Interlaboratory exercise for the analysis of carotenoids and related compounds in dried mango fruit (Mangifera indica L.)

• Autor(a) principal: Villacís-Chiriboga, José
• Data de Publicação: 2022
• Outros Autores: Jacobs, Griet, Van Camp, John, Elst, Kathy, Ruales, Jenny, Marcillo-Parra, Verónica, Volker, Böhmg, Bunea, Andrea, Cirlini, Martina, Craft, Neal, De Meulenaer, Bruno, Graça Dias, M., Lazzarino, Giacomo, Meléndez-Martínez, Antonio J., Versloot, Pieter, Mercadante, Adriana Z., Olmedilla-Alonso, Begoña, Ortiz-Ulloa, Johana, Stinco, lCarla M., Voorspoels, Stefan
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.18/8429
• topic: Dried Mango Fruit; Mangifera indica L.; Analysis of Carotenoids; Carotenoids; Analytical Techniques; Interlaboratory Comparison; Analytical Procedures; Extraction and Quantification; Interlaboratory Exercise; Reference Material; Isomers; Interlaboratory Analysis; Lipophilic Compounds; Liquid Chromatography; Quality Assurance; Composição dos Alimentos

An interlaboratory comparison was done for the analysis of carotenoids in freeze-dried mango. The study was performed from July to September 2018. Mango fruit was freeze-dried, homogenized, and packaged under vacuum conditions in portions of 6 g (test sample). Two test samples were sent to the participating laboratories for analysis. Laboratory results were rated using Z-scores in accordance with ISO 13528 and ISO 17043. The standard deviation for proficiency assessment (also called target standard deviation) was determined using a modified Horwitz function and varied between 10% and 25%, depending on the analyte. Out of 14 laboratories from 10 different countries, 9 laboratories (64%) obtained a satisfactory performance (Z ≤ 2) for the analysis of β-carotene. While for 7 laboratories that analyzed α-carotene, (9Z)-β-carotene, β-cryptoxanthin, and zeaxanthin, 4 laboratories (57%) obtained a satisfactory performance. However, only 2 laboratories out of 7 (29%) obtained a satisfactory performance for lutein. Based on the comparability of the analytical results, this study concludes that freeze-dried mango pulp can be used as a reference material for the analysis of α and β-carotene, (9Z)-β-carotene, β-cryptoxanthin, and zeaxanthin by applying different analytical procedures for their extraction and quantification.