Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/110348 |
Resumo: | Diabetes Mellitus is a metabolic disease that affects 422 million people worldwide and is directly responsible for 1.6 M deaths per year, with an increasing incidence especially in low – and middleincome countries. It is characterized by high-blood glucose levels that may lead to severe damages on the patients’ body tissues as blood vessels, eyes, heart, etc. The need to constantly monitor the blood glucose levels means a daily invasive blood collection that can be very stressful for the patient and frequently leads to a non-acceptance of the disease and the treatments. For this reason, glucose presence in other biofluids rather than blood is increasingly being studied, and it is possible to stablish a relation between the sweat glucose concentration values and the blood glucose levels in diabetic patients. Different enzymatic-based and electrochemical approaches have been taken for monitoring the low-glucose concentrations present in sweat, however most of these techniques are expensive and often relies on complex analysis of the results. For these reasons, in this work was developed a non-enzymatic paper-based colorimetric sensor for the detection of glucose in the range of hyperglycemic sweat glucose values (between 0.1 and 1 mM). The sensor is based on the glucose reduction of silver ions around disperse AuNPs adsorved on paper fibers, forming Au@Ag core-shell bimetallic NPs. The Ag-shell thickness increases with glucose concentration, resulting on colorimetric differences that may be quantified through digital analysis. Paper was utilized as substrate using the Lab-on-paper technology, where the deposition sites for glucose testing were defined by wax barriers diffused along the paper. Paper was chosen for being an abundant, biocompatible and low-cost material with good mechanical properties. Through RGB intensity measurement of the colorimetric results, a linear relation for glucose levels in a range from 0.12 to 1 mM was obtained. |
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Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paperPaper biosensorcolorimetric assaybimetallic nanoparticlessweat glucoseenzyme-freeDomínio/Área Científica::Engenharia e Tecnologia::NanotecnologiaDiabetes Mellitus is a metabolic disease that affects 422 million people worldwide and is directly responsible for 1.6 M deaths per year, with an increasing incidence especially in low – and middleincome countries. It is characterized by high-blood glucose levels that may lead to severe damages on the patients’ body tissues as blood vessels, eyes, heart, etc. The need to constantly monitor the blood glucose levels means a daily invasive blood collection that can be very stressful for the patient and frequently leads to a non-acceptance of the disease and the treatments. For this reason, glucose presence in other biofluids rather than blood is increasingly being studied, and it is possible to stablish a relation between the sweat glucose concentration values and the blood glucose levels in diabetic patients. Different enzymatic-based and electrochemical approaches have been taken for monitoring the low-glucose concentrations present in sweat, however most of these techniques are expensive and often relies on complex analysis of the results. For these reasons, in this work was developed a non-enzymatic paper-based colorimetric sensor for the detection of glucose in the range of hyperglycemic sweat glucose values (between 0.1 and 1 mM). The sensor is based on the glucose reduction of silver ions around disperse AuNPs adsorved on paper fibers, forming Au@Ag core-shell bimetallic NPs. The Ag-shell thickness increases with glucose concentration, resulting on colorimetric differences that may be quantified through digital analysis. Paper was utilized as substrate using the Lab-on-paper technology, where the deposition sites for glucose testing were defined by wax barriers diffused along the paper. Paper was chosen for being an abundant, biocompatible and low-cost material with good mechanical properties. Through RGB intensity measurement of the colorimetric results, a linear relation for glucose levels in a range from 0.12 to 1 mM was obtained.Fortunato, ElviraRUNSerrano, Laura Ramos2021-01-18T12:14:23Z2020-0720202020-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/110348enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:53:22Zoai:run.unl.pt:10362/110348Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:41:19.468694Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
title |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
spellingShingle |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper Serrano, Laura Ramos Paper biosensor colorimetric assay bimetallic nanoparticles sweat glucose enzyme-free Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
title_short |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
title_full |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
title_fullStr |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
title_full_unstemmed |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
title_sort |
Bimetallic nanoparticles for highly sensitive colorimetric detection of glucose on paper |
author |
Serrano, Laura Ramos |
author_facet |
Serrano, Laura Ramos |
author_role |
author |
dc.contributor.none.fl_str_mv |
Fortunato, Elvira RUN |
dc.contributor.author.fl_str_mv |
Serrano, Laura Ramos |
dc.subject.por.fl_str_mv |
Paper biosensor colorimetric assay bimetallic nanoparticles sweat glucose enzyme-free Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
topic |
Paper biosensor colorimetric assay bimetallic nanoparticles sweat glucose enzyme-free Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
description |
Diabetes Mellitus is a metabolic disease that affects 422 million people worldwide and is directly responsible for 1.6 M deaths per year, with an increasing incidence especially in low – and middleincome countries. It is characterized by high-blood glucose levels that may lead to severe damages on the patients’ body tissues as blood vessels, eyes, heart, etc. The need to constantly monitor the blood glucose levels means a daily invasive blood collection that can be very stressful for the patient and frequently leads to a non-acceptance of the disease and the treatments. For this reason, glucose presence in other biofluids rather than blood is increasingly being studied, and it is possible to stablish a relation between the sweat glucose concentration values and the blood glucose levels in diabetic patients. Different enzymatic-based and electrochemical approaches have been taken for monitoring the low-glucose concentrations present in sweat, however most of these techniques are expensive and often relies on complex analysis of the results. For these reasons, in this work was developed a non-enzymatic paper-based colorimetric sensor for the detection of glucose in the range of hyperglycemic sweat glucose values (between 0.1 and 1 mM). The sensor is based on the glucose reduction of silver ions around disperse AuNPs adsorved on paper fibers, forming Au@Ag core-shell bimetallic NPs. The Ag-shell thickness increases with glucose concentration, resulting on colorimetric differences that may be quantified through digital analysis. Paper was utilized as substrate using the Lab-on-paper technology, where the deposition sites for glucose testing were defined by wax barriers diffused along the paper. Paper was chosen for being an abundant, biocompatible and low-cost material with good mechanical properties. Through RGB intensity measurement of the colorimetric results, a linear relation for glucose levels in a range from 0.12 to 1 mM was obtained. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07 2020 2020-07-01T00:00:00Z 2021-01-18T12:14:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/110348 |
url |
http://hdl.handle.net/10362/110348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799138026812604416 |