Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/24290 https://doi.org/10.3390/v10080416 |
Resumo: | RNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N. benthamiana 16C plants. CP and p6 showed suppressor activity, with CP showing significantly higher activity than p6, yet activity that was lower than the full OMMV, suggesting a complementary action of CP and p6. These viral suppressors were then used to induce OMMV resistance in plants based on RNA silencing. Two hairpin constructs targeting each suppressor were agroinfiltrated in N. benthamiana plants, which were then inoculated with OMMV RNA. When silencing of both suppressors was achieved, a significant reduction in viral accumulation and symptom attenuation was observed as compared to those of the controls, as well as to when each construct was used alone, proving them to be effective against OMMV infection. This is the first time that a silencing suppressor was found in a necrovirus, and that two independent proteins act as silencing suppressors in a virus member of the Tombusviridae family. |
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Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMVRNA silencingPlant ProtectionResistancePlant VirusesSuppressorsRNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N. benthamiana 16C plants. CP and p6 showed suppressor activity, with CP showing significantly higher activity than p6, yet activity that was lower than the full OMMV, suggesting a complementary action of CP and p6. These viral suppressors were then used to induce OMMV resistance in plants based on RNA silencing. Two hairpin constructs targeting each suppressor were agroinfiltrated in N. benthamiana plants, which were then inoculated with OMMV RNA. When silencing of both suppressors was achieved, a significant reduction in viral accumulation and symptom attenuation was observed as compared to those of the controls, as well as to when each construct was used alone, proving them to be effective against OMMV infection. This is the first time that a silencing suppressor was found in a necrovirus, and that two independent proteins act as silencing suppressors in a virus member of the Tombusviridae family.Viruses - MDPI2019-01-28T17:43:57Z2019-01-282018-08-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/24290http://hdl.handle.net/10174/24290https://doi.org/10.3390/v10080416porVaranda et al., 2018https://www.ncbi.nlm.nih.gov/pubmed/30096871ICAAMcarlavaranda@uevora.ptpmateratski@uevora.ptmdcc@uevora.pticlara@uevora.ptgnolasco@ualg.ptmrff@uevora.pt581Varanda, CarlaMateratski, PatrickCampos, Maria DoroteiaClara, Maria IvoneNolasco, GustavoFélix, Maria do Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:17:01Zoai:dspace.uevora.pt:10174/24290Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:53.739406Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
title |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
spellingShingle |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV Varanda, Carla RNA silencing Plant Protection Resistance Plant Viruses Suppressors |
title_short |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
title_full |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
title_fullStr |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
title_full_unstemmed |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
title_sort |
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV |
author |
Varanda, Carla |
author_facet |
Varanda, Carla Materatski, Patrick Campos, Maria Doroteia Clara, Maria Ivone Nolasco, Gustavo Félix, Maria do Rosário |
author_role |
author |
author2 |
Materatski, Patrick Campos, Maria Doroteia Clara, Maria Ivone Nolasco, Gustavo Félix, Maria do Rosário |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Varanda, Carla Materatski, Patrick Campos, Maria Doroteia Clara, Maria Ivone Nolasco, Gustavo Félix, Maria do Rosário |
dc.subject.por.fl_str_mv |
RNA silencing Plant Protection Resistance Plant Viruses Suppressors |
topic |
RNA silencing Plant Protection Resistance Plant Viruses Suppressors |
description |
RNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N. benthamiana 16C plants. CP and p6 showed suppressor activity, with CP showing significantly higher activity than p6, yet activity that was lower than the full OMMV, suggesting a complementary action of CP and p6. These viral suppressors were then used to induce OMMV resistance in plants based on RNA silencing. Two hairpin constructs targeting each suppressor were agroinfiltrated in N. benthamiana plants, which were then inoculated with OMMV RNA. When silencing of both suppressors was achieved, a significant reduction in viral accumulation and symptom attenuation was observed as compared to those of the controls, as well as to when each construct was used alone, proving them to be effective against OMMV infection. This is the first time that a silencing suppressor was found in a necrovirus, and that two independent proteins act as silencing suppressors in a virus member of the Tombusviridae family. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-09T00:00:00Z 2019-01-28T17:43:57Z 2019-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/24290 http://hdl.handle.net/10174/24290 https://doi.org/10.3390/v10080416 |
url |
http://hdl.handle.net/10174/24290 https://doi.org/10.3390/v10080416 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Varanda et al., 2018 https://www.ncbi.nlm.nih.gov/pubmed/30096871 ICAAM carlavaranda@uevora.pt pmateratski@uevora.pt mdcc@uevora.pt iclara@uevora.pt gnolasco@ualg.pt mrff@uevora.pt 581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Viruses - MDPI |
publisher.none.fl_str_mv |
Viruses - MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136630945087488 |