Superparamagnetic hybrid microspheres affecting osteoblasts behaviour

Detalhes bibliográficos
Autor(a) principal: Fernandes Patrício, Tatiana Marisa
Data de Publicação: 2018
Outros Autores: Panseri, Silvia, Montesi, Monica, Iafisco, Michele, Sandri, Monica, Tampieri, Anna, Sprio, Simone
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8517
Resumo: The present work describes biomimetic hybrid microspheres made of collagen type I-like peptide matrix (RCP) mineralised with Fe2+/Fe3+ doping hydroxyapatite (RCPFeHA) by a bio-inspired process. Superparamagnetic RCPFeHA microspheres are obtained by emulsification of the hybrid slurries in the presence of citrate ions, to achieve a biomimetic surface functionalisation improving the bioactivity and the dispersion ability in cell culture medium. A biological in vitro study correlates the osteoblast cells behaviour to calcium and iron ions released by the hybrid microspheres in culture media mimicking physiological or inflammatory environment, evidencing a clear triggering of cell activity and bio-resorption ability. In presence of the microspheres, the osteoblast cells maintain their typical morphology and no cell damage were detected, whereas also showing up-regulation of osteogenic markers. The ability of the hybrid microspheres to undergo bio-resorption and release bioactive ions in response to different environmental stimuli without harmful effects opens new perspectives in bone regeneration, as magnetically active bone substitute with potential ability of drug carrier and smart response in the presence of inflammatory states.
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spelling Superparamagnetic hybrid microspheres affecting osteoblasts behaviourBone tissue engineeringCell behaviourIron-substituted apatiteIons releaseBio-inspired mineralisationHybrid microspheresThe present work describes biomimetic hybrid microspheres made of collagen type I-like peptide matrix (RCP) mineralised with Fe2+/Fe3+ doping hydroxyapatite (RCPFeHA) by a bio-inspired process. Superparamagnetic RCPFeHA microspheres are obtained by emulsification of the hybrid slurries in the presence of citrate ions, to achieve a biomimetic surface functionalisation improving the bioactivity and the dispersion ability in cell culture medium. A biological in vitro study correlates the osteoblast cells behaviour to calcium and iron ions released by the hybrid microspheres in culture media mimicking physiological or inflammatory environment, evidencing a clear triggering of cell activity and bio-resorption ability. In presence of the microspheres, the osteoblast cells maintain their typical morphology and no cell damage were detected, whereas also showing up-regulation of osteogenic markers. The ability of the hybrid microspheres to undergo bio-resorption and release bioactive ions in response to different environmental stimuli without harmful effects opens new perspectives in bone regeneration, as magnetically active bone substitute with potential ability of drug carrier and smart response in the presence of inflammatory states.ScienceDirectIC-OnlineFernandes Patrício, Tatiana MarisaPanseri, SilviaMontesi, MonicaIafisco, MicheleSandri, MonicaTampieri, AnnaSprio, Simone2023-05-25T14:25:31Z2018-11-142018-11-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8517engTatiana M. Fernandes Patrício, Silvia Panseri, Monica Montesi, Michele Iafisco, Monica Sandri, Anna Tampieri, Simone Sprio, Superparamagnetic hybrid microspheres affecting osteoblasts behaviour, Materials Science and Engineering: C, Volume 96, 2019, Pages 234-247, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2018.11.0141873-019110.1016/j.msec.2018.11.014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:57:30Zoai:iconline.ipleiria.pt:10400.8/8517Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:13.245167Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
title Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
spellingShingle Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
Fernandes Patrício, Tatiana Marisa
Bone tissue engineering
Cell behaviour
Iron-substituted apatiteIons release
Bio-inspired mineralisation
Hybrid microspheres
title_short Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
title_full Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
title_fullStr Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
title_full_unstemmed Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
title_sort Superparamagnetic hybrid microspheres affecting osteoblasts behaviour
author Fernandes Patrício, Tatiana Marisa
author_facet Fernandes Patrício, Tatiana Marisa
Panseri, Silvia
Montesi, Monica
Iafisco, Michele
Sandri, Monica
Tampieri, Anna
Sprio, Simone
author_role author
author2 Panseri, Silvia
Montesi, Monica
Iafisco, Michele
Sandri, Monica
Tampieri, Anna
Sprio, Simone
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Fernandes Patrício, Tatiana Marisa
Panseri, Silvia
Montesi, Monica
Iafisco, Michele
Sandri, Monica
Tampieri, Anna
Sprio, Simone
dc.subject.por.fl_str_mv Bone tissue engineering
Cell behaviour
Iron-substituted apatiteIons release
Bio-inspired mineralisation
Hybrid microspheres
topic Bone tissue engineering
Cell behaviour
Iron-substituted apatiteIons release
Bio-inspired mineralisation
Hybrid microspheres
description The present work describes biomimetic hybrid microspheres made of collagen type I-like peptide matrix (RCP) mineralised with Fe2+/Fe3+ doping hydroxyapatite (RCPFeHA) by a bio-inspired process. Superparamagnetic RCPFeHA microspheres are obtained by emulsification of the hybrid slurries in the presence of citrate ions, to achieve a biomimetic surface functionalisation improving the bioactivity and the dispersion ability in cell culture medium. A biological in vitro study correlates the osteoblast cells behaviour to calcium and iron ions released by the hybrid microspheres in culture media mimicking physiological or inflammatory environment, evidencing a clear triggering of cell activity and bio-resorption ability. In presence of the microspheres, the osteoblast cells maintain their typical morphology and no cell damage were detected, whereas also showing up-regulation of osteogenic markers. The ability of the hybrid microspheres to undergo bio-resorption and release bioactive ions in response to different environmental stimuli without harmful effects opens new perspectives in bone regeneration, as magnetically active bone substitute with potential ability of drug carrier and smart response in the presence of inflammatory states.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-14
2018-11-14T00:00:00Z
2023-05-25T14:25:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8517
url http://hdl.handle.net/10400.8/8517
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tatiana M. Fernandes Patrício, Silvia Panseri, Monica Montesi, Michele Iafisco, Monica Sandri, Anna Tampieri, Simone Sprio, Superparamagnetic hybrid microspheres affecting osteoblasts behaviour, Materials Science and Engineering: C, Volume 96, 2019, Pages 234-247, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2018.11.014
1873-0191
10.1016/j.msec.2018.11.014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ScienceDirect
publisher.none.fl_str_mv ScienceDirect
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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