The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11787 |
Resumo: | The determination of left-right body asymmetry in mouse embryos depends on the interplay of molecules In a highly sensitive structure, the node. Here, we show that the localization of Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, Cerl2 protein displays a nodal flow-dependent dynamic behavior that controls the activity of Nodal in the node, and the transmission of the laterality information to the left lateral plate mesoderm (LPM). Our results indicate that Cerl2 initially localizes and prevents the activation of Nodal genetic circuitry on the right side of the embryo, and later its right-to-left translocation shutdowns Nodal activity in the node. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the LPM. Simultaneous genetic removal of both Nodal node inhibitors, Cerl2 and Lefty1, sustains even longer and bilateral his LPM expression. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse nodeLeft-right asymmetryLeft-right axisVertebrate embryoKupffers vesicleLateral plateCerberus-likeFluid-flowHeartHeterotaxiaExpressionThe determination of left-right body asymmetry in mouse embryos depends on the interplay of molecules In a highly sensitive structure, the node. Here, we show that the localization of Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, Cerl2 protein displays a nodal flow-dependent dynamic behavior that controls the activity of Nodal in the node, and the transmission of the laterality information to the left lateral plate mesoderm (LPM). Our results indicate that Cerl2 initially localizes and prevents the activation of Nodal genetic circuitry on the right side of the embryo, and later its right-to-left translocation shutdowns Nodal activity in the node. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the LPM. Simultaneous genetic removal of both Nodal node inhibitors, Cerl2 and Lefty1, sustains even longer and bilateral his LPM expression.F.C.T.; IBB/CBME, LAPublic Library of ScienceSapientiaInacio, Jose ManuelMarques, SaraNakamura, TetsuyaShinohara, KyosukeMeno, ChikaraHamada, HiroshiBelo, Jose Antonio2018-12-07T14:57:58Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11787eng1932-620310.1371/journal.pone.0060406info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:38Zoai:sapientia.ualg.pt:10400.1/11787Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:14.596694Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
title |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
spellingShingle |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node Inacio, Jose Manuel Left-right asymmetry Left-right axis Vertebrate embryo Kupffers vesicle Lateral plate Cerberus-like Fluid-flow Heart Heterotaxia Expression |
title_short |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
title_full |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
title_fullStr |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
title_full_unstemmed |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
title_sort |
The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node |
author |
Inacio, Jose Manuel |
author_facet |
Inacio, Jose Manuel Marques, Sara Nakamura, Tetsuya Shinohara, Kyosuke Meno, Chikara Hamada, Hiroshi Belo, Jose Antonio |
author_role |
author |
author2 |
Marques, Sara Nakamura, Tetsuya Shinohara, Kyosuke Meno, Chikara Hamada, Hiroshi Belo, Jose Antonio |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Inacio, Jose Manuel Marques, Sara Nakamura, Tetsuya Shinohara, Kyosuke Meno, Chikara Hamada, Hiroshi Belo, Jose Antonio |
dc.subject.por.fl_str_mv |
Left-right asymmetry Left-right axis Vertebrate embryo Kupffers vesicle Lateral plate Cerberus-like Fluid-flow Heart Heterotaxia Expression |
topic |
Left-right asymmetry Left-right axis Vertebrate embryo Kupffers vesicle Lateral plate Cerberus-like Fluid-flow Heart Heterotaxia Expression |
description |
The determination of left-right body asymmetry in mouse embryos depends on the interplay of molecules In a highly sensitive structure, the node. Here, we show that the localization of Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, Cerl2 protein displays a nodal flow-dependent dynamic behavior that controls the activity of Nodal in the node, and the transmission of the laterality information to the left lateral plate mesoderm (LPM). Our results indicate that Cerl2 initially localizes and prevents the activation of Nodal genetic circuitry on the right side of the embryo, and later its right-to-left translocation shutdowns Nodal activity in the node. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the LPM. Simultaneous genetic removal of both Nodal node inhibitors, Cerl2 and Lefty1, sustains even longer and bilateral his LPM expression. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2018-12-07T14:57:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11787 |
url |
http://hdl.handle.net/10400.1/11787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 10.1371/journal.pone.0060406 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133267041976320 |