Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

Detalhes bibliográficos
Autor(a) principal: Silva, Germana Michelle de Medeiros e
Data de Publicação: 2013
Outros Autores: Marques, Daniela Araújo Viana, Porto, Tatiana Souza, Lima Filho, José L., Teixeira, J. A., Pessoa Júnior, Adalberto, Porto, Ana L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/24927
Resumo: Extraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG) – phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 24 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)-rich phase with the highest partition coefficient (K = 37.00) obtained in the system 20% (w/w) PEG 1500 (g/mol)-14% (w/w) phosphate at pH 8.0. The system that allowed for the highest extraction consisted of 15% (w/w) PEG 3350-12% (w/w) phosphate, at pH 7.0. In these conditions, a purification factor of 1.51 was obtained for a partition coefficient of 6.41 with the fibrinolytic activity (FA) retained in the top phase. Obtained results confirm the interest of considering ATPS as an alternative extraction method for fibrinolytic protease from Streptomyces sp. DPUA1576.
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spelling Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systemsAqueous two phase systemFibrinolytic proteasePartitionPEG/phosphateExtractionScience & TechnologyExtraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG) – phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 24 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)-rich phase with the highest partition coefficient (K = 37.00) obtained in the system 20% (w/w) PEG 1500 (g/mol)-14% (w/w) phosphate at pH 8.0. The system that allowed for the highest extraction consisted of 15% (w/w) PEG 3350-12% (w/w) phosphate, at pH 7.0. In these conditions, a purification factor of 1.51 was obtained for a partition coefficient of 6.41 with the fibrinolytic activity (FA) retained in the top phase. Obtained results confirm the interest of considering ATPS as an alternative extraction method for fibrinolytic protease from Streptomyces sp. DPUA1576.ElsevierElsevier BVUniversidade do MinhoSilva, Germana Michelle de Medeiros eMarques, Daniela Araújo VianaPorto, Tatiana SouzaLima Filho, José L.Teixeira, J. A.Pessoa Júnior, AdalbertoPorto, Ana L. F.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24927eng0378-38120378-381210.1016/j.fluid.2012.11.033info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:22Zoai:repositorium.sdum.uminho.pt:1822/24927Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:02.808711Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
title Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
spellingShingle Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
Silva, Germana Michelle de Medeiros e
Aqueous two phase system
Fibrinolytic protease
Partition
PEG/phosphate
Extraction
Science & Technology
title_short Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
title_full Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
title_fullStr Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
title_full_unstemmed Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
title_sort Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems
author Silva, Germana Michelle de Medeiros e
author_facet Silva, Germana Michelle de Medeiros e
Marques, Daniela Araújo Viana
Porto, Tatiana Souza
Lima Filho, José L.
Teixeira, J. A.
Pessoa Júnior, Adalberto
Porto, Ana L. F.
author_role author
author2 Marques, Daniela Araújo Viana
Porto, Tatiana Souza
Lima Filho, José L.
Teixeira, J. A.
Pessoa Júnior, Adalberto
Porto, Ana L. F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Germana Michelle de Medeiros e
Marques, Daniela Araújo Viana
Porto, Tatiana Souza
Lima Filho, José L.
Teixeira, J. A.
Pessoa Júnior, Adalberto
Porto, Ana L. F.
dc.subject.por.fl_str_mv Aqueous two phase system
Fibrinolytic protease
Partition
PEG/phosphate
Extraction
Science & Technology
topic Aqueous two phase system
Fibrinolytic protease
Partition
PEG/phosphate
Extraction
Science & Technology
description Extraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG) – phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 24 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)-rich phase with the highest partition coefficient (K = 37.00) obtained in the system 20% (w/w) PEG 1500 (g/mol)-14% (w/w) phosphate at pH 8.0. The system that allowed for the highest extraction consisted of 15% (w/w) PEG 3350-12% (w/w) phosphate, at pH 7.0. In these conditions, a purification factor of 1.51 was obtained for a partition coefficient of 6.41 with the fibrinolytic activity (FA) retained in the top phase. Obtained results confirm the interest of considering ATPS as an alternative extraction method for fibrinolytic protease from Streptomyces sp. DPUA1576.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/24927
url http://hdl.handle.net/1822/24927
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0378-3812
0378-3812
10.1016/j.fluid.2012.11.033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier BV
publisher.none.fl_str_mv Elsevier
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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