Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers

Detalhes bibliográficos
Autor(a) principal: Oliveira, Beatriz
Data de Publicação: 2020
Outros Autores: Veigas, Bruno, Fernandes, Alexandra R., Águas, Hugo, Martins, Rodrigo, Fortunato, Elvira, Baptista, Pedro Viana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/115143
Resumo: UID/CTM/50025/2019 UID/Multi/04378/2019 Inn-INDIGO/0002/2015 PTDC/BTM-SAL/31201/2017 SFRH/BPD/124311/2016
id RCAP_dc58242d007ef76c0174d2fd06460c7b
oai_identifier_str oai:run.unl.pt:10362/115143
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkersDigital amplificationIntegrated microfluidics deviceLab-on-a-chipLoop-mediated isothermal amplificationOncogenesAnalytical ChemistryBiochemistryAtomic and Molecular Physics, and OpticsInstrumentationElectrical and Electronic EngineeringSDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureUID/CTM/50025/2019 UID/Multi/04378/2019 Inn-INDIGO/0002/2015 PTDC/BTM-SAL/31201/2017 SFRH/BPD/124311/2016Microfluidic (MF) advancements have been leveraged toward the development of state-of-the-art platforms for molecular diagnostics, where isothermal amplification schemes allow for further simplification of DNA detection and quantification protocols. The MF integration with loop-mediated isothermal amplification (LAMP) is today the focus of a new generation of chip-based devices for molecular detection, aiming at fast and automated nucleic acid analysis. Here, we combined MF with droplet digital LAMP (ddLAMP) on an all-in-one device that allows for droplet generation, target amplification, and absolute quantification. This multilayer 3D chip was developed in less than 30 minutes by using a low-cost and extremely adaptable production process that exploits direct laser writing technology in “Shrinky-dinks” polystyrene sheets. ddLAMP and target quantification were performed directly on-chip, showing a high correlation between target concentration and positive droplet score. We validated this integrated chip via the amplification of targets ranging from five to 500,000 copies/reaction. Furthermore, on-chip amplification was performed in a 10 µL volume, attaining a limit of detection of five copies/µL under 60 min. This technology was applied to quantify a cancer biomarker, c-MYC, but it can be further extended to any other disease biomarker.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNOliveira, BeatrizVeigas, BrunoFernandes, Alexandra R.Águas, HugoMartins, RodrigoFortunato, ElviraBaptista, Pedro Viana2021-04-07T22:15:13Z2020-03-142020-03-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/115143eng1424-8220PURE: 18832737https://doi.org/10.3390/s20061624info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:57:47Zoai:run.unl.pt:10362/115143Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:41.785877Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
title Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
spellingShingle Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
Oliveira, Beatriz
Digital amplification
Integrated microfluidics device
Lab-on-a-chip
Loop-mediated isothermal amplification
Oncogenes
Analytical Chemistry
Biochemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
title_short Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
title_full Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
title_fullStr Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
title_full_unstemmed Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
title_sort Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers
author Oliveira, Beatriz
author_facet Oliveira, Beatriz
Veigas, Bruno
Fernandes, Alexandra R.
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
Baptista, Pedro Viana
author_role author
author2 Veigas, Bruno
Fernandes, Alexandra R.
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
Baptista, Pedro Viana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
RUN
dc.contributor.author.fl_str_mv Oliveira, Beatriz
Veigas, Bruno
Fernandes, Alexandra R.
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
Baptista, Pedro Viana
dc.subject.por.fl_str_mv Digital amplification
Integrated microfluidics device
Lab-on-a-chip
Loop-mediated isothermal amplification
Oncogenes
Analytical Chemistry
Biochemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
topic Digital amplification
Integrated microfluidics device
Lab-on-a-chip
Loop-mediated isothermal amplification
Oncogenes
Analytical Chemistry
Biochemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
description UID/CTM/50025/2019 UID/Multi/04378/2019 Inn-INDIGO/0002/2015 PTDC/BTM-SAL/31201/2017 SFRH/BPD/124311/2016
publishDate 2020
dc.date.none.fl_str_mv 2020-03-14
2020-03-14T00:00:00Z
2021-04-07T22:15:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/115143
url http://hdl.handle.net/10362/115143
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8220
PURE: 18832737
https://doi.org/10.3390/s20061624
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138038432923648

Registros relacionados