The influence of the mode of administration in the dissemination of three coliphages in chickens

Detalhes bibliográficos
Autor(a) principal: Oliveira, A.
Data de Publicação: 2009
Outros Autores: Sereno, R., Nicolau, Ana, Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9470
Resumo: Escherichia coli can cause severe respiratory and systemic infections in chickens, and it is often associated with significant economic losses in the poultry industry. Bacteriophages (phages) have been shown to be potential alternatives to the antibiotics in the treatment of bacterial infections. To accomplish that, phage particles must be able to reach and remain active in the infected organs. The present work aims at evaluating the effect of the route of administration and the dosage in the dissemination of 3 coliphages in the chicken’s organs. In vivo trials were conducted by infecting chickens orally, spray, and i.m. with 106, 107, and 108 plaque-forming units/mL suspensions of 3 lytic phages: phi F78E (Myoviridae), phi F258E (Siphoviridae), and phi F61E (Myoviridae). Birds were killed 3, 10, and 24 h after challenge and the phage titer was measured in lungs and air sacs membranes, liver, duodenum, and spleen. When administered by spray, the 3 phages reached the respiratory tract within 3 h. Oral administration also allowed all phages to be recovered in lungs, but only phi F78E was recovered from the duodenum, the liver, and the spleen. These differences can be explained by the possible replication of phi F78E in commensal E. coli strains present in the chicken gut, thus leading to a higher concentration of this phage in the intestines that resulted in systemic circulation of phage with consequent phage in organs. When phages were administered i.m., they were found in all of the collected organs. Despite this better response, i.m. administration is a nonpracticable way of protecting a large number of birds in a poultry unit. In general, the results suggest that oral administration and spray allowed phages to reach and to remain active in the respiratory tract and can, therefore, be considered promising administration routes to treat respiratory E. coli infections in the poultry industry.
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spelling The influence of the mode of administration in the dissemination of three coliphages in chickensBacteriophageEscherichia coli respiratory infectionDisseminationChickenScience & TechnologyEscherichia coli can cause severe respiratory and systemic infections in chickens, and it is often associated with significant economic losses in the poultry industry. Bacteriophages (phages) have been shown to be potential alternatives to the antibiotics in the treatment of bacterial infections. To accomplish that, phage particles must be able to reach and remain active in the infected organs. The present work aims at evaluating the effect of the route of administration and the dosage in the dissemination of 3 coliphages in the chicken’s organs. In vivo trials were conducted by infecting chickens orally, spray, and i.m. with 106, 107, and 108 plaque-forming units/mL suspensions of 3 lytic phages: phi F78E (Myoviridae), phi F258E (Siphoviridae), and phi F61E (Myoviridae). Birds were killed 3, 10, and 24 h after challenge and the phage titer was measured in lungs and air sacs membranes, liver, duodenum, and spleen. When administered by spray, the 3 phages reached the respiratory tract within 3 h. Oral administration also allowed all phages to be recovered in lungs, but only phi F78E was recovered from the duodenum, the liver, and the spleen. These differences can be explained by the possible replication of phi F78E in commensal E. coli strains present in the chicken gut, thus leading to a higher concentration of this phage in the intestines that resulted in systemic circulation of phage with consequent phage in organs. When phages were administered i.m., they were found in all of the collected organs. Despite this better response, i.m. administration is a nonpracticable way of protecting a large number of birds in a poultry unit. In general, the results suggest that oral administration and spray allowed phages to reach and to remain active in the respiratory tract and can, therefore, be considered promising administration routes to treat respiratory E. coli infections in the poultry industry.Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BDE/15508/2004Poultry Science AssociationUniversidade do MinhoOliveira, A.Sereno, R.Nicolau, AnaAzeredo, Joana2009-042009-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9470eng"Poultry Science". ISSN 0032-5791. 88:4 (2009) 728-733.0032-579110.3382/ps.2008-0037819276415info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:36Zoai:repositorium.sdum.uminho.pt:1822/9470Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:45.361940Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The influence of the mode of administration in the dissemination of three coliphages in chickens
title The influence of the mode of administration in the dissemination of three coliphages in chickens
spellingShingle The influence of the mode of administration in the dissemination of three coliphages in chickens
Oliveira, A.
Bacteriophage
Escherichia coli respiratory infection
Dissemination
Chicken
Science & Technology
title_short The influence of the mode of administration in the dissemination of three coliphages in chickens
title_full The influence of the mode of administration in the dissemination of three coliphages in chickens
title_fullStr The influence of the mode of administration in the dissemination of three coliphages in chickens
title_full_unstemmed The influence of the mode of administration in the dissemination of three coliphages in chickens
title_sort The influence of the mode of administration in the dissemination of three coliphages in chickens
author Oliveira, A.
author_facet Oliveira, A.
Sereno, R.
Nicolau, Ana
Azeredo, Joana
author_role author
author2 Sereno, R.
Nicolau, Ana
Azeredo, Joana
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, A.
Sereno, R.
Nicolau, Ana
Azeredo, Joana
dc.subject.por.fl_str_mv Bacteriophage
Escherichia coli respiratory infection
Dissemination
Chicken
Science & Technology
topic Bacteriophage
Escherichia coli respiratory infection
Dissemination
Chicken
Science & Technology
description Escherichia coli can cause severe respiratory and systemic infections in chickens, and it is often associated with significant economic losses in the poultry industry. Bacteriophages (phages) have been shown to be potential alternatives to the antibiotics in the treatment of bacterial infections. To accomplish that, phage particles must be able to reach and remain active in the infected organs. The present work aims at evaluating the effect of the route of administration and the dosage in the dissemination of 3 coliphages in the chicken’s organs. In vivo trials were conducted by infecting chickens orally, spray, and i.m. with 106, 107, and 108 plaque-forming units/mL suspensions of 3 lytic phages: phi F78E (Myoviridae), phi F258E (Siphoviridae), and phi F61E (Myoviridae). Birds were killed 3, 10, and 24 h after challenge and the phage titer was measured in lungs and air sacs membranes, liver, duodenum, and spleen. When administered by spray, the 3 phages reached the respiratory tract within 3 h. Oral administration also allowed all phages to be recovered in lungs, but only phi F78E was recovered from the duodenum, the liver, and the spleen. These differences can be explained by the possible replication of phi F78E in commensal E. coli strains present in the chicken gut, thus leading to a higher concentration of this phage in the intestines that resulted in systemic circulation of phage with consequent phage in organs. When phages were administered i.m., they were found in all of the collected organs. Despite this better response, i.m. administration is a nonpracticable way of protecting a large number of birds in a poultry unit. In general, the results suggest that oral administration and spray allowed phages to reach and to remain active in the respiratory tract and can, therefore, be considered promising administration routes to treat respiratory E. coli infections in the poultry industry.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
2009-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9470
url http://hdl.handle.net/1822/9470
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Poultry Science". ISSN 0032-5791. 88:4 (2009) 728-733.
0032-5791
10.3382/ps.2008-00378
19276415
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Poultry Science Association
publisher.none.fl_str_mv Poultry Science Association
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