Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/23069 |
Resumo: | This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HApsuggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μg ml−1), although its cellular uptake behavior. Therefore, the prepared HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties. |
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Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluationNanohydroxyapatiteCalcium PhosphatesOscillatory Flow ReactorMineralizationSBFThis work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HApsuggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μg ml−1), although its cellular uptake behavior. Therefore, the prepared HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.IOP PublishingVeritati - Repositório Institucional da Universidade Católica PortuguesaCastro, FilipaRibeiro, Viviana. P.Ferreira, AntónioOliveira, Ana L.Reis, Rui L.Teixeira, José A.Rocha, Fernando2017-10-13T08:14:46Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/23069engCASTRO, Filipa; RIBEIRO, Viviana. P.; FERREIRA, António; OLIVEIRA, Ana L.; REIS, Rui L.; TEIXEIRA, José A.; ROCHA, Fernando - Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation. Materials Research Express. ISSN 2053-1591. Vol. 3, n.º 7 (2016), 13 p. (075404)10.1088/2053-1591/3/7/07540484981517872000380852000039info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-05T01:34:59Zoai:repositorio.ucp.pt:10400.14/23069Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:17:21.869133Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
title |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
spellingShingle |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation Castro, Filipa Nanohydroxyapatite Calcium Phosphates Oscillatory Flow Reactor Mineralization SBF |
title_short |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
title_full |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
title_fullStr |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
title_full_unstemmed |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
title_sort |
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation |
author |
Castro, Filipa |
author_facet |
Castro, Filipa Ribeiro, Viviana. P. Ferreira, António Oliveira, Ana L. Reis, Rui L. Teixeira, José A. Rocha, Fernando |
author_role |
author |
author2 |
Ribeiro, Viviana. P. Ferreira, António Oliveira, Ana L. Reis, Rui L. Teixeira, José A. Rocha, Fernando |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Castro, Filipa Ribeiro, Viviana. P. Ferreira, António Oliveira, Ana L. Reis, Rui L. Teixeira, José A. Rocha, Fernando |
dc.subject.por.fl_str_mv |
Nanohydroxyapatite Calcium Phosphates Oscillatory Flow Reactor Mineralization SBF |
topic |
Nanohydroxyapatite Calcium Phosphates Oscillatory Flow Reactor Mineralization SBF |
description |
This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HApsuggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μg ml−1), although its cellular uptake behavior. Therefore, the prepared HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2017-10-13T08:14:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/23069 |
url |
http://hdl.handle.net/10400.14/23069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
CASTRO, Filipa; RIBEIRO, Viviana. P.; FERREIRA, António; OLIVEIRA, Ana L.; REIS, Rui L.; TEIXEIRA, José A.; ROCHA, Fernando - Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation. Materials Research Express. ISSN 2053-1591. Vol. 3, n.º 7 (2016), 13 p. (075404) 10.1088/2053-1591/3/7/075404 84981517872 000380852000039 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131860364689408 |