Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA

Detalhes bibliográficos
Autor(a) principal: Nakamura, Tetsuya
Data de Publicação: 2012
Outros Autores: Saito, Daisuke, Kawasumi, Aiko, Shinohara, Kyosuke, Asai, Yasuko, Takaoka, Katsuyoshi, Dong, Fenglan, Takamatsu, Atsuko, A. Belo, José, Mochizuki, Atsushi, Hamada, Hiroshi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/11225
Resumo: Breaking of left-right symmetry in mouse embryos requires fluid flow at the node, but the precise action of the flow has remained unknown. Here we show that the left-right asymmetry of Cerl2 expression around the node, a target of the flow, is determined post-transcriptionally by decay of Cerl2 mRNA in a manner dependent on its 3' untranslated region. Cerl2 mRNA is absent specifically from the apical region of crown cells on the left side of the node. Preferential decay of Cerl2 mRNA on the left is initiated by the leftward flow and further enhanced by the operation of Wnt-Cerl2 interlinked feedback loops, in which Wnt3 upregulates Wnt3 expression and promotes Cerl2 mRNA decay, whereas Cerl2 promotes Wnt degradation. Mathematical modelling and experimental data suggest that these feedback loops behave as a bistable switch that can amplify in a noise-resistant manner a small bias conferred by fluid flow.
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spelling Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNALeft right axisMouse embryoGene expressionAnteroposterior AxisLateral plateBetaSystemNodeCellSegmentationBreaking of left-right symmetry in mouse embryos requires fluid flow at the node, but the precise action of the flow has remained unknown. Here we show that the left-right asymmetry of Cerl2 expression around the node, a target of the flow, is determined post-transcriptionally by decay of Cerl2 mRNA in a manner dependent on its 3' untranslated region. Cerl2 mRNA is absent specifically from the apical region of crown cells on the left side of the node. Preferential decay of Cerl2 mRNA on the left is initiated by the leftward flow and further enhanced by the operation of Wnt-Cerl2 interlinked feedback loops, in which Wnt3 upregulates Wnt3 expression and promotes Cerl2 mRNA decay, whereas Cerl2 promotes Wnt degradation. Mathematical modelling and experimental data suggest that these feedback loops behave as a bistable switch that can amplify in a noise-resistant manner a small bias conferred by fluid flow.Ministry of Education, Culture, Sports, Science, and Technology of Japan; Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation (JST); GCOE of Osaka University; FCTNature Publishing GroupSapientiaNakamura, TetsuyaSaito, DaisukeKawasumi, AikoShinohara, KyosukeAsai, YasukoTakaoka, KatsuyoshiDong, FenglanTakamatsu, AtsukoA. Belo, JoséMochizuki, AtsushiHamada, Hiroshi2018-12-07T14:52:49Z2012-122012-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11225eng2041-172310.1038/ncomms2319info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:22:59Zoai:sapientia.ualg.pt:10400.1/11225Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:02:43.934440Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
title Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
spellingShingle Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
Nakamura, Tetsuya
Left right axis
Mouse embryo
Gene expression
Anteroposterior Axis
Lateral plate
Beta
System
Node
Cell
Segmentation
title_short Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
title_full Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
title_fullStr Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
title_full_unstemmed Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
title_sort Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA
author Nakamura, Tetsuya
author_facet Nakamura, Tetsuya
Saito, Daisuke
Kawasumi, Aiko
Shinohara, Kyosuke
Asai, Yasuko
Takaoka, Katsuyoshi
Dong, Fenglan
Takamatsu, Atsuko
A. Belo, José
Mochizuki, Atsushi
Hamada, Hiroshi
author_role author
author2 Saito, Daisuke
Kawasumi, Aiko
Shinohara, Kyosuke
Asai, Yasuko
Takaoka, Katsuyoshi
Dong, Fenglan
Takamatsu, Atsuko
A. Belo, José
Mochizuki, Atsushi
Hamada, Hiroshi
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Nakamura, Tetsuya
Saito, Daisuke
Kawasumi, Aiko
Shinohara, Kyosuke
Asai, Yasuko
Takaoka, Katsuyoshi
Dong, Fenglan
Takamatsu, Atsuko
A. Belo, José
Mochizuki, Atsushi
Hamada, Hiroshi
dc.subject.por.fl_str_mv Left right axis
Mouse embryo
Gene expression
Anteroposterior Axis
Lateral plate
Beta
System
Node
Cell
Segmentation
topic Left right axis
Mouse embryo
Gene expression
Anteroposterior Axis
Lateral plate
Beta
System
Node
Cell
Segmentation
description Breaking of left-right symmetry in mouse embryos requires fluid flow at the node, but the precise action of the flow has remained unknown. Here we show that the left-right asymmetry of Cerl2 expression around the node, a target of the flow, is determined post-transcriptionally by decay of Cerl2 mRNA in a manner dependent on its 3' untranslated region. Cerl2 mRNA is absent specifically from the apical region of crown cells on the left side of the node. Preferential decay of Cerl2 mRNA on the left is initiated by the leftward flow and further enhanced by the operation of Wnt-Cerl2 interlinked feedback loops, in which Wnt3 upregulates Wnt3 expression and promotes Cerl2 mRNA decay, whereas Cerl2 promotes Wnt degradation. Mathematical modelling and experimental data suggest that these feedback loops behave as a bistable switch that can amplify in a noise-resistant manner a small bias conferred by fluid flow.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
2012-12-01T00:00:00Z
2018-12-07T14:52:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/11225
url http://hdl.handle.net/10400.1/11225
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
10.1038/ncomms2319
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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