Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Detalhes bibliográficos
Autor(a) principal: Vieira, Patrícia Manuela
Data de Publicação: 2015
Outros Autores: Mederle, Narcisa, Lobo, Maria Luísa, Imre, Kálmán, Mederle, Ovidiu, Xiao, Lihua, Darabus, Gheorghe, Matos, Olga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.14411/fp.2015.002
Resumo: WOS:000364827200002
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spelling Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in RomaniaCalvesCryptosporidium parvumEastern europeGP60 variabilityManMolecular epidemiologyParasitologyBiochemistry, Genetics and Molecular Biology (miscellaneous)SDG 3 - Good Health and Well-beingWOS:000364827200002To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed.Instituto de Higiene e Medicina Tropical (IHMT)RUNVieira, Patrícia ManuelaMederle, NarcisaLobo, Maria LuísaImre, KálmánMederle, OvidiuXiao, LihuaDarabus, GheorgheMatos, Olga2018-05-14T22:02:45Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttps://doi.org/10.14411/fp.2015.002eng0015-5683PURE: 1733292http://www.scopus.com/inward/record.url?scp=84929401877&partnerID=8YFLogxKhttps://doi.org/10.14411/fp.2015.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:31Zoai:run.unl.pt:10362/36952Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:43.704717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
title Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
spellingShingle Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
Vieira, Patrícia Manuela
Calves
Cryptosporidium parvum
Eastern europe
GP60 variability
Man
Molecular epidemiology
Parasitology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
SDG 3 - Good Health and Well-being
title_short Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
title_full Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
title_fullStr Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
title_full_unstemmed Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
title_sort Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania
author Vieira, Patrícia Manuela
author_facet Vieira, Patrícia Manuela
Mederle, Narcisa
Lobo, Maria Luísa
Imre, Kálmán
Mederle, Ovidiu
Xiao, Lihua
Darabus, Gheorghe
Matos, Olga
author_role author
author2 Mederle, Narcisa
Lobo, Maria Luísa
Imre, Kálmán
Mederle, Ovidiu
Xiao, Lihua
Darabus, Gheorghe
Matos, Olga
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Vieira, Patrícia Manuela
Mederle, Narcisa
Lobo, Maria Luísa
Imre, Kálmán
Mederle, Ovidiu
Xiao, Lihua
Darabus, Gheorghe
Matos, Olga
dc.subject.por.fl_str_mv Calves
Cryptosporidium parvum
Eastern europe
GP60 variability
Man
Molecular epidemiology
Parasitology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
SDG 3 - Good Health and Well-being
topic Calves
Cryptosporidium parvum
Eastern europe
GP60 variability
Man
Molecular epidemiology
Parasitology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
SDG 3 - Good Health and Well-being
description WOS:000364827200002
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2018-05-14T22:02:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.14411/fp.2015.002
url https://doi.org/10.14411/fp.2015.002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0015-5683
PURE: 1733292
http://www.scopus.com/inward/record.url?scp=84929401877&partnerID=8YFLogxK
https://doi.org/10.14411/fp.2015.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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