Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models

Detalhes bibliográficos
Autor(a) principal: Fraga, Ana Catarina Freire
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/59225
Resumo: Tese de mestrado, Biologia Molecular e Genética , 2023, Universidade de Lisboa, Faculdade de Ciências
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spelling Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine modelsMaláriaCOVID-19coinfeçãoPlasmodiumSARS-CoV-2Teses de mestrado - 2023Departamento de Biologia VegetalTese de mestrado, Biologia Molecular e Genética , 2023, Universidade de Lisboa, Faculdade de CiênciasCoronavirus disease 2019 (COVID-19) and malaria, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Plasmodium parasites, respectively, share geographical distribution, in regions where the latter disease is endemic. Because of this geographic overlap, coinfection cases have emerged, albeit with variable clinical presentations, ranging from faster clinical recovery to worse health outcomes. Given the current epidemiologic status of both diseases, the occurrence of co-infections between SARS-CoV-2 and Plasmodium is likely to persist. Thus far, epidemiologic studies and case reports have yielded insufficient data on the reciprocal impact of the two pathogens on either infection and related diseases. As such, an experimental approach of this coinfection is critical for the understanding of how these pathogens interact with each other and the impact of this interaction on the progression of either disease. We established a unique and innovative coinfection model to address this issue experimentally for the first time, employing either transgenic mice expressing SARS-CoV-2’s human receptor for cell invasion – the human angiotensin-converting enzyme 2 (hACE2) – or wild-type mice in combination with human- and mouse-infective variants of SARS-CoV-2, respectively, and the rodent malaria parasite, P. berghei. Our results demonstrate for the first time that an ongoing SARS-CoV-2 infection impacts the outcomes of a subsequent Plasmodium infection. Our data shows that a primary infection by a viral variant that causes a severe disease phenotype leads to an exacerbated anti-viral immune response that markedly impairs a subsequent liver infection by the malaria parasite. Additionally, we demonstrate that a primary infection by a viral variant that causes an attenuated disease phenotype reduces malaria severity in mice subsequently infected with P. berghei, partially protecting these animals from experimental cerebral malaria and increasing their survival. Our results have unveiled a hitherto unknown virus-parasite interaction that could have important epidemiological and clinical repercussions in malaria-endemic regions, particularly regarding the management and control of the diseases caused by both pathogens. This work paves the way for the development of other models of co-infection between Plasmodium and respiratory viruses in relevant in vivo models of disease and we anticipate that it will serve as a steppingstone for further research on coinfections, thus filling an important knowledge gap regarding complex disease presentations.Prudêncio, Jorge Miguel MartinsZilhão, Rita, 1959-Repositório da Universidade de LisboaFraga, Ana Catarina Freire2024-01-22T01:32:40Z202320222023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/59225enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-29T01:19:26Zoai:repositorio.ul.pt:10451/59225Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:09:11.206054Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
title Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
spellingShingle Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
Fraga, Ana Catarina Freire
Malária
COVID-19
coinfeção
Plasmodium
SARS-CoV-2
Teses de mestrado - 2023
Departamento de Biologia Vegetal
title_short Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
title_full Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
title_fullStr Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
title_full_unstemmed Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
title_sort Plasmodium berghei/SARS-CoV-2 co-infection phenotype in murine models
author Fraga, Ana Catarina Freire
author_facet Fraga, Ana Catarina Freire
author_role author
dc.contributor.none.fl_str_mv Prudêncio, Jorge Miguel Martins
Zilhão, Rita, 1959-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fraga, Ana Catarina Freire
dc.subject.por.fl_str_mv Malária
COVID-19
coinfeção
Plasmodium
SARS-CoV-2
Teses de mestrado - 2023
Departamento de Biologia Vegetal
topic Malária
COVID-19
coinfeção
Plasmodium
SARS-CoV-2
Teses de mestrado - 2023
Departamento de Biologia Vegetal
description Tese de mestrado, Biologia Molecular e Genética , 2023, Universidade de Lisboa, Faculdade de Ciências
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023-01-01T00:00:00Z
2024-01-22T01:32:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/59225
url http://hdl.handle.net/10451/59225
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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