Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry

Detalhes bibliográficos
Autor(a) principal: Novak, J.
Data de Publicação: 2007
Outros Autores: Basarova, G., Teixeira, J. A., Vicente, A. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/56407
Resumo: The vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability.
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spelling Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometryCell cyclefluorescent methodsproteinsyeast glycogenyeast propagationScience & TechnologyThe vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability.The Socrates Erasmus program is acknowledged for the financial support of the research carried out in Portugal. We thank UNICER – Bebidas de Portugal S.A. brewery in Portugal for technical support. The authors would like to thank Prof. Gabriela Basarova for her long term support and the helpful discussions.info:eu-repo/semantics/publishedVersionInstitute of Brewing & DistillingUniversidade do MinhoNovak, J.Basarova, G.Teixeira, J. A.Vicente, A. A.20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56407engNovak, J.; Basarova, G.; Teixeira, José A.; Vicente, António A., Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry. Journal of the Institute of Brewing, 113(3), 249-255, 2007.0046-975010.1002/j.2050-0416.2007.tb00284.xhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2050-0416info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:26Zoai:repositorium.sdum.uminho.pt:1822/56407Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:44.122009Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
title Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
spellingShingle Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
Novak, J.
Cell cycle
fluorescent methods
proteins
yeast glycogen
yeast propagation
Science & Technology
title_short Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
title_full Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
title_fullStr Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
title_full_unstemmed Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
title_sort Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry
author Novak, J.
author_facet Novak, J.
Basarova, G.
Teixeira, J. A.
Vicente, A. A.
author_role author
author2 Basarova, G.
Teixeira, J. A.
Vicente, A. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Novak, J.
Basarova, G.
Teixeira, J. A.
Vicente, A. A.
dc.subject.por.fl_str_mv Cell cycle
fluorescent methods
proteins
yeast glycogen
yeast propagation
Science & Technology
topic Cell cycle
fluorescent methods
proteins
yeast glycogen
yeast propagation
Science & Technology
description The vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/56407
url http://hdl.handle.net/1822/56407
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Novak, J.; Basarova, G.; Teixeira, José A.; Vicente, António A., Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry. Journal of the Institute of Brewing, 113(3), 249-255, 2007.
0046-9750
10.1002/j.2050-0416.2007.tb00284.x
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2050-0416
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Institute of Brewing & Distilling
publisher.none.fl_str_mv Institute of Brewing & Distilling
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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