Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/20734 |
Resumo: | Techniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access,lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels.Visualizations and measurements of the deformation of RBCs flowing through hyperbolic,smooth,and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability. |
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Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurementsRed blood cellsDeformation indexMicrofluidic devicesAir bubblesDropletsBloo dflowTechniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access,lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels.Visualizations and measurements of the deformation of RBCs flowing through hyperbolic,smooth,and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability.MDPIBiblioteca Digital do IPBBento, DavidRodrigues, Raquel OliveiraFaustino, VeraPinho, DianaFernandes, Carla S.Pereira, Ana I.Garcia, ValdemarMiranda, João MárioLima, Rui A.2020-02-27T15:24:16Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/20734engBento, David; Rodrigues, Raquel O.; Faustino, Vera; Pinho, Diana; Fernandes, Carla S.; Pereira, Ana I.; Garcia, Valdemar; Miranda, João M.; Lima, R. (2018). Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements. Micromachines. ISSN 2072-666X. 9:1512072-666X10.3390/mi9040151info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:46:54Zoai:bibliotecadigital.ipb.pt:10198/20734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:11:34.051578Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
title |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
spellingShingle |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements Bento, David Red blood cells Deformation index Microfluidic devices Air bubbles Droplets Bloo dflow |
title_short |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
title_full |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
title_fullStr |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
title_full_unstemmed |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
title_sort |
Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements |
author |
Bento, David |
author_facet |
Bento, David Rodrigues, Raquel Oliveira Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João Mário Lima, Rui A. |
author_role |
author |
author2 |
Rodrigues, Raquel Oliveira Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João Mário Lima, Rui A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Bento, David Rodrigues, Raquel Oliveira Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João Mário Lima, Rui A. |
dc.subject.por.fl_str_mv |
Red blood cells Deformation index Microfluidic devices Air bubbles Droplets Bloo dflow |
topic |
Red blood cells Deformation index Microfluidic devices Air bubbles Droplets Bloo dflow |
description |
Techniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access,lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels.Visualizations and measurements of the deformation of RBCs flowing through hyperbolic,smooth,and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2020-02-27T15:24:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/20734 |
url |
http://hdl.handle.net/10198/20734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bento, David; Rodrigues, Raquel O.; Faustino, Vera; Pinho, Diana; Fernandes, Carla S.; Pereira, Ana I.; Garcia, Valdemar; Miranda, João M.; Lima, R. (2018). Deformation of red blood cells, air bubbles and droplets in microfluidic devices: flow visualizations and measurements. Micromachines. ISSN 2072-666X. 9:151 2072-666X 10.3390/mi9040151 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135384612896768 |