Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/665 |
Resumo: | Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosisAnimalsAnimals, Genetically ModifiedBlotting, WesternCell Cycle ProteinsChromatinChromosomal Proteins, Non-HistoneDNADrosophila ProteinsDrosophila melanogasterFemaleImmunoenzyme TechniquesInterphaseMaleMitosisNeuronsPolytene ChromosomesRNA, MessengerReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSalivary GlandsCohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct.FP7 Marie Curie Intra‐European Fellowship; ERC grant.Embo PressARCAEichinger, Christian SKurze, AlexanderOliveira, Raquel ANasmyth, Kim2016-06-27T16:30:28Z2013-01-222013-01-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/665engEichinger, C. S., Kurze, A., Oliveira, R. A., Nasmyth, K. (2013). Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. The EMBO Journal, 32(5), 656–665.10.1038/emboj.2012.346info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:03Zoai:arca.igc.gulbenkian.pt:10400.7/665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:54.137417Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
title |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
spellingShingle |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis Eichinger, Christian S Animals Animals, Genetically Modified Blotting, Western Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA Drosophila Proteins Drosophila melanogaster Female Immunoenzyme Techniques Interphase Male Mitosis Neurons Polytene Chromosomes RNA, Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Salivary Glands |
title_short |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
title_full |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
title_fullStr |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
title_full_unstemmed |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
title_sort |
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis |
author |
Eichinger, Christian S |
author_facet |
Eichinger, Christian S Kurze, Alexander Oliveira, Raquel A Nasmyth, Kim |
author_role |
author |
author2 |
Kurze, Alexander Oliveira, Raquel A Nasmyth, Kim |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Eichinger, Christian S Kurze, Alexander Oliveira, Raquel A Nasmyth, Kim |
dc.subject.por.fl_str_mv |
Animals Animals, Genetically Modified Blotting, Western Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA Drosophila Proteins Drosophila melanogaster Female Immunoenzyme Techniques Interphase Male Mitosis Neurons Polytene Chromosomes RNA, Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Salivary Glands |
topic |
Animals Animals, Genetically Modified Blotting, Western Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA Drosophila Proteins Drosophila melanogaster Female Immunoenzyme Techniques Interphase Male Mitosis Neurons Polytene Chromosomes RNA, Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Salivary Glands |
description |
Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-22 2013-01-22T00:00:00Z 2016-06-27T16:30:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/665 |
url |
http://hdl.handle.net/10400.7/665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eichinger, C. S., Kurze, A., Oliveira, R. A., Nasmyth, K. (2013). Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. The EMBO Journal, 32(5), 656–665. 10.1038/emboj.2012.346 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Embo Press |
publisher.none.fl_str_mv |
Embo Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799130574621769728 |