Isolation and characterization of bacteriophages for avian pathogenic E. coli strains

Detalhes bibliográficos
Autor(a) principal: Oliveira, A.
Data de Publicação: 2009
Outros Autores: Sillankorva, Sanna, Quinta, R., Henriques, Ana Filipa Frutuoso Mendes, Sereno, R., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9518
Resumo: Aims: To isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic Escherichia coli (APEC) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens. Methods and Results: Bacteriophages were isolated from poultry sewage and tested against 148 O-serotyped APEC strains. The morphological characterization of the bacteriophages was made by transmission electronic microscopy (TEM) observations and the genetic comparison between bacteriophages DNA was performed by restriction fragment length polymorphism (RFLP) patterns. Results showed that 70·5% of the tested E. coli strains were sensitive to a combination of three of the five isolated phages, that seemed to be virulent and taxonomically belong to the Caudovirales order. Two of them look like 16–19, T4-like phages (Myoviridae) and the third is a T1-like phage and belongs to Syphoviridae family. All of them are genetically different. Conclusions: It was possible to obtain a combination of three different lytic bacteriophages with broad lytic spectra against the most prevalent O-serotypes of APEC. Significance and Impact of the Study: Data reported in this study, presents an in vitro well studied phage product to be used as antimicrobial agent to treat colibacillosis in poultry industry.
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spelling Isolation and characterization of bacteriophages for avian pathogenic E. coli strainsBacteriophageColibacillosisEscherichia coliPoultryTherapyScience & TechnologyAims: To isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic Escherichia coli (APEC) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens. Methods and Results: Bacteriophages were isolated from poultry sewage and tested against 148 O-serotyped APEC strains. The morphological characterization of the bacteriophages was made by transmission electronic microscopy (TEM) observations and the genetic comparison between bacteriophages DNA was performed by restriction fragment length polymorphism (RFLP) patterns. Results showed that 70·5% of the tested E. coli strains were sensitive to a combination of three of the five isolated phages, that seemed to be virulent and taxonomically belong to the Caudovirales order. Two of them look like 16–19, T4-like phages (Myoviridae) and the third is a T1-like phage and belongs to Syphoviridae family. All of them are genetically different. Conclusions: It was possible to obtain a combination of three different lytic bacteriophages with broad lytic spectra against the most prevalent O-serotypes of APEC. Significance and Impact of the Study: Data reported in this study, presents an in vitro well studied phage product to be used as antimicrobial agent to treat colibacillosis in poultry industry.Fundação para a Ciência e a Tecnologia (FCT)The Society for Applied MicrobiologyUniversidade do MinhoOliveira, A.Sillankorva, SannaQuinta, R.Henriques, Ana Filipa Frutuoso MendesSereno, R.Azeredo, Joana2009-062009-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9518eng"Journal of Applied Microbiology". ISSN 1364-5072. 106:6 (June 2009) 1919-1927.1364-507210.1111/j.1365-2672.2009.04145.x19239552info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:42Zoai:repositorium.sdum.uminho.pt:1822/9518Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:16.285729Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
title Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
spellingShingle Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
Oliveira, A.
Bacteriophage
Colibacillosis
Escherichia coli
Poultry
Therapy
Science & Technology
title_short Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
title_full Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
title_fullStr Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
title_full_unstemmed Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
title_sort Isolation and characterization of bacteriophages for avian pathogenic E. coli strains
author Oliveira, A.
author_facet Oliveira, A.
Sillankorva, Sanna
Quinta, R.
Henriques, Ana Filipa Frutuoso Mendes
Sereno, R.
Azeredo, Joana
author_role author
author2 Sillankorva, Sanna
Quinta, R.
Henriques, Ana Filipa Frutuoso Mendes
Sereno, R.
Azeredo, Joana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, A.
Sillankorva, Sanna
Quinta, R.
Henriques, Ana Filipa Frutuoso Mendes
Sereno, R.
Azeredo, Joana
dc.subject.por.fl_str_mv Bacteriophage
Colibacillosis
Escherichia coli
Poultry
Therapy
Science & Technology
topic Bacteriophage
Colibacillosis
Escherichia coli
Poultry
Therapy
Science & Technology
description Aims: To isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic Escherichia coli (APEC) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens. Methods and Results: Bacteriophages were isolated from poultry sewage and tested against 148 O-serotyped APEC strains. The morphological characterization of the bacteriophages was made by transmission electronic microscopy (TEM) observations and the genetic comparison between bacteriophages DNA was performed by restriction fragment length polymorphism (RFLP) patterns. Results showed that 70·5% of the tested E. coli strains were sensitive to a combination of three of the five isolated phages, that seemed to be virulent and taxonomically belong to the Caudovirales order. Two of them look like 16–19, T4-like phages (Myoviridae) and the third is a T1-like phage and belongs to Syphoviridae family. All of them are genetically different. Conclusions: It was possible to obtain a combination of three different lytic bacteriophages with broad lytic spectra against the most prevalent O-serotypes of APEC. Significance and Impact of the Study: Data reported in this study, presents an in vitro well studied phage product to be used as antimicrobial agent to treat colibacillosis in poultry industry.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
2009-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9518
url http://hdl.handle.net/1822/9518
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Applied Microbiology". ISSN 1364-5072. 106:6 (June 2009) 1919-1927.
1364-5072
10.1111/j.1365-2672.2009.04145.x
19239552
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Society for Applied Microbiology
publisher.none.fl_str_mv The Society for Applied Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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