Nanocellulose- based biosensor for colorimetric detection of glucose
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66148 |
Resumo: | This work reports for the first time a colorimetric based biosensor using nanocellulose (NC) based supports drop-deposited onto a cellulose paper substrate for glucose detection in point-of-care. For this purpose, microcrystalline cellulose (MCC) samples were oxidized with 2,2,6,6-tetramethylpiperidine-N-oxyl radical (TEMPO), sodium hypochlorite, and potassium bromide, to produce NC that corresponded to carboxyl- NC. For the characterization, we used several methods: TEM, FTIR and conductometric titration. In all samples, the primary alcohol groups were selectively oxidized into carboxyl groups, provided the sodium hypochlorite is added dropwise and the reaction is performed at constant pH?10. Carboxyl- NC was further casted on a cellulose substrate and used as support for glucose oxidase (GOx), horseradish peroxidase (HRP) and 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) reactions, aiming to yield a coloured detection system for glucose. The sensing system was generated by integrating GOx on the carboxyl- NC /cellulose substrate. Upon reaction with glucose, the enzyme produced hydrogen peroxide, which was converted into a blue-coloured product by reaction with HRP and the chromogenic reagent ABTS. The test-strip was calibrated by incubating it in different concentrations of glucose. The colours obtained were further analysed by a suitable image analysis software. Linear response for glucose ranged 1.5 to 13.0?mM. Overall, this new test-strip used renewable material for glucose determination, which is an advantage when compared to other systems that require more complex technological approaches. Moreover, it was found that Carboxyl- NC improved the colour homogeneity of the test-strip and the intrinsic linear response of concentration range. |
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Nanocellulose- based biosensor for colorimetric detection of glucoseNanocelluloseTEMPO-based oxidationGlucose oxidaseColorimetricTest-stripBiosensorScience & TechnologyThis work reports for the first time a colorimetric based biosensor using nanocellulose (NC) based supports drop-deposited onto a cellulose paper substrate for glucose detection in point-of-care. For this purpose, microcrystalline cellulose (MCC) samples were oxidized with 2,2,6,6-tetramethylpiperidine-N-oxyl radical (TEMPO), sodium hypochlorite, and potassium bromide, to produce NC that corresponded to carboxyl- NC. For the characterization, we used several methods: TEM, FTIR and conductometric titration. In all samples, the primary alcohol groups were selectively oxidized into carboxyl groups, provided the sodium hypochlorite is added dropwise and the reaction is performed at constant pH?10. Carboxyl- NC was further casted on a cellulose substrate and used as support for glucose oxidase (GOx), horseradish peroxidase (HRP) and 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) reactions, aiming to yield a coloured detection system for glucose. The sensing system was generated by integrating GOx on the carboxyl- NC /cellulose substrate. Upon reaction with glucose, the enzyme produced hydrogen peroxide, which was converted into a blue-coloured product by reaction with HRP and the chromogenic reagent ABTS. The test-strip was calibrated by incubating it in different concentrations of glucose. The colours obtained were further analysed by a suitable image analysis software. Linear response for glucose ranged 1.5 to 13.0?mM. Overall, this new test-strip used renewable material for glucose determination, which is an advantage when compared to other systems that require more complex technological approaches. Moreover, it was found that Carboxyl- NC improved the colour homogeneity of the test-strip and the intrinsic linear response of concentration range.The Portuguese Science and Technology Foundation, I.P. (FCT), isacknowledged for the financial support through the project reference P2020 -PTDC/AAG-TEC/5400/2014 (also POCI-01-0145-FEDER-016637, FEDER, COMPETE, POCI) and POCTEP/INTERREG is also acknowledged through the project 2QBioneuro, Impulso de una red deI + i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas. MCCGC acknowledges FCT for the PhD grant SFRH/BD/131959/2017. The Portuguese Foundation for Science andTechnology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorteoperation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund underthe scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoKatrin NeubauerovaCarneiro, MarianaRodrigues, L. R.Moreira, FelisminaSales, M. G. F.2020-082020-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66148engKatrin Neubauerova; Carneiro, Mariana; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. G. F., Nanocellulose- based biosensor for colorimetric detection of glucose. Sensing and Bio-Sensing Research, 29(100368), 20202214-180410.1016/j.sbsr.2020.100368https://www.journals.elsevier.com/sensing-and-bio-sensing-researchinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:39Zoai:repositorium.sdum.uminho.pt:1822/66148Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:15.845247Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Nanocellulose- based biosensor for colorimetric detection of glucose |
title |
Nanocellulose- based biosensor for colorimetric detection of glucose |
spellingShingle |
Nanocellulose- based biosensor for colorimetric detection of glucose Katrin Neubauerova Nanocellulose TEMPO-based oxidation Glucose oxidase Colorimetric Test-strip Biosensor Science & Technology |
title_short |
Nanocellulose- based biosensor for colorimetric detection of glucose |
title_full |
Nanocellulose- based biosensor for colorimetric detection of glucose |
title_fullStr |
Nanocellulose- based biosensor for colorimetric detection of glucose |
title_full_unstemmed |
Nanocellulose- based biosensor for colorimetric detection of glucose |
title_sort |
Nanocellulose- based biosensor for colorimetric detection of glucose |
author |
Katrin Neubauerova |
author_facet |
Katrin Neubauerova Carneiro, Mariana Rodrigues, L. R. Moreira, Felismina Sales, M. G. F. |
author_role |
author |
author2 |
Carneiro, Mariana Rodrigues, L. R. Moreira, Felismina Sales, M. G. F. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Katrin Neubauerova Carneiro, Mariana Rodrigues, L. R. Moreira, Felismina Sales, M. G. F. |
dc.subject.por.fl_str_mv |
Nanocellulose TEMPO-based oxidation Glucose oxidase Colorimetric Test-strip Biosensor Science & Technology |
topic |
Nanocellulose TEMPO-based oxidation Glucose oxidase Colorimetric Test-strip Biosensor Science & Technology |
description |
This work reports for the first time a colorimetric based biosensor using nanocellulose (NC) based supports drop-deposited onto a cellulose paper substrate for glucose detection in point-of-care. For this purpose, microcrystalline cellulose (MCC) samples were oxidized with 2,2,6,6-tetramethylpiperidine-N-oxyl radical (TEMPO), sodium hypochlorite, and potassium bromide, to produce NC that corresponded to carboxyl- NC. For the characterization, we used several methods: TEM, FTIR and conductometric titration. In all samples, the primary alcohol groups were selectively oxidized into carboxyl groups, provided the sodium hypochlorite is added dropwise and the reaction is performed at constant pH?10. Carboxyl- NC was further casted on a cellulose substrate and used as support for glucose oxidase (GOx), horseradish peroxidase (HRP) and 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) reactions, aiming to yield a coloured detection system for glucose. The sensing system was generated by integrating GOx on the carboxyl- NC /cellulose substrate. Upon reaction with glucose, the enzyme produced hydrogen peroxide, which was converted into a blue-coloured product by reaction with HRP and the chromogenic reagent ABTS. The test-strip was calibrated by incubating it in different concentrations of glucose. The colours obtained were further analysed by a suitable image analysis software. Linear response for glucose ranged 1.5 to 13.0?mM. Overall, this new test-strip used renewable material for glucose determination, which is an advantage when compared to other systems that require more complex technological approaches. Moreover, it was found that Carboxyl- NC improved the colour homogeneity of the test-strip and the intrinsic linear response of concentration range. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08 2020-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66148 |
url |
http://hdl.handle.net/1822/66148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Katrin Neubauerova; Carneiro, Mariana; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. G. F., Nanocellulose- based biosensor for colorimetric detection of glucose. Sensing and Bio-Sensing Research, 29(100368), 2020 2214-1804 10.1016/j.sbsr.2020.100368 https://www.journals.elsevier.com/sensing-and-bio-sensing-research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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