A digital microfluidics platform for loop-mediated isothermal amplification detection

Detalhes bibliográficos
Autor(a) principal: Coelho, Beatriz Jorge
Data de Publicação: 2017
Outros Autores: Veigas, Bruno, Águas, Hugo, Fortunato, Elvira, Martins, Rodrigo, Baptista, Pedro Viana, Igreja, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146292
Resumo: FEDER (Financial Institute for Regional Development) funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). Inn-INDIGO/0002/2015 RADetect for BV.
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spelling A digital microfluidics platform for loop-mediated isothermal amplification detectionc-MycDigital microfluidicsLab-on-a-chipLoop-mediated isothermal amplificationPoint-of-care diagnosticsAnalytical ChemistryAtomic and Molecular Physics, and OpticsBiochemistryInstrumentationElectrical and Electronic EngineeringFEDER (Financial Institute for Regional Development) funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). Inn-INDIGO/0002/2015 RADetect for BV.Digital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities. Here, we demonstrate for the first time the role of coupling DMF and LAMP, in a dedicated device that allows straightforward mixing of LAMP reagents and target DNA, as well as optimum temperature control (reaction droplets undergo a temperature variation of just 0.3°C, for 65°C at the bottom plate). This device is produced using low-temperature and low-cost production processes, adaptable to disposable and flexible substrates. DMF-LAMP is performed with enhanced sensitivity without compromising reaction efficacy or losing reliability and efficiency, by LAMP-amplifying 0.5 ng/µL of target DNA in just 45 min. Moreover, on-chip LAMP was performed in 1.5 µL, a considerably lower volume than standard bench-top reactions.DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNCoelho, Beatriz JorgeVeigas, BrunoÁguas, HugoFortunato, ElviraMartins, RodrigoBaptista, Pedro VianaIgreja, Rui2022-12-16T22:05:33Z2017-11-162017-11-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/146292eng1424-8220PURE: 3801675https://doi.org/10.3390/s17112616info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:22Zoai:run.unl.pt:10362/146292Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:34.502671Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A digital microfluidics platform for loop-mediated isothermal amplification detection
title A digital microfluidics platform for loop-mediated isothermal amplification detection
spellingShingle A digital microfluidics platform for loop-mediated isothermal amplification detection
Coelho, Beatriz Jorge
c-Myc
Digital microfluidics
Lab-on-a-chip
Loop-mediated isothermal amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
title_short A digital microfluidics platform for loop-mediated isothermal amplification detection
title_full A digital microfluidics platform for loop-mediated isothermal amplification detection
title_fullStr A digital microfluidics platform for loop-mediated isothermal amplification detection
title_full_unstemmed A digital microfluidics platform for loop-mediated isothermal amplification detection
title_sort A digital microfluidics platform for loop-mediated isothermal amplification detection
author Coelho, Beatriz Jorge
author_facet Coelho, Beatriz Jorge
Veigas, Bruno
Águas, Hugo
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro Viana
Igreja, Rui
author_role author
author2 Veigas, Bruno
Águas, Hugo
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro Viana
Igreja, Rui
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCM - Departamento de Ciência dos Materiais
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Coelho, Beatriz Jorge
Veigas, Bruno
Águas, Hugo
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro Viana
Igreja, Rui
dc.subject.por.fl_str_mv c-Myc
Digital microfluidics
Lab-on-a-chip
Loop-mediated isothermal amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
topic c-Myc
Digital microfluidics
Lab-on-a-chip
Loop-mediated isothermal amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
description FEDER (Financial Institute for Regional Development) funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). Inn-INDIGO/0002/2015 RADetect for BV.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-16
2017-11-16T00:00:00Z
2022-12-16T22:05:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146292
url http://hdl.handle.net/10362/146292
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8220
PURE: 3801675
https://doi.org/10.3390/s17112616
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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