Bioanalytical approach-ageing, exercise and oxidative stress

• Autor(a) principal: Valado, Ana
• Data de Publicação: 2020
• Outros Autores: Lopes, Diana, Osório, Nádia, Caseiro, Armando, Figueiredo, João, Patrício, Cristina, Pacheco, Maria Paula, Oostingh, Gertie Janneke, Gabriel, António José dos Santos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.26/35835
• topic: Ageing; Exercise; Nitric oxide; Uric acid; Ageing process; Signalling pathway; Postponement; Biological process; Envelhecimento; Exercício físico; Óxido nítrico; Ácido úrico; Processos biológicos

Abstract: Exercise interferes with the ageing, causing changes in markers of the antioxidant system, such as nitric oxide (NO) and uric acid. Aim: The objective of this study was to evaluate if regular exercise affects the ageing process by causing changes in the antioxidant markers, nitric oxide and uric acid, in individuals aged ≥ 65 years, subjected to an exercise plan. Methods: The study involved 12 participants distributed in an experimental and control group. The experimental group performed exercises guided by a physiotherapist during 12 weeks. Three blood samples were collected from participants: T0, at the start of the study; T1, after 12 weeks of exercise and T2, 12 weeks after finishing the exercise. NO and uric acid were quantified with commercial kits. The statistics were performed using SPSS and the ANOVA and Student t-tests were applied. Result: In the experimental group, the mean values of NO showed an increase between T0 and T1, with a slight decrease between T1 and T2. Similar effects were observed for the uric acid concentration in the experimental group, with statistical significance from T0 to T1. Conclusion: After exercise, there was an increase in blood uric acid and NO levels, highlighting the importance of moderate and controlled exercise practice. Therefore, exercise seems relevant and beneficial in activating antioxidant mechanisms.