Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24303 |
Resumo: | Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological applications; however, there are few studies on its chemical composition and antimicrobial activity. Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi. The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks. |
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Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarpLentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological applications; however, there are few studies on its chemical composition and antimicrobial activity. Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi. The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.The authors thank Paranaense University, Fundação Araucária, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) -finance code 001-, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support and the fellowship. The authors also thank the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); national funding by FCT, PI, through the institutional scientific employment program-contract for L. Barros’s contract, to the project Valor Natural for the contract of F. Reis (Mobilized Project Norte-01-0247- FEDER-024479), and the Ministry of Education, Science and Technological Development of Republic of Serbia (451-03-68/ 2020-14/200007).Biblioteca Digital do IPBBertéli, Míria B.D.Barros, LillianReis, Filipa S.Ferreira, Isabel C.F.R.Glamočlija, JasminaSoković, MarinaValle, Juliana Silveira doLinde, Giani AndreaRuiz, Suelen PereiraColauto, Nelson Barros2018-01-19T10:00:00Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/24303engBertéli, Míria Benetati Delgado; Barros, Lillian; Reis, Filipa S.; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Sokovič, Marina; Valle, Juliana Silveira Do; Linde, Giani Andrea; Ruiz, Suelen Pereira; Colauto, Nelson Barros (2021). Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp. Food and Function. ISSN 2042-6496. 12:15, p. 6780-67922042-649610.1039/d1fo00656hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:20Zoai:bibliotecadigital.ipb.pt:10198/24303Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:08.886165Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
title |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
spellingShingle |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp Bertéli, Míria B.D. |
title_short |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
title_full |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
title_fullStr |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
title_full_unstemmed |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
title_sort |
Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp |
author |
Bertéli, Míria B.D. |
author_facet |
Bertéli, Míria B.D. Barros, Lillian Reis, Filipa S. Ferreira, Isabel C.F.R. Glamočlija, Jasmina Soković, Marina Valle, Juliana Silveira do Linde, Giani Andrea Ruiz, Suelen Pereira Colauto, Nelson Barros |
author_role |
author |
author2 |
Barros, Lillian Reis, Filipa S. Ferreira, Isabel C.F.R. Glamočlija, Jasmina Soković, Marina Valle, Juliana Silveira do Linde, Giani Andrea Ruiz, Suelen Pereira Colauto, Nelson Barros |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Bertéli, Míria B.D. Barros, Lillian Reis, Filipa S. Ferreira, Isabel C.F.R. Glamočlija, Jasmina Soković, Marina Valle, Juliana Silveira do Linde, Giani Andrea Ruiz, Suelen Pereira Colauto, Nelson Barros |
description |
Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological applications; however, there are few studies on its chemical composition and antimicrobial activity. Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi. The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/24303 |
url |
http://hdl.handle.net/10198/24303 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bertéli, Míria Benetati Delgado; Barros, Lillian; Reis, Filipa S.; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Sokovič, Marina; Valle, Juliana Silveira Do; Linde, Giani Andrea; Ruiz, Suelen Pereira; Colauto, Nelson Barros (2021). Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarp. Food and Function. ISSN 2042-6496. 12:15, p. 6780-6792 2042-6496 10.1039/d1fo00656h |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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