment of selected BSGs by autohydrolysis, an environmentally friendly process, to improve enzymatic saccharification and extract fermentable sugars as oligosaccharides and monosaccharides. As a result, high glucose concentrations (43.7 and 57.7?g L-1) and glucose yield (85.9 and 70.6 %) were obtained from the saccharification of the pretreated BSG whole slurry at 20 and 25% solid loading, respectively. Whole slurries from autohydrolysis were used as substrate for ethanol production by hybrid saccharification and fermentation. Two different Saccharomyces cerevisiae strains were evaluated and high ethanol concentration (42.27?g L-1) at a high yield (94.0 %) was achieved. The results attained by the combined intensification approaches qualify BSG waste as a valuable renewable resource for cost-effective ethanol production. |
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Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadingsBrewers spent grain wastewhole slurryhigh gravity ethanolautohydrolysisindustrial strainsScience & TechnologyThis work targets the valorization of brewers spent grain (BSG) waste by ethanol production, providing strategies for increasing titers in the multiple process steps involved. High solid loadings and use of whole slurry from the pretreatment were evaluated, aiming to achieve high ethanol concentration and yield. As variability in BSG chemical composition presents a challenge for their valorization, six different BSGs were chemically characterized, which allowed the selection of two with high polysaccharide content. High solid loadings (up to 25%) were employed for the pretreatment of selected BSGs by autohydrolysis, an environmentally friendly process, to improve enzymatic saccharification and extract fermentable sugars as oligosaccharides and monosaccharides. As a result, high glucose concentrations (43.7 and 57.7?g L-1) and glucose yield (85.9 and 70.6 %) were obtained from the saccharification of the pretreated BSG whole slurry at 20 and 25% solid loading, respectively. Whole slurries from autohydrolysis were used as substrate for ethanol production by hybrid saccharification and fermentation. Two different Saccharomyces cerevisiae strains were evaluated and high ethanol concentration (42.27?g L-1) at a high yield (94.0 %) was achieved. The results attained by the combined intensification approaches qualify BSG waste as a valuable renewable resource for cost-effective ethanol production.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and COMPETE 2020 (POCI-01-0145-FEDER006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and MultiBiorefinery project (POCI-01-0145-FEDER-016403). Furthermore, authors acknowledge Fermentum-Engenharia das Fermentações Lda., for providing raw material for this work.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPinheiro, T.Coelho, Eduardo João LouroRomani, Aloia PerezDomingues, Lucília2019-11-252019-11-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60723engPinheiro, T.; Coelho, Eduardo; Romaní, Aloia; Domingues, Lucília, Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings. New Biotechnology, 53, 1-8, 20191871-67841876-434710.1016/j.nbt.2019.06.00531195160http://www.journals.elsevier.com/new-biotechnology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:50Zoai:repositorium.sdum.uminho.pt:1822/60723Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:59.143416Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
title |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
spellingShingle |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings Pinheiro, T. Brewers spent grain waste whole slurry high gravity ethanol autohydrolysis industrial strains Science & Technology |
title_short |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
title_full |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
title_fullStr |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
title_full_unstemmed |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
title_sort |
Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings |
author |
Pinheiro, T. |
author_facet |
Pinheiro, T. Coelho, Eduardo João Louro Romani, Aloia Perez Domingues, Lucília |
author_role |
author |
author2 |
Coelho, Eduardo João Louro Romani, Aloia Perez Domingues, Lucília |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pinheiro, T. Coelho, Eduardo João Louro Romani, Aloia Perez Domingues, Lucília |
dc.subject.por.fl_str_mv |
Brewers spent grain waste whole slurry high gravity ethanol autohydrolysis industrial strains Science & Technology |
topic |
Brewers spent grain waste whole slurry high gravity ethanol autohydrolysis industrial strains Science & Technology |
description |
This work targets the valorization of brewers spent grain (BSG) waste by ethanol production, providing strategies for increasing titers in the multiple process steps involved. High solid loadings and use of whole slurry from the pretreatment were evaluated, aiming to achieve high ethanol concentration and yield. As variability in BSG chemical composition presents a challenge for their valorization, six different BSGs were chemically characterized, which allowed the selection of two with high polysaccharide content. High solid loadings (up to 25%) were employed for the pretreatment of selected BSGs by autohydrolysis, an environmentally friendly process, to improve enzymatic saccharification and extract fermentable sugars as oligosaccharides and monosaccharides. As a result, high glucose concentrations (43.7 and 57.7?g L-1) and glucose yield (85.9 and 70.6 %) were obtained from the saccharification of the pretreated BSG whole slurry at 20 and 25% solid loading, respectively. Whole slurries from autohydrolysis were used as substrate for ethanol production by hybrid saccharification and fermentation. Two different Saccharomyces cerevisiae strains were evaluated and high ethanol concentration (42.27?g L-1) at a high yield (94.0 %) was achieved. The results attained by the combined intensification approaches qualify BSG waste as a valuable renewable resource for cost-effective ethanol production. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-25 2019-11-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/60723 |
url |
http://hdl.handle.net/1822/60723 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinheiro, T.; Coelho, Eduardo; Romaní, Aloia; Domingues, Lucília, Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings. New Biotechnology, 53, 1-8, 2019 1871-6784 1876-4347 10.1016/j.nbt.2019.06.005 31195160 http://www.journals.elsevier.com/new-biotechnology/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132580833918976 |