Revisiting the role of the mother centriole in centriole biogenesis

• Autor(a) principal: Rodrigues-Martins, A
• Data de Publicação: 2007
• Outros Autores: Riparbelli, M, Callaini, G, Glover, D M, Bettencourt-Dias, M
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.7/955
• topic: Animals; Animals, Genetically Modified; Centrioles; Centrosome; Drosophila; Drosophila Proteins; Embryo, Nonmammalian; Embryonic Development; Female; Mitosis; Oocytes

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.