Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System

Detalhes bibliográficos
Autor(a) principal: Kirsch,Larissa de Souza
Data de Publicação: 2012
Outros Autores: Pinto,Ana Carolina dos Santos, Teixeira,Maria Francisca Simas, Porto,Tatiana Souza, Porto,Ana Lúcia Figueiredo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000004
Resumo: A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.
id SBQ-3_d4d338a01559aac707d4a19f5beaccb0
oai_identifier_str oai:scielo:S0100-40422012001000004
network_acronym_str SBQ-3
network_name_str Química Nova (Online)
repository_id_str
spelling Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase SystemLentinus citrinusproteasesaqueous two-phase systemsA full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000004Química Nova v.35 n.10 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012001000004info:eu-repo/semantics/openAccessKirsch,Larissa de SouzaPinto,Ana Carolina dos SantosTeixeira,Maria Francisca SimasPorto,Tatiana SouzaPorto,Ana Lúcia Figueiredoeng2021-02-11T00:00:00Zoai:scielo:S0100-40422012001000004Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2021-02-11T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
title Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
spellingShingle Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
Kirsch,Larissa de Souza
Lentinus citrinus
proteases
aqueous two-phase systems
title_short Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
title_full Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
title_fullStr Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
title_full_unstemmed Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
title_sort Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
author Kirsch,Larissa de Souza
author_facet Kirsch,Larissa de Souza
Pinto,Ana Carolina dos Santos
Teixeira,Maria Francisca Simas
Porto,Tatiana Souza
Porto,Ana Lúcia Figueiredo
author_role author
author2 Pinto,Ana Carolina dos Santos
Teixeira,Maria Francisca Simas
Porto,Tatiana Souza
Porto,Ana Lúcia Figueiredo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Kirsch,Larissa de Souza
Pinto,Ana Carolina dos Santos
Teixeira,Maria Francisca Simas
Porto,Tatiana Souza
Porto,Ana Lúcia Figueiredo
dc.subject.por.fl_str_mv Lentinus citrinus
proteases
aqueous two-phase systems
topic Lentinus citrinus
proteases
aqueous two-phase systems
description A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422012001000004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.35 n.10 2012
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318113969143808

Registros relacionados