Exploring oxidative stress pathways in Geobacter sulfurreducens

Detalhes bibliográficos
Autor(a) principal: Portela, Pilar C.
Data de Publicação: 2023
Outros Autores: Morgado, Leonor, Silva, Marta A., Denkhaus, Lukas, Einsle, Oliver, Salgueiro, Carlos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/164359
Resumo: Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Publisher Copyright: Copyright © 2023 Portela, Morgado, Silva, Denkhaus, Einsle and Salgueiro.
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spelling Exploring oxidative stress pathways in Geobacter sulfurreducensthe redox network between MacA peroxidase and triheme periplasmic cytochromescytochromeelectron transferGeobacterNMRoxidative stressperoxidaseprotein–protein interactionsMicrobiologyMicrobiology (medical)Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Publisher Copyright: Copyright © 2023 Portela, Morgado, Silva, Denkhaus, Einsle and Salgueiro.The recent reclassification of the strict anaerobe Geobacter sulfurreducens bacterium as aerotolerant brought attention for oxidative stress protection pathways. Although the electron transfer pathways for oxygen detoxification are not well established, evidence was obtained for the formation of a redox complex between the periplasmic triheme cytochrome PpcA and the diheme cytochrome peroxidase MacA. In the latter, the reduction of the high-potential heme triggers a conformational change that displaces the axial histidine of the low-potential heme with peroxidase activity. More recently, a possible involvement of the triheme periplasmic cytochrome family (PpcA-E) in the protection from oxidative stress in G. sulfurreducens was suggested. To evaluate this hypothesis, we investigated the electron transfer reaction and the biomolecular interaction between each PpcA-E cytochrome and MacA. Using a newly developed method that relies on the different NMR spectral signatures of the heme proteins, we directly monitored the electron transfer reaction from reduced PpcA-E cytochromes to oxidized MacA. The results obtained showed a complete electron transfer from the cytochromes to the high-potential heme of MacA. This highlights PpcA-E cytochromes’ efficient role in providing the necessary reducing power to mitigate oxidative stress situations, hence contributing to a better knowledge of oxidative stress protection pathways in G. sulfurreducens.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNPortela, Pilar C.Morgado, LeonorSilva, Marta A.Denkhaus, LukasEinsle, OliverSalgueiro, Carlos A.2024-03-02T00:26:29Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/164359eng1664-302XPURE: 84397255https://doi.org/10.3389/fmicb.2023.1253114info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:52:05Zoai:run.unl.pt:10362/164359Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:09.477299Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploring oxidative stress pathways in Geobacter sulfurreducens
the redox network between MacA peroxidase and triheme periplasmic cytochromes
title Exploring oxidative stress pathways in Geobacter sulfurreducens
spellingShingle Exploring oxidative stress pathways in Geobacter sulfurreducens
Portela, Pilar C.
cytochrome
electron transfer
Geobacter
NMR
oxidative stress
peroxidase
protein–protein interactions
Microbiology
Microbiology (medical)
title_short Exploring oxidative stress pathways in Geobacter sulfurreducens
title_full Exploring oxidative stress pathways in Geobacter sulfurreducens
title_fullStr Exploring oxidative stress pathways in Geobacter sulfurreducens
title_full_unstemmed Exploring oxidative stress pathways in Geobacter sulfurreducens
title_sort Exploring oxidative stress pathways in Geobacter sulfurreducens
author Portela, Pilar C.
author_facet Portela, Pilar C.
Morgado, Leonor
Silva, Marta A.
Denkhaus, Lukas
Einsle, Oliver
Salgueiro, Carlos A.
author_role author
author2 Morgado, Leonor
Silva, Marta A.
Denkhaus, Lukas
Einsle, Oliver
Salgueiro, Carlos A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Portela, Pilar C.
Morgado, Leonor
Silva, Marta A.
Denkhaus, Lukas
Einsle, Oliver
Salgueiro, Carlos A.
dc.subject.por.fl_str_mv cytochrome
electron transfer
Geobacter
NMR
oxidative stress
peroxidase
protein–protein interactions
Microbiology
Microbiology (medical)
topic cytochrome
electron transfer
Geobacter
NMR
oxidative stress
peroxidase
protein–protein interactions
Microbiology
Microbiology (medical)
description Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT – Portugal) through the following grants: PTDC/BIA-BQM/4967/2020 (to CS), 2020.04717.BD (to PP), EXPL/BIA-BQM/0770/2021 (to LM), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (Associate Laboratory i4HB), and Deutsche Forschungsgemeinschaft (CRC 1381, project ID 403222702; to OE). The NMR spectrometers are part of the National NMR Network (PTNMR) and are supported by FCT-MCTES (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC. Publisher Copyright: Copyright © 2023 Portela, Morgado, Silva, Denkhaus, Einsle and Salgueiro.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2023-10-01T00:00:00Z
2024-03-02T00:26:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/164359
url http://hdl.handle.net/10362/164359
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 84397255
https://doi.org/10.3389/fmicb.2023.1253114
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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